In situ formation of gold nanoparticles in polymer inclusion membrane: Application as platform in a label-free potentiometric immunosensor for Salmonella typhimurium detection

Silva, Nádia F.D.; Magalhães, Júlia M.C.S.; Barroso, M. Fátima; Oliva-Teles, Maria Teresa; Freire, Cristina; Delerue–Matos, Cristina

doi:10.1016/j.talanta.2018.10.024

Cited by 61 publications

(39 citation statements)

References 54 publications

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“…They can generate signal immediately after binding to the recognition element and do not require additional interactions with the labels that provide signal. In this context, many physicochemical types of transducers have been proposed that transform the results of bioreceptor-based binding of selected targets (for example, increase in weight, specific resistance, or surface refractive indices), which are recognized in various ways [29,71,78,89,90].…”

Section: Label-free Biosensorsmentioning

confidence: 99%

“…Optical, electrochemical, electric (piezoelectric), microwave, or (micro) mechanical transducers are promising strategies for recognizing ligand-receptor interaction signals in label-free biosensors used to indicate biological agents or biomonitoring the environment [32,40,80,84]. Among the most powerful detection and analysis tools, widely used in biomedical research and in practical medicine, are biosensors with optical transducers, considered the basic tool for signal perception [3,7,75,89,90].…”

Section: Label-free Biosensorsmentioning

confidence: 99%

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Label-Free Biosensors for Laboratory-Based Diagnostics of Infections: Current Achievements and New Trends

et al. 2020

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Infections pose a serious global public health problem and are a major cause of premature mortality worldwide. One of the most challenging objectives faced by modern medicine is timely and accurate laboratory-based diagnostics of infectious diseases. Being a key factor of timely initiation and success of treatment, it may potentially provide reduction in incidence of a disease, as well as prevent outbreak and spread of dangerous epidemics. The traditional methods of laboratory-based diagnostics of infectious diseases are quite time- and labor-consuming, require expensive equipment and qualified personnel, which restricts their use in case of limited resources. Over the past six decades, diagnostic technologies based on lateral flow immunoassay (LFIA) have been and remain true alternatives to modern laboratory analyzers and have been successfully used to quickly detect molecular ligands in biosubstrates to diagnose many infectious diseases and septic conditions. These devices are considered as simplified formats of modern biosensors. Recent advances in the development of label-free biosensor technologies have made them promising diagnostic tools that combine rapid pathogen indication, simplicity, user-friendliness, operational efficiency, accuracy, and cost effectiveness, with a trend towards creation of portable platforms. These qualities exceed the generally accepted standards of microbiological and immunological diagnostics and open up a broad range of applications of these analytical systems in clinical practice immediately at the site of medical care (point-of-care concept, POC). A great variety of modern nanoarchitectonics of biosensors are based on the use of a broad range of analytical and constructive strategies and identification of various regulatory and functional molecular markers associated with infectious bacterial pathogens. Resolution of the existing biosensing issues will provide rapid development of diagnostic biotechnologies.

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Section: Label-free Biosensorsmentioning

confidence: 99%

Section: Label-free Biosensorsmentioning

confidence: 99%

Label-Free Biosensors for Laboratory-Based Diagnostics of Infections: Current Achievements and New Trends

et al. 2020

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“…Liang P, et al utilized dithiothreitol to fine-tune the surface coverage of oligonucleotide-modified AuNPs for perform cocaine detection in 50% urine [27]. AuNPs formed in-situ on a polymer inclusion membrane (AuNPs-PIM) used as a biocompatible sensing platform for bioreceptor conjugation and the signal-output amplification was implemented on the AuNPs-PIM in a label free biosensor [28]. However, AuNPs were mostly used for in-situ labeling of biomedical sensors and rarely applied in ISEs.…”

Section: -mentioning

confidence: 99%

An all-solid-state NO3- ion-selective electrode with gold nanoparticles solid contact layer and molecularly imprinted polymer membrane

2020

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To improve the single-layer all-solid-state ion selective electrode' defects including poor conductivity of PVC sensitive membrane and interference of water layer between substrate electrode and sensitive membrane, a double-layer all-solid-state ion selective electrode with nanomaterial as the solid contact layer and conductive polymer as the ion sensitive membrane was developed. A gold nanoparticles solid contact layer and a nitrate-doped polypyrrole molecularly imprinted polymer membrane were prepared by electrodeposition. The optimal parameters obtained by electrochemical performance test were 2.5 mmol/L HAuCl 4 electrolyte for solid contact layer and 1800s electrodeposition time for sensitive membrane. The new electrode exhibited a Nernstian response of-50.4 mV/decade and a low detection limit of 5.25×10-5 mol/L. Potentiometric water layer test showed no water film formed between the gold nanoparticles solid contact layer and nitrate-doped polypyrrole molecularly imprinted polymer membrane. The contact angle between droplet and the surface of solid contact layer was 112.35˚and showed good hydrophobic property. Furthermore, the developed electrode exhibited fast response, excellent potential stability and long lifetime. This electrode is suitable for the detection of nitrate concentration in water and liquid fertilizer.

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“…In this work, a simple and cost-effective paper-based potentiometric immunosensing platform was developed and a label-free potentiometric immunosensor for Salmonella thyphimurium (ST) detection, based on surface blocking principle (Gyurcsanyi et al, 2003;Pawlak et al, 2015;Silva et al, 2019;Xu et al, 2005) is presented as a proof-of-concept. This detection principle comprises the regulation of a marker ion flux from or into an ion selective membrane mediated by a specific biorecognition event that takes place near or on its surface, which is able to induce a potentiometric response, which can be used as analytical signal.…”

Section: Introductionmentioning

confidence: 99%

Development of a disposable paper-based potentiometric immunosensor for real-time detection of a foodborne pathogen

Silva

Almeida

Magalhães

et al. 2019

Biosensors and Bioelectronics

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This work reports a new paper-based sensing platform and its application in a label-free potentiometric immunosensor for Salmonella typhimurium detection based on the blocking surface principle. A paper-based strip electrode was integrated with a filter paper pad which acted as a reservoir of the internal solution. The design offers a convenient platform for antibody immobilization and sampling, proving also that is a simple and affordable methodology to control an ionic flux through a polymer membrane. Two different immunosensing interfaces were assembled on the developed paper-strip electrode. The simplest interface relied on direct conjugation of the antibody to the polymer membrane and the second one resorted to an intermediate layer of a polyamidoamine dendrimer, with an ethylenediamine core from the fourth generation. Electrochemical impedance spectroscopy was used to assess the successive interface modification steps and the resulting analytical performance of both immunosensors was compared. For such, the potential shift derived from the blocking effect of the ionic flux caused by antigen-antibody conjugation was correlated with the logarithm of the Salmonella typhimurium concentration in the sample. In optimized conditions, a limit of detection of 5 cells mL −1 was achieved. As a proof-of-concept, the proposed method was applied to apple juice samples, demonstrating to be a suitable prototype to be used in real scenarios in useful time (< 1 h assay).

show abstract

In situ formation of gold nanoparticles in polymer inclusion membrane: Application as platform in a label-free potentiometric immunosensor for Salmonella typhimurium detection

Cited by 61 publications

References 54 publications

Label-Free Biosensors for Laboratory-Based Diagnostics of Infections: Current Achievements and New Trends

Label-Free Biosensors for Laboratory-Based Diagnostics of Infections: Current Achievements and New Trends

An all-solid-state NO3- ion-selective electrode with gold nanoparticles solid contact layer and molecularly imprinted polymer membrane

Development of a disposable paper-based potentiometric immunosensor for real-time detection of a foodborne pathogen

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