In Situ In Vivo radiolabeling of polymer-coated hydroxyapatite nanoparticles to track their biodistribution in mice

Lobaz, Volodymyr; Pánek, Jiří; Vlk, Martin; Kozempel, Ján; Petřík, Miloš; Nový, Zbyněk; Gurská, Soňa; Znojek, Paweł; Štěpánek, Petr; Hrubý, Martin

doi:10.1016/j.colsurfb.2019.03.057

Cited by 15 publications

(14 citation statements)

References 35 publications

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“…We speculate that the main reason for the increase in HAP size is due to the agglomeration effect and not Pip loading or coating. After injection of the particles in vivo they are distributed in different organs depending on the different routes of in vivo application: sub-cutaneous, intramuscular, and intravenous injection [60,61]. Therefore, for future in vivo studies, the selection of intra-muscular, intradermal, or subcutaneous depot will be needed in order to avoid the barrier made by the reticulo-endothelial system (RES).…”

Section: Discussionmentioning

confidence: 99%

Effective Targeting of Colon Cancer Cells with Piperine Natural Anticancer Prodrug Using Functionalized Clusters of Hydroxyapatite Nanoparticles

et al. 2020

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Targeted drug delivery offers great opportunities for treating cancer. Here, we developed a novel anticancer targeted delivery system for piperine (Pip), an alkaloid prodrug derived from black pepper that exhibits anticancer effects. The tailored delivery system comprises aggregated hydroxyapatite nanoparticles (HAPs) functionalized with phosphonate groups (HAP-Ps). Pip was loaded into HAPs and HAP-Ps at pH 7.2 and 9.3 to obtain nanoformulations. The nanoformulations were characterized using several techniques and the release kinetics and anticancer effects investigated in vitro. The Pip loading capacity was >20%. Prolonged release was observed with kinetics dependent on pH, surface modification, and coating. The nanoformulations fully inhibited monolayer HCT116 colon cancer cells compared to Caco2 colon cancer and MCF7 breast cancer cells after 72 h, whereas free Pip had a weaker effect. The nanoformulations inhibited ~60% in HCT116 spheroids compared to free Pip. The Pip-loaded nanoparticles were also coated with gum Arabic and functionalized with folic acid as a targeting ligand. These functionalized nanoformulations had the lowest cytotoxicity towards normal WI-38 fibroblast cells. These preliminary findings suggest that the targeted delivery system comprising HAP aggregates loaded with Pip, coated with gum Arabic, and functionalized with folic acid are a potentially efficient agent against colon cancer.

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Section: Discussionmentioning

confidence: 99%

Effective Targeting of Colon Cancer Cells with Piperine Natural Anticancer Prodrug Using Functionalized Clusters of Hydroxyapatite Nanoparticles

et al. 2020

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“…According to these results, 177 Lu-DOTA-HA-PLGA-MTX NPs are a promising agent to treat rheumatic arthritis (Trujillo-Nolasco et al., 2019 ). Another study evaluated the biodistribution of hydroxyapatite (HA) PHPMA-TT NPs radiolabeled (Lobaz et al., 2019 ). They compared the biodistribution of 99m Tc-HEDP with 99m Tc-HEDP HAP NPs.…”

Section: Radioactive Polymeric Nanoparticles For Imaging and Therapymentioning

confidence: 99%

“…It was observed that 99m Tc-HEDP accumulated in the liver and bones, while 99m Tc-HEDP HAP NPs accumulated in the liver and spleen. Besides, HAP NPs labeled in vitro or in vivo with 99m Tc-HEDP had same distribution profile (Lobaz et al., 2019 ).…”

Section: Radioactive Polymeric Nanoparticles For Imaging and Therapymentioning

confidence: 99%

Radioactive polymeric nanoparticles for biomedical application

Wu¹,

Helal-Neto

Matos

et al. 2020

Drug Delivery

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Nowadays, emerging radiolabeled nanosystems are revolutionizing medicine in terms of diagnostics, treatment, and theranostics. These radionuclides include polymeric nanoparticles (NPs), liposomal carriers, dendrimers, magnetic iron oxide NPs, silica NPs, carbon nanotubes, and inorganic metal-based nanoformulations. Between these nano-platforms, polymeric NPs have gained attention in the biomedical field due to their excellent properties, such as their surface to mass ratio, quantum properties, biodegradability, low toxicity, and ability to absorb and carry other molecules. In addition, NPs are capable of carrying high payloads of radionuclides which can be used for diagnostic, treatment, and theranostics depending on the radioactive material linked. The radiolabeling process of nanoparticles can be performed by direct or indirect labeling process. In both cases, the most appropriate must be selected in order to keep the targeting properties as preserved as possible. In addition, radionuclide therapy has the advantage of delivering a highly concentrated absorbed dose to the targeted tissue while sparing the surrounding healthy tissues. Said another way, radioactive polymeric NPs represent a promising prospect in the treatment and diagnostics of cardiovascular diseases such as cardiac ischemia, infectious diseases such as tuberculosis, and other type of cancer cells or tumors.

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“…Hydroxyapatite (HAP) NPs are known to be biodegradable and biocompatible as HAP is the mineral component of bones. Geminal hydroxyalkylidene diphosphonates possess excellent affinity to HAP [ 23 ] and number of other inorganic nanoparticles [ 24 ] due to their structural similarity to diphosphate, which is present in the crystal lattice of HAP. Radiolabeled hydroxyalkylidene diphosphonates therefore accumulate in bones and are used as bone-seeking radiodiagnostics for bone metastases, fractures and sites of bone remodeling in general [ 25 ].…”

Section: Introductionmentioning

confidence: 99%

“…Recently, we published a comparison of antitumor efficacy of poly[ N -(2-hydroxypropyl)methacrylamide]-based and poly(2-ethyl-2-oxazoline)-based doxorubicin delivery systems in in vivo animal tumor models [ 26 ]. We also reported that HAP NPs can be coated with hydrophilic biocompatible polymers using terminal hydroxybisphosphonate groups and efficiently radiolabeled even in situ and in vivo in healthy mice with commercially available bone-seeking radiopharmaceuticals such as 99m Tc-hydroxyethylidene diphosphonate ( 99m Tc-HEDP) [ 23 ]. The main purpose of this study is to head-to-head compare biological behavior (blood circulation time, biodistribution including solid tumor and organ accumulation and biodegradation) of three leading polymers used as coating materials for biocompatibilization of NPs.…”

Section: Introductionmentioning

confidence: 99%

Head-To-Head Comparison of Biological Behavior of Biocompatible Polymers Poly(Ethylene Oxide), Poly(2-Ethyl-2-Oxazoline) and Poly[N-(2-Hydroxypropyl)Methacrylamide] as Coating Materials for Hydroxyapatite Nanoparticles in Animal Solid Tumor Model

et al. 2020

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Nanoparticles (NPs) represent an emerging platform for diagnosis and treatment of various diseases such as cancer, where they can take advantage of enhanced permeability and retention (EPR) effect for solid tumor accumulation. To improve their colloidal stability, prolong their blood circulation time and avoid premature entrapment into reticuloendothelial system, coating with hydrophilic biocompatible polymers is often essential. Most studies, however, employ just one type of coating polymer. The main purpose of this study is to head-to-head compare biological behavior of three leading polymers commonly used as “stealth” coating materials for biocompatibilization of NPs poly(ethylene oxide), poly(2-ethyl-2-oxazoline) and poly[N-(2-hydroxypropyl)methacrylamide] in an in vivo animal solid tumor model. We used radiolabeled biodegradable hydroxyapatite NPs as a model nanoparticle core within this study and we anchored the polymers to the NPs core by hydroxybisphosphonate end groups. The general suitability of polymers for coating of NPs intended for solid tumor accumulation is that poly(2-ethyl-2-oxazoline) and poly(ethylene oxide) gave comparably similar very good results, while poly[N-(2-hydroxypropyl)methacrylamide] was significantly worse. We did not observe a strong effect of molecular weight of the coating polymers on tumor and organ accumulation, blood circulation time, biodistribution and biodegradation of the NPs.

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In Situ In Vivo radiolabeling of polymer-coated hydroxyapatite nanoparticles to track their biodistribution in mice

Cited by 15 publications

References 35 publications

Effective Targeting of Colon Cancer Cells with Piperine Natural Anticancer Prodrug Using Functionalized Clusters of Hydroxyapatite Nanoparticles

Effective Targeting of Colon Cancer Cells with Piperine Natural Anticancer Prodrug Using Functionalized Clusters of Hydroxyapatite Nanoparticles

Radioactive polymeric nanoparticles for biomedical application

Head-To-Head Comparison of Biological Behavior of Biocompatible Polymers Poly(Ethylene Oxide), Poly(2-Ethyl-2-Oxazoline) and Poly[N-(2-Hydroxypropyl)Methacrylamide] as Coating Materials for Hydroxyapatite Nanoparticles in Animal Solid Tumor Model

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