A series of acrylamide (AAm) based hydrogels containing acrylic acid (AAc), 2-acrylamido-2-methyl-1-propanesulfonic acid (AMPS) and vinyl imidazole (VI) comonomers were prepared by free radical polymerization. Silver nanoparticles were loaded to hydrogels systems via in situ reduction of silver nitrate in the presence of sodium borohydride as a reducing agent. The synthesized hydrogels and their composites were characterized using FT-IR, SEM, EDX and EDX-Mapping. The antimicrobial activity of hydrogel-silver composite was determined by using well agar and broth dilution tests. In the first stage, four different hydrogel-silver composites were evaluated against six different microorganisms using the well agar technique. The most effective hydrogel-silver composite among all tested was poly (AAm-co-VI-co-AMPS)-Ag, while the most sensitive and resistant microorganisms among all tested were S. cerevisiae and S. aureus respectively. Poly (AAm-co-VI-co-AMPS)-Ag composite was used in modeling the inhibition kinetic of E.coli. The present study displays that hydrogel-silver composite has considerable antimicrobial activity, which deserves further investigation for use in clinic application and industrial processing.2 Graphical Abstract KEYWORDS: Acrylamide based hydrogels, hydrogel-silver composites, antimicrobial activity, inhibition kinetic.