In Situ Infrared Ellipsometry for Protein Adsorption Studies on Ultrathin Smart Polymer Brushes in Aqueous Environment

Kroning, Annika; Furchner, Andreas; Aulich, Dennis; Bittrich, Eva; Rauch, Sebastian; Uhlmann, Petra; Eichhorn, Klaus‐Jochen; Seeber, Michael; Luzinov, Igor; Kilbey, S. Michael; Lokitz, Bradley S.; Minko, Sergiy; Hinrichs, Karsten

doi:10.1021/am5075997

Cited by 44 publications

(29 citation statements)

References 59 publications

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“…There are a number of methods to investigate protein adsorption and its conformational changes, where atomic force microscopy (AFM), [29] time-of-flight secondary ion mass spectrometry, [30] Fourier transform infrared spectroscopy, [31] ellipsometry, [32] and quartz crystal microbalance [33] are the most adopted methods. Description of all these methods would go far beyond the scope of this review; therefore, we direct the reader to excellent reviews on instrumental methods for protein-surface interactions including the orientation and conformation of adsorbed proteins.…”

Section: Adsorption Influenced By Protein Molecular Propertiesmentioning

confidence: 99%

Controlling Protein Adsorption through Nanostructured Polymeric Surfaces

Firkowska‐Boden

Zhang

Jandt

2017

Adv Healthcare Materials

105

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The initial host response to healthcare materials' surfaces after implantation is the adsorption of proteins from blood and interstitial fluids. This adsorbed protein layer modulates the biological/cellular responses to healthcare materials. This stresses the significance of the surface protein assembly for the biocompatibility and functionality of biomaterials and necessitates a profound fundamental understanding of the capability to control protein–surface interactions. This review, therefore, addresses this by systematically analyzing and discussing strategies to control protein adsorption on polymeric healthcare materials through the introduction of specific surface nanostructures. Relevant proteins, healthcare materials' surface properties, clinical applications of polymer healthcare materials, fabrication methods for nanostructured polymer surfaces, amorphous, semicrystalline and block copolymers are considered with a special emphasis on the topographical control of protein adsorption. The review shows that nanostructured polymer surfaces are powerful tools to control the amount, orientation, and order of adsorbed protein layers. It also shows that the understanding of the biological responses to such ordered protein adsorption is still in its infancy, yet it has immense potential for future healthcare materials. The review, which is—as far as it is known—the first one discussing protein adsorption on nanostructured polymer surfaces, concludes with highlighting important current research questions.

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Section: Adsorption Influenced By Protein Molecular Propertiesmentioning

confidence: 99%

Controlling Protein Adsorption through Nanostructured Polymeric Surfaces

Firkowska‐Boden

Zhang

Jandt

2017

Adv Healthcare Materials

105

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“…Along with IRSE brush spectra, tan W brush , reference spectra of a clean silicon wedge, tan W Si , were always measured under the same experimental conditions. Ex situ IRSE brush measurements were performed at the polymer-air interface under an incidence angle of 65 after drying the brushes in water-vapor-free atmosphere for at least 1 h. In situ ellipsometric measurements in H 2 O and D 2 O were carried out with a specifically designed flow cell [28][29][30] in backside illumination of the infrared-transparent silicon substrates under an incidence angle of 52. 5 at the air-silicon interface, resulting in a %13…”

Section: B Brush Preparationmentioning

confidence: 99%

Probing carbonyl–water hydrogen-bond interactions in thin polyoxazoline brushes

et al. 2016

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Temperature-responsive oxazoline-based polymer brushes have gained increased attention as biocompatible surfaces. In aqueous environment, they can be tuned between hydrophilic and hydrophobic behavior triggered by a temperature stimulus. This transition is connected with changes in molecule–solvent interactions and results in a switching of the brushes between swollen and collapsed states. This work studies the temperature-dependent interactions between poly(2-oxazoline) brushes and water. In detail, thermoresponsive poly(2-cyclopropyl-2-oxazoline), nonresponsive hydrophilic poly(2-methyl-2-oxazoline), as well as a copolymer of the two were investigated with in situ infrared ellipsometry. Focus was put on interactions of the brushes' carbonyl groups with water molecules. Different polymer–water interactions could be observed and assigned to hydrogen bonding between C=O groups and water molecules. The switching behavior of the brushes in the range of 20–45 °C was identified by frequency shifts and intensity changes of the amide I band.

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“…As a special class of macromolecules, smart polymers manifest impressive response to physiochemical change if their circumstance has slight interference, for example, changes on PH value, ionic disturbance, magnetic field, light, and temperature (see Hai and Broekmann [25], do Nascimento Marques et al [26], and Kroning et al [27]). Thus, smart polymers are also denoted by environmentally responsive systems or stimuli responsive ones.…”

Section: Motivationmentioning

confidence: 99%

Topological Indices Study of Molecular Structure in Anticancer Drugs

Gao

Wang²,

Farahani³

2016

Journal of Chemistry

139

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Numerous studies indicate that there is strong inherent relationship between the chemical characteristics of chemical compounds and drugs (e.g., boiling point and melting point) and their molecular structures. Topological indices defined on these chemical molecular structures can help researchers better understand the physical features, chemical reactivity, and biological activity. Thus, the study of the topological indices on chemical structure of chemical materials and drugs can make up for lack of chemical experiments and can provide a theoretical basis for the manufacturing of drugs and chemical materials. In this paper, we focus on the family of smart polymer which is widely used in anticancer drugs manufacturing. Several topological indices are determined in view of edge dividing methods, and these results remedy the lack of chemical and medicine experiments thus providing the theoretical basis for pharmaceutical engineering.

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In Situ Infrared Ellipsometry for Protein Adsorption Studies on Ultrathin Smart Polymer Brushes in Aqueous Environment

Cited by 44 publications

References 59 publications

Controlling Protein Adsorption through Nanostructured Polymeric Surfaces

Controlling Protein Adsorption through Nanostructured Polymeric Surfaces

Probing carbonyl–water hydrogen-bond interactions in thin polyoxazoline brushes

Topological Indices Study of Molecular Structure in Anticancer Drugs

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