In-situ formation of MnO2 nanoparticles on Ru@SiO2 nanospheres as a fluorescent probe for sensitive and rapid detection of glutathione

Selective Determination of Glutathione Using a Highly Emissive Fluorescent Probe Based on a Pyrrolidine-Fused Chlorin

“…Moreover, applications where sub-micromolar GSH concentrations need to be determined can be covered by this chlorin-based fluorescent probe, with a LOD for TCPC:Hg 2+ 1:2 as low as 40 nM. This value, susceptible to further reduction by reducing the TCPC:Hg 2+ ratio, is significantly lower than those found in most of the existing literature using fluorescent probes [ 32 , 74 , 75 , 76 , 77 , 78 , 79 , 80 , 81 ] and very similar to those reporting the lowest values [ 53 , 67 , 75 ]. These results are summarized in Table S1 .…”

“…On the other hand, colorimetric and fluorescent probes have become an excellent alternative for GSH detection due to their simple mechanisms, easy sample preparation, and colour changes with the naked eye when the analyte is present [ 32 , 47 , 48 , 49 , 50 , 51 , 52 , 53 ]. However, taking advantage of these properties requires selective, sensitive and robust methods.…”

ALP-assisted chemical redox cycling signal amplification for ultrasensitive fluorescence detection of DNA methylation

“…1F, the Ru@SiO 2 nanoparticles show a negative charge because silica is negatively charged, which is attributed to the low isoelectric point of silica under neutral or alkaline conditions. 29 After the Ru@SiO 2 surface is modified with MES, the surface charge of Ru@SiO 2 positively shifts. This is because MES contains a sulfonic acid group with a positive charge, which can neutralize the negative charge of the Ru@SiO 2 surface and even change its surface charge.…”

“…Preparation of Ru@SiO 2 @MnO 2 nanocomposites Ru@SiO 2 nanoparticles were first synthesized by the water-inoil microemulsion method in light of our previously reported works. 29 The synthesis was carried out as follows: firstly, 240 µL of 0.1 M Ru(bpy) 3 2+ aqueous solution was mixed with 22.5 mL of cyclohexane, 5.31 mL of Triton-100 and 5.4 mL of 1-hexanol to form a microemulsion under stirring for 30 min. Then, 180 µL of NH 3 •H 2 O was added to the above microemulsion to initiate the polymerization after the addition of 300 µL of TEOS.…”

Electrochemiluminescence Biosensor for Human Papillomavirus DNA Based on Nanoparticle Charge Density Regulation through a Target Activated CRISPR/Cas12a System

“…The modification of the ECL probes by DNA is tedious, and each DNA can be modified with only one ECL probe, the ECL intensity is not high enough. Tris­(2,2′-bipyridyl) ruthenium­(II) chloride hexahydrate-doped SiO 2 nanoparticles (Ru@SiO 2 NPs) have been widely used in the construction of ECL biosensors, − each nanoparticle contains large amount of ECL probe, which causes the enhancement of the ECL intensity, and the silica-based nanostructures can resist dye molecules coming off and improve their stabilities . Furthermore, the surface of the nanoparticles has a large number of silicon hydroxyl groups, which are easily modified with many function groups.…”