In-situ ionic crosslinking of 3D bioprinted cell-hydrogel constructs for mechanical reinforcement and improved cell growth

Lee, Ji Eun; Heo, Seung Won; Kim, Chae Hwa; Park, Seong Je; Park, Suk-Hee; Kim, Tae Hee

doi:10.1016/j.bioadv.2023.213322

Cited by 10 publications

(1 citation statement)

References 54 publications

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“…Hydrogels can be produced by physical gelation methods such as thermo-gelation [15], ionic crosslinking [16], pH sensitivity [17], and host-guest interaction [18] or by chemical crosslinking methods such as photopolymerization [19], click chemistry [20], enzymemediated crosslinking [21], and through the use of a crosslinking agent [22] establishing covalent bonds between polymeric chains to form an intricated network [23][24][25].…”

Section: Of 23mentioning

confidence: 99%

Building Fucoidan/Agarose-Based Hydrogels as a Platform for the Development of Therapeutic Approaches against Diabetes

et al. 2023

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Current management for diabetes has stimulated the development of versatile 3D-based hydrogels as in vitro platforms for insulin release and as support for the encapsulation of pancreatic cells and islets of Langerhans. This work aimed to create agarose/fucoidan hydrogels to encapsulate pancreatic cells as a potential biomaterial for diabetes therapeutics. The hydrogels were produced by combining fucoidan (Fu) and agarose (Aga), marine polysaccharides derived from the cell wall of brown and red seaweeds, respectively, and a thermal gelation process. The agarose/fucoidan (AgaFu) blended hydrogels were obtained by dissolving Aga in 3 or 5 wt % Fu aqueous solutions to obtain different proportions (4:10; 5:10, and 7:10 wt). The rheological tests on hydrogels revealed a non-Newtonian and viscoelastic behavior, while the characterization confirmed the presence of the two polymers in the structure of the hydrogels. In addition, the mechanical behavior showed that increasing Aga concentrations resulted in hydrogels with higher Young’s modulus. Further, the ability of the developed materials to sustain the viability of human pancreatic cells was assessed by encapsulation of the 1.1B4HP cell line for up to 7 days. The biological assessment of the hydrogels revealed that cultured pancreatic beta cells tended to self-organize and form pseudo-islets during the period studied.

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Section: Of 23mentioning

confidence: 99%

Building Fucoidan/Agarose-Based Hydrogels as a Platform for the Development of Therapeutic Approaches against Diabetes

et al. 2023

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Nanostructured Biopolymer‐Based Constructs for Cartilage Regeneration: Fabrication Techniques and Perspectives

Sharma,

Satapathy

2024

Macromolecular Bioscience

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The essential functions of cartilage, such as shock absorption and resilience, are hindered by its limited regenerative capacity. Although current therapies alleviate symptoms, novel strategies for cartilage regeneration are desperately needed. Recent developments in 3D constructs aim to address this challenge by mimicking the intrinsic characteristics of native cartilage using biocompatible materials, with a significant emphasis on both functionality and stability. Through fabrication methods like 3D printing and electrospinning, researchers are making progress in cartilage regeneration; nevertheless, it is still very difficult to translate these advances into clinical practice. The review emphasizes the importance of integrating various fabrication techniques to create stable 3D constructs. Meticulous design and material selection are required to achieve seamless cartilage integration and durability. The review outlines the need to address these challenges and focuses on the latest developments in the production of hybrid 3D constructs based on biodegradable and biocompatible polymers. Furthermore, it acknowledges the limitations of current research and provides perspectives on potential avenues for effectively regenerating cartilage defects in the future.This article is protected by copyright. All rights reserved

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Bioactive Ions-Loaded Bioinks Primed for 3D Printing of Artificial Tissues

Jo,

Kang,

Kim

et al. 2024

Biomedical Materials & Devices

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In-situ ionic crosslinking of 3D bioprinted cell-hydrogel constructs for mechanical reinforcement and improved cell growth

Cited by 10 publications

References 54 publications

Building Fucoidan/Agarose-Based Hydrogels as a Platform for the Development of Therapeutic Approaches against Diabetes

Building Fucoidan/Agarose-Based Hydrogels as a Platform for the Development of Therapeutic Approaches against Diabetes

Nanostructured Biopolymer‐Based Constructs for Cartilage Regeneration: Fabrication Techniques and Perspectives

Bioactive Ions-Loaded Bioinks Primed for 3D Printing of Artificial Tissues

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