In situ hybrid nano drug delivery system (IHN-DDS) of antiretroviral drug for simultaneous targeting to multiple viral reservoirs: An in vivo proof of concept

Jindal, Anil; Bachhav, Sagar S.; Devarajan, Padma V.

doi:10.1016/j.ijpharm.2017.02.024

Cited by 35 publications

(21 citation statements)

References 39 publications

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“…Therefore, there is urgent need for improving diagnostic means for early detection and for researching more selective drug release systems with few side effects for treatment. Compared with traditional cancer treatment, the development of nano-targeted drug release systems has led to a new strategy because cellular interaction and communication are often at the nano scale [ 4 , 5 ]. The foremost advantages of employing nano drug delivery systems include specific delivery for targeted action, which can overcome the barrier of drug penetration, and enhancement of the bioavailability and therapeutic performance of antitumor drugs.…”

Section: Introductionmentioning

confidence: 99%

Design and Evaluation of Europium Containing Mesoporous Bioactive Glass Nanospheres: Doxorubicin Release Kinetics and Inhibitory Effect on Osteosarcoma MG 63 Cells

Zhang

Wang

et al. 2018

Nanomaterials

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Functional ions and drug factors play a vital role in stimulating bone tissue regeneration as we understand it. In this work, europium-containing mesoporous bioactive glass nanospheres (Eu/MBGs), composed of 60% SiO2—(36–x)%CaO—x%Eu2O3—4%P2O5 (x = 0, 0.5, 1, 2 mol%), were prepared by a facile sol-gel process. The results indicate that Eu ions play an important role to influence the microstructure of MBGs, in which a suitable concentration of Eu (1 mol%) increases their surface area (502 m2/g) as well as their pore volume (0.34 cm3/g). Proper doping of Eu ions in MBGs can observably induce apatite mineralization and improve the doxorubicin (DOX) release behavior. Furthermore, DOX-loaded Eu/MBGs could maintain a long-term inhibitory effect on the viability of osteosarcoma MG 63 cells. This work has demonstrated that it is possible to develop functional Eu/MBGs by combining excellent apatite-mineralization ability, controllable drug (DOX) release and antitumor functions for the therapy of bone tissue regeneration.

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Section: Introductionmentioning

confidence: 99%

Design and Evaluation of Europium Containing Mesoporous Bioactive Glass Nanospheres: Doxorubicin Release Kinetics and Inhibitory Effect on Osteosarcoma MG 63 Cells

Zhang

Wang

et al. 2018

Nanomaterials

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“…In contrast, in situ nanotechnology presents a method wherein generation of NPs is achieved by simple addition of a monophasic preconcentrate comprising drug, carrier (polymer/lipid), and stabilizers to an aqueous phase. Our group has reported in situ nanotechnology as a facile method for instantaneous and on‐site generation of NPs to enable point of care application . A distinct advantage of in situ technology is total bypass of the technological hurdles and stability issues associated with conventional NP fabrication methods.…”

Section: Introductionmentioning

confidence: 99%

“…An in situ hybrid nanoparticulate drug delivery system of nevirapine designed for simultaneous targeting of multiple reservoirs in HIV exhibited >90% drug entrapment in a mixture of solid lipid NPs and micelles which was instantaneously generated when the preconcentrate was diluted with isotonic dextrose injection. Following intravenous administration in Sprague Dawley rats, the heterogenous nanosystem revealed high drug accumulation in the RES organs of liver and spleen and also the brain, which represents a remote HIV reservoir …”

Section: Introductionmentioning

confidence: 99%

“…Our group has reported in situ nanotechnology as a facile method for instantaneous and on-site generation of NPs to enable point of care application. [20][21][22] A distinct advantage of in situ technology is total bypass of the technological hurdles and stability issues associated with conventional NP fabrication methods. First generation in situ nanotechnology relies on dilution of monophasic preconcentrates with an aqueous media like potable drinking water just prior to oral administration or dextrose/ dextrose saline injection just prior to intravenous injection.…”

Section: Introductionmentioning

confidence: 99%

“…Following intravenous administration in Sprague Dawley rats, the heterogenous nanosystem revealed high drug accumulation in the RES organs of liver and spleen and also the brain, which represents a remote HIV reservoir. 21 Second generation in situ nanotechnology can additionally enable passive targeting through stealth or active targeting enabled by inclusion of a receptor ligand. Herein, the aqueous phase is provided along with the preconcentrate and comprises the stealth agent or receptor ligand.…”

Section: Introductionmentioning

confidence: 99%

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Polymeric curcumin nanoparticles by a facile in situ method for macrophage targeted delivery

Jahagirdar

Gupta

Kulkarni

et al. 2018

Bioengineering & Transla Med

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Targeting macrophages is a promising strategy for improved therapy of intracellular infections as macrophages exhibit rapid phagocytosis of particles >200 nm. Entrapment of Curcumin (CUR) in nanocarriers could provide bioenhancement and macrophage targeting. We present a simple and facile in situ nanoprecipitation approach for instantaneous and on-site generation of curcumin nanoparticles (ISCurNP). ISCurNP optimised by Box-Behnken design exhibited average size of 208.25 AE 7.55 nm and entrapment efficiency of 90.16 AE 1.17%. Differential scanning calorimetry and X-Ray diffraction confirmed amorphization of CUR in ISCurNP. Sustained release was observed over 72 hr in vitro at lysosomal pH 4.5. Rapid and high uptake in RAW 264.7 macrophages was confirmed by flow cytometry and high performance liquid chromatography. Confocal microscopy established localisation of ISCurNP in lysosomal compartment. The facile in situ nanoprecipitation method provides simple, scalable technology to enable macrophage targeted delivery of CUR, with great promise for improved therapy of intracellular infections. K E Y W O R D SCurcumin, in situ, nanoparticles, macrophages, nanoprecipitation, intracellular uptake, Box Behnken 1 | INTRODUCTION Curcumin (CUR), a natural polyphenol has generated tremendous excitement as a nutraceutical due to its multifarious pharmacological activities.1,2 The anticancer 3-6 and anti-inflammatory 7,8 properties of CUR have been widely explored. CUR, an established anti-infective for eons, is currently being extensively researched for antibacterial including antimycobacterial, antiviral, and antifungal activity. 9-11Despite the excellent therapeutic potential, a considerable impediment in exploitation of CUR as a therapeutic agent is its hydrophobic nature and consequently poor aqueous solubility. 12Nanonization of particles has proven to be a competent approach to enhance solubility and bioavailability of hydrophobic poorly soluble drugs. 13,14 Furthermore, such drugs when entrapped in nanocarriers with size titrated in the range of 200-600 nm can aid targeting to the reticuloendothelial system (RES) organs and cells, especially macrophages, the primary site of various intracellular infections. 15-18Macrophages constitute defense system of the body and exhibit rapid phagocytosis of particles >200 nm. | ISCurNPPreconcentrates were added to filtered distilled water for instantaneous formation ISCurNP. | Optimization of ISCurNP 2.4.1 | Preliminary screeningPreliminary screening was carried out using the one variable at a time | Design of experiment: Box-Behnken designISCurNP was optimized by applying Box-Behnken design. Independent variables selected were concentration of Soluplus ® (X1), concentration of PLGA (X2), and volume of DMA (X3) in the preconcentrate and the dependent variables (responses) were particle size (PS; Y1), and percent entrapment efficiency (%EE; Y2) of the generated ISCurNP. Curcumin concentration (10 mg) and aqueous dilution volume (10 ml) were maintained constant. The indepen...

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Application of Plant‐Based Nanobiopesticides That Could Be Applied for the Rejuvenation of Heavily Contaminated Environments

Adetuyi,

Olajide,

Adetunji

2024

Handbook of Agricultural Biotechnology

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In situ hybrid nano drug delivery system (IHN-DDS) of antiretroviral drug for simultaneous targeting to multiple viral reservoirs: An in vivo proof of concept

Cited by 35 publications

References 39 publications

Design and Evaluation of Europium Containing Mesoporous Bioactive Glass Nanospheres: Doxorubicin Release Kinetics and Inhibitory Effect on Osteosarcoma MG 63 Cells

Design and Evaluation of Europium Containing Mesoporous Bioactive Glass Nanospheres: Doxorubicin Release Kinetics and Inhibitory Effect on Osteosarcoma MG 63 Cells

Polymeric curcumin nanoparticles by a facile in situ method for macrophage targeted delivery

Application of Plant‐Based Nanobiopesticides That Could Be Applied for the Rejuvenation of Heavily Contaminated Environments

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