In-situ imaging of Langmuir monolayers by second-harmonic microscopy

Flörsheimer, M.; Looser, Herbert; Küpfer, M.; Günter, Peter

doi:10.1016/s0040-6090(95)08415-0

Cited by 19 publications

(12 citation statements)

References 12 publications

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“…IJK are the tensorial components of the macroscopic nonlinear susceptibility χ (2) with I, J, K in the (X, Y, Z) frame (Fig. 1a), and:…”

Section: A Reduction Of the Molecular Orientational 3d Distribution mentioning

confidence: 99%

“…In the case of collagen, a C 6 symmetry is assumed from the known crystalline structure of this macromolecule [3]. Denoting 3 the high symmetry axis of the C 6 symmetry cylinder and (1, 2) its perpendicular plane, the collagen symmetry is therefore reduced to two microscopic tensorial coefficients under non-resonant excitation conditions: χ (2) 333 and χ (2) 311 = χ (2) 322 (with all index permutation permitted in case of valid Kleinman symmetry conditions). The ratio χ (2) 333 /χ (2) 311 , often used to characterize molecular order in collagen, has been found to range between 0.8 and 2.6 [3,16,17,[19][20][21].…”

Section: B Relation Between Order Parameters and Microscopic Tensorimentioning

confidence: 99%

“…Second Harmonic Generation (SHG) microscopy, since its first development [1][2][3] and its introduction in bio-imaging [4][5][6], is now widely used to image ordered bio-molecular assemblies in complex samples. Coherent SHG occurring naturally in non-centrosymmetric structures such as collagen [3], skeletal muscles [4] and microtubules [6], is today exploited as a functional contrast [7,8], possibly in conjunction with other nonlinear optical signatures [9], with the ultimate goal of developing diagnostics of pathological effects related to tissues and cell architecture.…”

Section: Introductionmentioning

confidence: 99%

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Generic model of the molecular orientational distribution probed by polarization-resolved second-harmonic generation

et al. 2012

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International audienceIn this work we investigate a generic method able to extract information on molecular organization in biological samples from polarized second harmonic generation (SHG) microscopy, without the need to infer an a priori model for the molecular orientational distribution. The mean orientation of this distribution, as well as its first and third orders of symmetry, are estimated by monitoring SHG intensity signals under a varying incident polarization. We introduce, in particular, a reduction of the problem to a two-dimensional approach appropriate to the microscopy geometry. This method allows us to retrieve determining information which is not available in the traditional model-oriented methods, as illustrated in molecular-order imaging in collagen fibrils. The precision of the parameters estimation is evaluated by a Monte Carlo analysis, based on the Poisson noise statistics of the measured signal

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“…IJK are the tensorial components of the macroscopic nonlinear susceptibility χ (2) with I, J, K in the (X, Y, Z) frame (Fig. 1a), and:…”

Section: A Reduction Of the Molecular Orientational 3d Distribution mentioning

confidence: 99%

Section: B Relation Between Order Parameters and Microscopic Tensorimentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Generic model of the molecular orientational distribution probed by polarization-resolved second-harmonic generation

et al. 2012

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“…[16][17][18] In contrast to linear optical techniques such as ellipsometric, polarization, and Brewster angle microscopy, SHM has an additional advantage of being able to distinguish between a parallel (polar) and an antiparallel (axial, centrosymmetric) order of molecules at the interface (surface) plane. Recently, SHM was applied for the observation of Langmuir monolayers at the air/water interface.…”

Section: Second Harmonic Microscopy (Shm)mentioning

confidence: 99%

Recent Progress in Analytical SHG Spectroscopy

Yamada

Lee

1998

ANAL. SCI.

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The progress of laser technology has brought extensive applications of nonlinear optical (NLO) spectroscopy to analytical fields. Especially, optical second harmonic generation (SHG), which is one of the most popular NLO phenomena, has been successfully applied to the study of interfaces (surfaces). Several fine reviews on surface SHG spectroscopies have appeared recently. The present article will concentrate on some new applications of SHG: second harmonic microscopy (SHM) for microstructural characterization, SHG interferometry for the determination of absolute molecular orientation, surface analysis of microstructures, and some molecular interactions at the interfaces.

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“…Contrary to a scanning or serial technique, the stigmatic experiment provides the entire image in parallel. The image detector in our case is an intensified CCD camera with, for example, 2X2 Â 10 5 pixels [15]. If depth resolution is desired in a microscopy experiment the specimen must be scanned three-dimensionally with a tightly focused laser beam [16,17].…”

Section: Second-harmonic Imaging and Geometries For The Experimentsmentioning

confidence: 99%