In situ gelling-polypeptide hydrogel systems for the subcutaneous transplantation of MIN6 cells

Lin, Hsiu-Chao; Chen, Chen-Yi; Kao, Chen-Wei; Wu, Shuting; Chen, Chen-Ling; Shen, Chia-Rui; Juang, Jyuhn‐Huarng; Chu, I‐Ming

doi:10.1007/s10965-020-2032-x

Cited by 7 publications

(20 citation statements)

References 36 publications

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“…After 29 days, the graft became hyperintense on MR scans because insulinoma-derived MIN6 cells proliferated to generate daughter cells which did not contain CSPIO nanoparticles. Meanwhile, the hyperintense MR images of the implanted hydrogels without MIN6 cells gradually declined as a result of degradation of the materials [ 27 ]. Although subcutaneous transplantation of MIN6 cells in mice has been investigated [ 24 , 35 ], to the best of our knowledge, we are the first to use MRI for the graft detection and monitoring.…”

Section: Resultsmentioning

confidence: 99%

“…The former ( Figure 5 B, 0.3 × 0.2 × 0.1 cm) were much bigger than the latter ( Figure 5 A, 1.3 × 1 × 0.6 cm), although both were well vascularized at day 65. Since the mPEG-poly(Ala) hydrogels are biodegradable [ 27 ], their graft size decreased with time. This also explains the gradual decline of hyperintense MR images of the implanted hydrogels ( Figure 3 ).…”

Section: Resultsmentioning

confidence: 99%

“…As shown in Figure 5 , the graft was positive for insulin ( Figure 5 D), iron ( Figure 5 E), and CD31. However, compared to 2-week grafts [ 27 ], the hydrogel was barely seen. In addition, MIN6 cells were arranged into clusters and confluence.…”

Section: Resultsmentioning

confidence: 99%

“…The mPEG-poly(Ala) diblock copolymer was prepared via the ROP of l -alanine- N -carboxyanhydride (NCA-Ala), with mPEG-NH 2 serving as the macroinitiator [ 27 ]. Briefly, mPEG-NH 2 was dissolved in 15 mL of toluene, and azeotropic distillation was used to obtain a final volume of approximately 5 mL to remove residual water.…”

Section: Methodsmentioning

confidence: 99%

“…They enable in situ gelling upon being injected into appropriate sites in the body [ 26 ]. For instance, we recently demonstrated the feasibility of using poly(ethylene glycol) methyl ether (mPEG)-poly(Ala) hydrogels as delivery carriers for subcutaneous transplantation of MIN6 cells [ 27 ].…”

Section: Introductionmentioning

confidence: 99%

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Noninvasive Tracking of mPEG-poly(Ala) Hydrogel-Embedded MIN6 Cells after Subcutaneous Transplantation in Mice

et al. 2021

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Recently, we demonstrated the feasibility of subcutaneous transplantation of MIN6 cells embedded in a scaffold with poly(ethylene glycol) methyl ether (mPEG)-poly(Ala) hydrogels. In this study, we further tracked these grafts using magnetic resonance (MR) and bioluminescence imaging. After being incubated overnight with chitosan-coated superparamagnetic iron oxide (CSPIO) nanoparticles and then mixed with mPEG-poly(Ala) hydrogels, MIN6 cells appeared as dark spots on MR scans. For in vivo experiments, we transfected MIN6 cells with luciferase and/or incubated them overnight with CSPIO overnight; 5 × 106 MIN6 cells embedded in mPEG-poly(Ala) hydrogels were transplanted into the subcutaneous space of each nude mouse. The graft of CSPIO-labeled MIN6 cells was visualized as a distinct hypointense area on MR images located at the implantation site before day 21. However, this area became hyperintense on MR scans for up to 64 days. In addition, positive bioluminescence images were also observed for up to 64 days after transplantation. The histology of removed grafts showed positive insulin and iron staining. These results indicate mPEG-poly(Ala) is a suitable scaffold for β-cell encapsulation and transplantation. Moreover, MR and bioluminescence imaging are useful noninvasive tools for detecting and monitoring mPEG-poly(Ala) hydrogel-embedded MIN6 cells at a subcutaneous site.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Noninvasive Tracking of mPEG-poly(Ala) Hydrogel-Embedded MIN6 Cells after Subcutaneous Transplantation in Mice

et al. 2021

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Self‐assembly and sol‐to‐gel transition of thermosensitive methoxy‐polyethylene glycol‐polyalanine block copolymer hydrogels

Chu

2020

J of Applied Polymer Sci

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Certain amphiphilic polymers can self-assemble to form various structures, such as micelles and hydrogels in aqueous medium, depending on the concentration or temperature. These different structures have found many applications in biomedical and material processing fields. The assembly processes of the polypeptide-containing diblock copolymer, methoxy poly(ethylene glycol)block-poly(L-alanine) (mPEG-PA), was studied to elucidate the relationship between different structures with parameters such as chain lengths (volume fraction) of the two blocks, molecular weights, medium polarity, temperature, and concentration. Gelation mechanism was especially focused in the study. Various solution-cast morphologies observed by scanning electron microscopy (SEM) imaging including cylinders and micelles aggregates can be obtained by using solvents with different polarity for casting with simply tuning the chain lengths. The sol-to-gel transition mechanism can be best explained by phase separation between polymer-rich and water-rich phases. It might be spinodal decomposition process to cause the phase separation. Furthermore, the branch and flack structures can be observed due to phase separation mechanism and different chain lengths by SEM images. This result offers new insights into behavior of mPEG-PA system, enabling more controlled manipulation to various applications.

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Mimicry of silk utilizing synthetic polypeptides

Chan

Lentz

Gurr

et al. 2022

Progress in Polymer Science

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In situ gelling-polypeptide hydrogel systems for the subcutaneous transplantation of MIN6 cells

Cited by 7 publications

References 36 publications

Noninvasive Tracking of mPEG-poly(Ala) Hydrogel-Embedded MIN6 Cells after Subcutaneous Transplantation in Mice

Noninvasive Tracking of mPEG-poly(Ala) Hydrogel-Embedded MIN6 Cells after Subcutaneous Transplantation in Mice

Self‐assembly and sol‐to‐gel transition of thermosensitive methoxy‐polyethylene glycol‐polyalanine block copolymer hydrogels

Mimicry of silk utilizing synthetic polypeptides

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