In situ hybridization shows distinct patterns of mucin gene expression in normal, benign, and malignant pancreas tissues

Balagué, Cristina; Audie, J P; Porchet, Nicole; Real, Francisco X.

doi:10.1016/0016-5085(95)90406-9

Cited by 136 publications

(109 citation statements)

References 23 publications

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“…Similar distinct cell-specific apomucin expression corresponding to each histological element reported in other organs such as the pancreas and stomach. [16][17][18][19] We confirmed in this study that the mRNA expressions of MUC2, MUC3, MUC5, and MUC6 apomucins resembled each protein expression in both hepatolithiasis and control livers. MUC1 mRNA expression was widespread in large bile ducts in both hepatolithiasis and control livers, whereas MUC1 protein was expressed focally in large bile ducts in hepatolithiasis.…”

Section: Discussionsupporting

confidence: 73%

“…Such changes in mucin gene expression in benign proliferative lesions have been shown in pancreatic ducts. 19 In conclusion, the increased expression of MUC5 and MUC6 apomucin in hepatolithiasis revealed in this study could contribute to changing the properties of biliary mucin itself and, thereby, could contribute to the pathogenesis and development of hepatolithiasis.…”

Section: Discussionmentioning

confidence: 56%

“…There is growing evidence that mucins are involved in diverse and important biological roles, such as those of receptors for bacteria or viruses, cell-to-cell or cell-to-substratum interactions, and modulation of immune responses. [16][17][18][19] Thus, there may be crucial differences in the bile caused by proportion of MUC3, MUC5, and MUC6 apomucins regarding lithogenesis. They may include the binding capacity with calcium bilirubinate and cholesterol, crystallization of cholesterol, and interaction with factors precipitating lithogenesis, such as calcium-binding protein.…”

Section: Discussionmentioning

confidence: 99%

“…1 To date, eight apomucins (MUC 1-7 and MUC5B) have been identified 2-8 and the tissue-and cell-specific expression of these mucin genes is now emerging, suggesting that each has a distinct role. [9][10][11][12][13][14][15][16][17][18][19] …”

mentioning

confidence: 99%

“…1 To date, eight apomucins (MUC 1-7 and MUC5B) have been identified [2][3][4][5][6][7][8] and the tissue-and cell-specific expression of these mucin genes is now emerging, suggesting that each has a distinct role. [9][10][11][12][13][14][15][16][17][18][19] There are only a few reports describing the expression of apomucins in the normal and pathologic livers. [13][14][15] We reported the frequent and homogeneous expression of MUC3 in normal intrahepatic large bile ducts, but not of MUC1, MUC2, and MUC5/6 in normal adult liver.…”

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confidence: 99%

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Expression of apomucins in the intrahepatic biliary tree in hepatolithiasis differs from that in normal liver and extrahepatic biliary obstruction

1998

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Mucin plays an important role in the development of hepatoliths, which are formed within the intrahepatic large bile ducts. To date, eight apomucins, components of mucin, have been identified. The purpose of this study is to characterize the expression of MUC1, MUC2, MUC3, MUC5, and MUC6 apomucins at both the protein and messenger RNA (mRNA) levels in the intrahepatic large bile ducts and peribiliary glands in 36 livers from patients with hepatolithiasis, in 25 livers from the patients with extrahepatic biliary obstruction, and in 23 histologically-normal livers. MUC3 and MUC6 were constantly and focally expressed, respectively, in the biliary epithelial cells in the intrahepatic large bile ducts in normal livers, extrahepatic biliary obstruction, and, also, hepatolithiasis. In hepatolithiasis, MUC1, MUC2, and MUC5 apomucins were also focally expressed in 33%, 64%, and 89%, respectively, of the large intrahepatic bile ducts examined, whereas such expression was infrequent in normal livers and in extrahepatic biliary obstruction. The markedly proliferated intramural and extramural peribiliary glands in hepatolithiasis frequently expressed MUC3 and MUC6 apomucins and focally expressed MUC2 and MUC5 apomucins. All of the apomucins mRNA expression, except that of MUC1, resembled each protein expression. In conclusion, the characteristics of apomucins in the intrahepatic biliary tree in hepatolithiasis differs from those in normal livers and extrahepatic biliary obstructrion. Increased expression of gel-forming mucins, such as MUC5 and MUC2, could be involved in the formation and development of hepatolithiasis. (HEPATOLOGY 1998;27:46-53.)Epithelial mucins and their alterations are involved in the pathogenesis of neoplastic and non-neoplastic diseases. These mucins have in common a protein backbone which is generally called apomucin or mucin core protein. 1 To date, eight apomucins (MUC 1-7 and MUC5B) have been identified 2-8 and the tissue-and cell-specific expression of these mucin genes is now emerging, suggesting that each has a distinct role. [9][10][11][12][13][14][15][16][17][18][19]

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Section: Discussionsupporting

confidence: 73%

Section: Discussionmentioning

confidence: 56%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Expression of apomucins in the intrahepatic biliary tree in hepatolithiasis differs from that in normal liver and extrahepatic biliary obstruction

1998

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The MUC6 secretory mucin gene is expressed in a wide variety of epithelial tissues

et al. 1998

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Secretory mucins play an important role in the cytoprotection of epithelial surfaces and are used as tumour markers in a variety of cancers. The MUC6 secretory mucin was originally isolated from a gastric cDNA library. The aim was to determine the specific type and location of MUC6 mucin gene expression in a wide range of human adult and fetal epithelial tissues. In situ hybridization, RNA analysis, and immunohistochemistry were used to quantify and localize mucin gene expression. The data obtained show that MUC6 is highly expressed in gastric mucosa, duodenal Brunner's glands, gall bladder, seminal vesicle, pancreatic centroacinar cells and ducts, and periductal glands of the common bile duct; focal expression is seen in basal endometrial and endocervical glands. MUC6 epitopes were also highly expressed in 7/10 pancreatic cancers and 7/10 cholangiocarcinomas and focally expressed in 4/10 endocervical adenocarcinomas. Expression of MUC6 occurs early in fetal development and was observed in Brunner's glands and pancreatic ducts at 18–19 weeks and in gastric glands at 20 weeks' gestation. The tissue distribution of the MUC6 secretory mucin indicates that it may function to protect epithelial tissues from a wide range of substances. Expression of MUC6 is frequently preserved in pancreatic and bile duct adenocarcinomas, but it is only sparsely expressed in endocervical carcinomas. Copyright © 1998 John Wiley & Sons, Ltd.

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Expression of MUC1 and MUC2 mucin core proteins and their messenger RNA in gall bladder carcinoma: an immunohistochemical andin situ hybridization study

et al. 1999

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Expression of mucin core protein MUC1 and MUC2 was examined at the protein and mRNA level in 55 cases of carcinoma and 20 of dysplasia, and in 15 non‐dysplastic epithelia of the gall bladder. In non‐dysplastic epithelium, MUC1 protein was not expressed, while in dysplasia, MUC1 was focally expressed in ten cases, particularly in those associated with carcinoma. In carcinoma, MUC1 was expressed heterogeneously, and the frequency and extent of MUC1 expression increased with histological dedifferentiation. MUC1 was found on the apical cell surface and also in the cytoplasm in well‐ and moderately‐differentiated carcinoma, and on the cell border in poorly‐differentiated cases. In infiltrative regions, MUC1 expression was more predominant and MUC1 frequently leaked outside the foci of carcinoma. By contrast, MUC2 was focally expressed in non‐dysplastic as well as in dysplastic epithelia and more frequently in well‐differentiated adenocarcinoma. MUC2‐positive cells resembled goblet cells, whether in non‐dysplastic epithelium, dysplasia or carcinoma. Cell proliferative activity was higher in MUC1‐positive than in MUC1‐negative carcinoma cells. Distributions of MUC1 and MUC2 mRNA signals and of MUC1 and MUC2 proteins were similar in carcinoma and dysplasia. These results suggest that MUC1 expression by gall bladder carcinoma may reflect histological dedifferentiation, increased proliferative activity, and invasiveness, while MUC2 expression is related to lower proliferative activity and reflects some differentiation towards goblet cells; and that MUC1 expression in gall bladder dysplasia reflects malignant transformation. Copyright © 1999 John Wiley & Sons, Ltd.

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In situ hybridization shows distinct patterns of mucin gene expression in normal, benign, and malignant pancreas tissues

Cited by 136 publications

References 23 publications

Expression of apomucins in the intrahepatic biliary tree in hepatolithiasis differs from that in normal liver and extrahepatic biliary obstruction

Expression of apomucins in the intrahepatic biliary tree in hepatolithiasis differs from that in normal liver and extrahepatic biliary obstruction

The MUC6 secretory mucin gene is expressed in a wide variety of epithelial tissues

Expression of MUC1 and MUC2 mucin core proteins and their messenger RNA in gall bladder carcinoma: an immunohistochemical andin situ hybridization study

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