In situ friction measurement on murine cartilage by atomic force microscopy

Coles, Jeffrey M.; Blum, Jason J.; Jay, Gregory D.; Darling, Eric M.; Guilak, Farshid; Zauscher, Stefan

doi:10.1016/j.jbiomech.2007.10.013

Cited by 64 publications

(73 citation statements)

References 56 publications

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“…Measured ECM moduli were greater than those previously reported for the superficial zone (154 kPa versus 44 kPa) of porcine patellar cartilage [66] and the middle/deep zone (90 -106 kPa versus 81 kPa) of porcine articular cartilage [38]. Furthermore, the observed ECM microscale elastic properties decreased with depth from the articular surface.…”

Section: Discussioncontrasting

confidence: 62%

Immunofluorescence-guided atomic force microscopy to measure the micromechanical properties of the pericellular matrix of porcine articular cartilage

Wilusz

DeFrate

Guilak

2012

J. R. Soc. Interface.

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The pericellular matrix (PCM) is a narrow region that is rich in type VI collagen that surrounds each chondrocyte within the extracellular matrix (ECM) of articular cartilage. Previous studies have demonstrated that the chondrocyte micromechanical environment depends on the relative properties of the chondrocyte, its PCM and the ECM. The objective of this study was to measure the influence of type VI collagen on site-specific micromechanical properties of cartilage in situ by combining atomic force microscopy stiffness mapping with immunofluorescence imaging of PCM and ECM regions in cryo-sectioned tissue samples. This method was used to test the hypotheses that PCM biomechanical properties correlate with the presence of type VI collagen and are uniform with depth from the articular surface. Control experiments verified that immunolabelling did not affect the properties of the ECM or PCM. PCM biomechanical properties correlated with the presence of type VI collagen, and matrix regions lacking type VI collagen immediately adjacent to the PCM exhibited higher elastic moduli than regions positive for type VI collagen. PCM elastic moduli were similar in all three zones. Our findings provide further support for type VI collagen in defining the chondrocyte PCM and contributing to its biological and biomechanical properties.

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Section: Discussioncontrasting

confidence: 62%

Immunofluorescence-guided atomic force microscopy to measure the micromechanical properties of the pericellular matrix of porcine articular cartilage

Wilusz

DeFrate

Guilak

2012

J. R. Soc. Interface.

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“…7,44,45 We hypothesize that this relative softness lends itself toward a plowing mechanism that contributed to the observed friction values. 52 TGFb enhances SZP secretion in SZ articular chondrocytes 14,26,27 and may provide an avenue to concomitantly improve mechanical and frictional properties.…”

Section: Figmentioning

confidence: 99%

Surface Zone Articular Chondrocytes Modulate the Bulk and Surface Mechanical Properties of the Tissue-Engineered Cartilage

Peng

McNary

Athanasiou³

et al. 2014

Tissue Engineering Part A

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The central hypothesis of functional tissue engineering is that an engineered construct can serve as a viable replacement tissue in vivo by replicating the structure and function of native tissue. In the case of articular cartilage, this requires the reproduction of the bulk mechanical and surface lubrication properties of native hyaline cartilage. Cartilage tissue engineering has primarily focused on achieving the bulk mechanical properties of native cartilage such as the compressive aggregate modulus and tensile strength. A scaffold-free selfassembling process has been developed that produces engineered cartilage with compressive properties approaching native tissue levels. Thus, the next step in this process is to begin addressing the friction coefficient and wear properties of these engineered constructs. The superficial zone protein (SZP), also known as lubricin or PRG4, is a boundary mode lubricant that is synthesized by surface zone (SZ) articular chondrocytes. Under conditions of high loading and low sliding speeds, SZP reduces friction and wear at the articular surface. The objective of this investigation was to determine whether increasing the proportion of SZ chondrocytes in cartilage constructs, in the absence of external stimuli such as growth factors and mechanical loading, would enhance the secretion of SZP and improve their frictional properties. In this study, cartilage constructs were engineered through a self-assembling process with varying ratios of SZ and middle zone (MZ) chondrocytes (SZ:MZ): 0:100, 25:75, 50:50, 75:25, and 100:0. Constructs containing different ratios of SZ and MZ chondrocytes did not significantly differ in the glycosaminoglycan composition or compressive aggregate modulus. In contrast, tensile properties and collagen content were enhanced in nearly all constructs containing greater amounts of SZ chondrocytes. Increasing the proportion of SZ chondrocytes had the hypothesized effect of improving the synthesis and secretion of SZP. However, increasing the SZ chondrocyte fraction did not significantly reduce the friction coefficient. These results demonstrate that additional factors, such as SZPbinding macromolecules, surface roughness, and adhesion, need to be examined to modulate the lubrication properties of engineered cartilage.

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“…Atomic Force Microscopy (AFM) has been widely used to identify frictional properties of various materials and the role of boundary lubricants at the microscopic level by measuring the surface roughness and the frictional coefficient [21][22][23][24]. Kim et al [25] reported that the friction of a soft contact lens surface was significantly reduced under the lubrication of saline solution, whereas Liu and Bhushan found that using perfluoropolyether (PFPE) as a lubricant could significantly improve the frictional behavior of a silicon surface [26].…”

Section: Introductionmentioning

confidence: 99%

Effect of protein concentrations of bovine serum albumin and γ-globulin on the frictional response of a cobalt-chromium femoral head

Duong

Lee

Cho

et al. 2012

J Mater Sci: Mater Med

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The study aims to identify the concentration-dependent role of bovine serum albumin (BSA) and γ-globulin in the lubricating ability of a cobalt-chromium femoral head. The frictional coefficients of the cobalt-chromium femoral head decreased with increasing BSA concentrations from 10 to 40 mg/ml and showed statistical differences between any of the BSA concentration groups, except between the 30 and 40 mg/ml concentration groups. In γ-globulin, the frictional coefficients significantly decreased at concentrations of 2.5 and 5.0 mg/ml as compared with the PBS control group, but significant increases were observed at 7.5 and 12.5 mg/ml. These results suggest that the friction of the cobalt-chromium femoral head is dependent on the concentration of both BSA and γ-globulin. However, there is a maximum concentration for BSA to act as an effective boundary lubricant, while the lubricating ability of γ-globulin is most effective in the physiological concentration range within human synovial fluid.

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In situ friction measurement on murine cartilage by atomic force microscopy

Cited by 64 publications

References 56 publications

Immunofluorescence-guided atomic force microscopy to measure the micromechanical properties of the pericellular matrix of porcine articular cartilage

Immunofluorescence-guided atomic force microscopy to measure the micromechanical properties of the pericellular matrix of porcine articular cartilage

Surface Zone Articular Chondrocytes Modulate the Bulk and Surface Mechanical Properties of the Tissue-Engineered Cartilage

Effect of protein concentrations of bovine serum albumin and γ-globulin on the frictional response of a cobalt-chromium femoral head

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