In situ generation of electrochemiluminescent DNA nanoflowers as a signal tag for mucin 1 detection based on a strategy of target and mimic target synchronous cycling amplification

Li, Sheng-Kai; Chen, Anyi; Niu, Xiaoxue; Liu, Zhiting; Du, Miao; Yuan, Ruo; Zhuo, Ying

doi:10.1039/c7cc04747a

Cited by 31 publications

(19 citation statements)

References 34 publications

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“…Hence, it is particularly significant to enhance the signal probe immobilization capacity on the substrate and avoid the inner filter effect. Recently, the insertion and labeling of luminophore on the DNA nanomaterials − have been widely investigated, due to their favorable biocompatibility, excellent controllability, and easy editability. Nevertheless, the preparation of those luminous materials suffered from the complicated design of DNA, unstable embedding of luminophore, and finite quantity of luminous probes, leading to the unstable and low ECL signal.…”

mentioning

confidence: 99%

Three-Dimensional Cadmium Telluride Quantum Dots–DNA Nanoreticulation as a Highly Efficient Electrochemiluminescent Emitter for Ultrasensitive Detection of MicroRNA from Cancer Cells

et al. 2019

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In this work, a novel three-dimensional cadmium telluride quantum dots–DNA nanoreticulation (3D CdTe QDs–DNA-NR) was used as a signal probe with the dual-legged DNA walker circular amplification as target conversion strategy to establish a pioneering electrochemiluminescence (ECL) biosensing strategy for ultrasensitive detection of microRNA-21 form cancer cells. Herein, such a 3D luminous nanomaterial with reticular structure not only supported abundant CdTe QDs to avoid the inner filter effect for obtaining a high ECL efficiency but also contained the hemin/G-quadruplex as coreaction accelerator in the 3D CdTe QDs–DNA-NR/S2O8 2– system for the enhancement of ECL intensity. Furthermore, with the target-induced dual-legged DNA walker circular amplification strategy, a mass of output DNA was produced to connect with the 3D CdTe QDs–DNA-NR for the construction of the ECL biosensor, which realized the ultrasensitive detection of microRNA-21 from 100 aM to 100 pM and the detection limit down to 34 aM. Significantly, this work could be readily extended for the detection of other biomolecules to provide a neoteric channel for disease diagnosis.

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confidence: 99%

Three-Dimensional Cadmium Telluride Quantum Dots–DNA Nanoreticulation as a Highly Efficient Electrochemiluminescent Emitter for Ultrasensitive Detection of MicroRNA from Cancer Cells

et al. 2019

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“…The large number of repetitive sequences in the DNFs provide abundant binding sites, effectively improving the sensitivity of detection. Zhuo and co‐workers used DNFs as electro‐chemiluminescent (ECL) signal tags in an ultrasensitive sensor to detect a tumor biomarker, mucin 1 (MUC1) protein, which is overexpressed in malignant tumors [24] . Three cycling amplification strategies were integrated on a sensing interface, including two exonuclease‐mediated target recycling amplification processes and a mimic target‐triggered RCA reaction.…”

Section: Applicationsmentioning

confidence: 99%

Programmable DNA Nanoflowers for Biosensing, Bioimaging, and Therapeutics

Dong

et al. 2020

Chemistry A European J

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DNA nanostructures have shown excellent prospects in biomedical applicationso wing to their unique sequence programmability,f unction designability,a nd biocompatibility.A satype of uniqueD NA-inorganic hybrid nanostructures, DNA nanoflowers (DNFs)h ave attracted considerable attention in the past few years. Precise design of the DNA sequence enablest he functions of DNFs to be customized. Specifically,D NFse xhibit high physiological stability and more diversep roperties by virtue of the incorporation of inorganic materials, which in turn have been applied in an assortment of biomedical fields. In this review,t he design, synthesis, and biomedical applications of program-mable DNFs are discussed. First, the background of DNAbased materialsa nd the fundamentals of DNFs are briefly introduced. In the second part, two synthetic methods of DNFs are categorized as the rolling circle amplification and salt aging method, focusing on the formation mechanism of DNFs and differences between the synthetic methods. In the third part, the biomedical applicationso fD NFs functional materialsa re summarized, includingb iosensing, bioimaging, and therapeutics. Finally,t he challenges and future opportunities of DNFsa re discussed towardm ore widespreada pplications.

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“…For instance, fluorescent dye‐conjugated DNA was modified on the Co@G surfaces through π–π stacking interactions for specific detection of DNA molecules . Aptamers, single‐stranded DNA or RNA selected using the systematic evolution of ligands by exponential enrichment (SELEX) technique can bind to specific target molecules with high affinity and show much better stability than antibodies . Based on the superior merits of aptamers, various types of aptamers were modified on the surface of MGNs for accurate Raman and TPL imaging application of specific cells or tissues .…”

Section: Surface Functionalization Of Graphitic Nanocapsulesmentioning

confidence: 99%

Recent Advances in Multifunctional Graphitic Nanocapsules for Raman Detection, Imaging, and Therapy

Liu

Zhu

et al. 2019

Small Methods

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To date, a variety of graphitic nanomaterial-based applications have facilitated the development of biomedical applications, such as carbon quantumdot-based biosensors and imaging systems, [14][15][16][17] graphene oxide-based biosensors [18][19][20][21][22] and drug delivery systems, [20,[23][24][25] and carbon nanotube-based therapy applications. [26][27][28][29] Compared with other widely used nanomaterials, graphitic nanomaterials show superior fluorescence quenching performance, larger Raman scattering cross-sections than conventional organic Raman tags, [30] and better biocompatibility. [31] With the goal of broadening their applications, great attention has been focused on integrating graphitic materials with other inorganic nanomaterials such as noble metals [32,33] and magnetic nanomaterials. [34,35] Such graphitic nanomaterial-metal hybrid nanostructures make full use of the excellent properties of both graphitic and metal nanomaterials, and have much wider application prospect in bioanalysis. [36] However, most of the reported hybrid nanostructures, in which metal nanomaterials are exposed on the surface of graphitic nanomaterials encounter difficulty in maintaining their morphologies and stable properties under harsh conditions, such as long-term laser irradiation or strong acidic environments, which might cause inaccurate bioanalytical results and even potential biotoxicity. [37][38][39] Therefore, it is critical to prepare novel graphitic nanomaterials with superstability to meet the demands of reliable bioanalysis and biomedicine.As a type of novel graphite nanomaterial with a core-shell structure, superior physicochemical properties, good biocompatibility, superior stability, and controllable size, graphitic nanocapsules have been extensively applied in biosensing, [40,41] bioimaging, [42,43] drug delivery, [43,44] and cancer treatment. [45] Benefiting from the large surface area of the outside graphitic shell, graphitic nanocapsules supply superior nanoplatforms for drugs and targeting-molecule loading, which further broadens their biomedical applications. [42,44,46] In addition, the graphitic shell of graphitic nanocapsules possesses several unique Raman scattering bands and can act as a Raman signal tag or stable internal standard (IS) for accurate surface enhanced Raman scattering (SERS) analysis. [46,47] Particularly, the 2D band (≈2650 cm −1 ) within the cellular Raman-silent region is beneficial to avoiding the interference signals from Benefiting from their unique physicochemical properties, graphitic nanocapsules with a core-shell structure have attracted tremendous interest in bioanalysis and biomedicine recently. Using rational design, various types of graphitic nanocapsules with controllable properties are prepared to meet the demands of different biomedical applications. Graphitic nanocapsules exhibit excellent stability and superior capacity for loading nucleic acids, proteins, small molecules, and drugs due to the large specific surface area of their graphitic shells. Moreover, usin...

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In situ generation of electrochemiluminescent DNA nanoflowers as a signal tag for mucin 1 detection based on a strategy of target and mimic target synchronous cycling amplification

Cited by 31 publications

References 34 publications

Three-Dimensional Cadmium Telluride Quantum Dots–DNA Nanoreticulation as a Highly Efficient Electrochemiluminescent Emitter for Ultrasensitive Detection of MicroRNA from Cancer Cells

Three-Dimensional Cadmium Telluride Quantum Dots–DNA Nanoreticulation as a Highly Efficient Electrochemiluminescent Emitter for Ultrasensitive Detection of MicroRNA from Cancer Cells

Programmable DNA Nanoflowers for Biosensing, Bioimaging, and Therapeutics

Recent Advances in Multifunctional Graphitic Nanocapsules for Raman Detection, Imaging, and Therapy

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