In situ gelling hydrogel loaded with berberine liposome for the treatment of biofilm-infected wounds

Abstract

Background: In recent years, the impact of bacterial biofilms on traumatic wounds and the means to combat them have become a major research topic in the field of medicine. The eradication of biofilms formed by bacterial infections in wounds has always been a huge challenge. Herein, we developed a hydrogel with the active ingredient berberine hydrochloride liposomes to disrupt the biofilm and thereby accelerate the healing of infected wounds in mice.Methods: We determined the ability of berberine hydrochloride liposomes to eradicate the biofilm by means of studies such as crystalline violet staining, measuring the inhibition circle, and dilution coating plate method. Encouraged by the in vitro efficacy, we chose to coat the berberine hydrochloride liposomes on the Poloxamer range of in-situ thermosensitive hydrogels to allow fuller contact with the wound surface and sustained efficacy. Eventually, relevant pathological and immunological analyses were carried out on wound tissue from mice treated for 14 days.Results: The final results show that the number of wound tissue biofilms decreases abruptly after treatment and that the various inflammatory factors in them are significantly reduced within a short period. In the meantime, the number of collagen fibers in the treated wound tissue, as well as the proteins involved in healing in the wound tissue, showed significant differences compared to the model group.Conclusion: From the results, we found that berberine liposome gel can accelerate wound healing in Staphylococcus aureus infections by inhibiting the inflammatory response and promoting re-epithelialization as well as vascular regeneration. Our work exemplifies the efficacy of liposomal isolation of toxins. This innovative antimicrobial strategy opens up new perspectives for tackling drug resistance and fighting wound infections.

Silk Sericin and Its Composite Materials with Antibacterial Properties to Enhance Wound Healing: A Review

“…Berberine is an isoquinoline derivative with anti-inflammatory and antimicrobial properties, which has been used for treating wound healing when embedded in vesicle carriers, gel delivery systems, and cream formulations [ 122 , 123 , 124 ]. This method of encapsulating drugs into vesicles and encapsulating vesicles into semi-solid formulations (hydrogels) has been confirmed to be an important strategy to circumvent the drawbacks of nanovesicles, and the sequestration of such systems in polymer matrices provides a means of further controlling the spatial and temporal release of drugs [ 125 , 126 ].…”

pH‐Responsive Bimetallic MOFs ZIF‐8@ZIF‐67 for Enhanced Antibacterial Activity

“…[19] This necessitates the development of novel drug delivery materials to optimize drug efficacy and mitigate antimicrobial resistance. [20] Berberine hydrochloride (BBH) is an isoquinoline alkaloid with diverse biological activities, including antibacterial, anticancer, anti-inflammatory, and antioxidant effects. [21] BBH exhibits broad-spectrum antibacterial activity against various Gram-negative and Gram-positive bacteria, [22] particularly demonstrating pronounced inhibitory effects on Staphylococcus aureus, Streptococcus hemolyticus, Vibrio cholera, Salmonella typhi and Corynebacterium diphtheriae.…”

“…It has been reported that death caused by bacterial infection is the second leading cause of death in the world, and the emergence of antimicrobial resistance (AMR) poses a growing global crisis due to increasing bacterial resistance to existing drugs [19] . This necessitates the development of novel drug delivery materials to optimize drug efficacy and mitigate antimicrobial resistance [20] . Berberine hydrochloride (BBH) is an isoquinoline alkaloid with diverse biological activities, including antibacterial, anticancer, anti‐inflammatory, and antioxidant effects [21] .…”

“…BBH exhibits broad‐spectrum antibacterial activity against various Gram‐negative and Gram‐positive bacteria, [22] particularly demonstrating pronounced inhibitory effects on Staphylococcus aureus, Streptococcus hemolyticus, Vibrio cholera, Salmonella typhi and Corynebacterium diphtheriae . However, it faces challenges in terms of solubility, stability, and bioavailability, which limit its application in the field of biomedical research [20] …”

“…However, it faces challenges in terms of solubility, stability, and bioavailability, which limit its application in the field of biomedical research. [20] However, metal ions such as Zn 2 + , Co 2 + , Cu 2 + and Ag + released along with the degradation of MOFs have the advantages of low drug resistance and broad antibacterial spectrum, which are considered as effective substitutes for antibiotics. [23] Compared with monometallic MOFs, bimetallic MOFs with two metal centers have enhanced synergistic antibacterial effects.…”