In situ green synthesis of Cu-doped ZnO based polymers nanocomposite with studying antimicrobial, antioxidant and anti-inflammatory activities

Abstract

The use of eco-friendly methods for the synthesis of nanoparticles and its nano-composite has become a public demand nowadays to reduce the risks of chemical methods. In the current study, green synthesis of Cu-doped ZnO based polymers nan-ocomposite was performed. Various instrumental analysis including UV–vis, ATR-FTIR spectroscopy, XRD, SEM coupled with energy dispersive X-ray analysis, TEM and Thermal gravimetric were used to characterize nano-composite. Highly antibacterial activity of the synthesized nano-composite was recorded against tested microorganisms with promising efficacy against bacteria namely; Bacillus subtilis, Staphylococcus aureus, Enterococcus faecalis, Proteus vulgaris, Pseudomonas aeruginosa, Escherichia coli, Salmonella typhimurium and yeast (Candida albicans) but unfortunately not against black fungus (Mucor circinelloides) and filamentous fungi Aspergillus flavus and A. niger. Anti-inflammatory of nano-composite represented by hemolysis inhibition was observed at using low concentration (100 µg/mL) with enhancing 23.85% compared with free nano-composite while at high concentrations 500 and1000 µg/mL the anti-inflammatory activity was approximately similar with enhancing 3.91% and 1.99%, respectively. Antioxidant of the nano-composite was better than the antioxidant of free nano-composite at all tested concentrations, moreover the IC50 of the nano-composite (91.16 µg/mL) was less than the IC50, (203.65 µg/mL) of the free nano-composite.

Phytochemical Characterization and Efficacy of Artemisia judaica Extract Loaded Chitosan Nanoparticles as Inhibitors of Cancer Proliferation and Microbial Growth

“…Then, the metabolic product of MTT was resuspended in 200 µL of DMSO and left for 5 min on the shaking table at 150 rpm to enhance the mixture of the metabolic product with the solvent. At 560 nm and a subtracted background at 620 nm, the optical density was read to determine the viability of the treated cells [ 2 , 26 ]. Cell cytopathic effects were monitored using an inverted microscope.…”

“… Well-diffusion assays to assess the [ 1 ] activity of chitosan, [ 2 ] positive controls (antibiotic/antifungal), [ 3 ] Artemisia judaica extract, and [ 4 ] chitosan nanoparticles (CNPs) loaded extract against pathogenic bacterial and fungal isolates. …”

“…Plants are a rich source for diverse chemical compounds with various therapeutic properties that can be used alone or in combination with other medicines to treat infectious and non- infectious diseases [ 1 , 2 , 3 ]. Although numerous plant-derived products have been discovered and clinically used, this source still offers high probability for the discovery of new and promising therapeutic compounds for treating human illness.…”

Effectiveness of oil-based nanoemulsions with molecular docking of its antimicrobial potential

“…Nanoparticles are used in various fields including medicinal, pharmacological, agricultural, chemical, and electrical. Nano-form materials have the advantages of ratio of size to shape, more surface energy, and high surface area (Abdelghany et al 2018;Ganash et al 2018;Al-Rajhi et al 2022a). The properties of nanomaterials (NMs) enable them to penetrate easily inside the materials, which can lead to rapid dissolution and spreading on the materials surface, as well as faster interaction with materials.…”

Molecular Docking and Efficacy of Aloe vera Gel Based on Chitosan Nanoparticles against Helicobacter pylori and Its Antioxidant and Anti-Inflammatory Activities

“…Nanoparticles (NPs) have played and still play an important role in many medical, industrial, agricultural, environmental, and food fields [ 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 ]. From the applied NPs, chitosan nanoparticles (CSNPs) have been lately used at large scales for medical applications such as drug carriers and cosmetics [ 24 ].…”