In situ Fluoride Retention in Enamel and Dentine after the Use of an Amine Fluoride Dentifrice and Amine Fluoride/Sodium Fluoride Mouthrinse

Abstract

The aim of this in situ investigation was to study the effect of an amine fluoride/sodium fluoride mouthrinse (total F = 250 ppm) in addition to an amine fluoride dentifrice (F = 1,250 ppm) on the amount of acquired fluoride in enamel and dentine. In the partial prosthesis of 12 participants a combined specimen of slightly demineralized enamel and dentine was placed. During two consecutive experiments, each lasting 3 weeks, the participants used an amine fluoride dentifrice alone or in combination with a fluoride mouthrinse. After the in situ period the specimens were retrieved and both the enamel and the dentine specimens were analysed for the amount of KOH–soluble fluoride and structurally bound fluoride. The results showed a significant increase in both KOH–soluble and structurally bound fluoride in enamel and dentine when a fluoride mouthrinse was used. Whether the rinsing procedure was performed immediately after toothbrushing or with a delay of 2 h did not influence the results. Furthermore it was shown that dentine acquired substantially more fluoride than enamel during the experimental period. The results indicate that a fluoride mouthrinse used in addition to a fluoride dentifrice may have a beneficial effect on the protection of enamel and dentine against caries.

Formulation, Development and Characterization of Sustained Release Varnishes Containing Amine and Stannous Fluorides

“…However, this difference was statistically significant only 90 min after toothbrushing when preceded by mouthrinsing after brushing [Attin and Hellwing, 1996]. High retention of fluoride after a fluoride mouthrinse and AmF dentifrice was found on enamel and dentine [van Strijp, 1999], and especially in lesions [ten Cate et al, 1988]. The present study indicates that AmF and AmF/SnF 2 can be incorporated into SRV while maintaining their antibacterial effect.…”

“…Similar to our results Shani et al [2000] have also found that AmF has maintained high antibacterial activity although large amounts of bacteria were initially adsorbed onto HA saturated with an AmF solution. The high affinity of AmF to surfaces [Sefton et al, 1996;van Strijp, 1999], coupled with its relatively high affinity to bacteria, facilitates a strong killing effect of bacteria in a biofilm coated with AmF varnish.…”

“…Stannous fluoride (SnF 2 ) is another antibacterial agent which has been proven to be effective against Streptococcus mutans [Mayhew and Brown, 1981]. Both amine fluoride and SnF 2 have a protection effect on enamel and dentine against caries [van Strijp et al, 1999], probably due to the high affinity of the fluoride component to enamel [Chan et al, 1991]. Several dental pharmaceutical applications have used a combination of SnF 2 and AmF.…”

Effect of different frequencies of fluoride dentifrice and mouthrinse administration: an in situ study

“…Several studies testing fluoride therapy on demineralized enamel blocks have shown that fluoride content is dependent on the frequency of topical application. 12,23 One possible explanation for the difference observed between studies using previously demineralized enamel blocks versus sound enamel blocks submitted to cariogenic challenge may be that fluoride uptake in the latter experiments reached a plateau. According to Raven et al 24 the increased porosity and surface area of demineralized enamel increases its reactivity by allowing the fluoride to diffuse more readily into the enamel.…”

“…Mouthrinses are usually recommended for active-caries patients in addition to normal use of fluoride dentifrice. 4 In vivo [6][7][8][9] and in situ studies [10][11][12] have shown that the frequency of topical fluoride applications is of importance to the effectiveness of caries preventive measures. Mouthrinses do not increase the fluoride exposure frequency, since they are usually applied immediately after the toothbrushing procedure.…”