In-Situ IR Spectral Measurement in Organic Matrix-Mediated Hydroxyapatite Formation

Abstract

L@}gbNXU±ÉaeénChLVAp^Cg`¬ßöɨ¯éÔOªõ»Ìêªè ²¡ö×EFJF¢Þ E ¶îrVÞ Þ EnLiEcOÞ Þ YAEZp¤C4638560 ¤m §¼Ã®sçRaeºui¡´226698 Þ ÈwZpU»@\ CRESTC3320012 éÊ §ìûs{¬ 418 Þ Þ¿EÞ¿¤@\C3050044 ïé §ÂÎsÀØ 11Organic templatedirected crystallization of hydroxyapatite (HAp) was studied insitu via Fourier transform IR external reection spectroscopy. Langmuir monolayer of arachidic acid was formed on a surface of simulated body uid, which possesses inorganic ion concentrations and a pH value almost equal to those of human blood plasma. Owing to the chemical interactions between inorganic ions and carboxyl groups, heterogeneous nuclea tion of HAp occurred below the monolayer. The absorbances of the antisymmetric and symmetric methylene stretching bands in the IR spectra varied during the nucleation process, which means that conformational change of hydrocarbon chain occurred. Interfacial interactions between organic templates and induced crystals are often discussed only through static experimental models. However, the organic matrices should also be treated as elas tic template and their structural changes due to the formation of HAp crystals should be recognized, when the biomineralization mechanism is discussed.

In vitro mineralisation of grafted ePTFE membranes carrying carboxylate groups

“…Previous studies have found similar spectral features of carbonated HAP after SBF immersion of ePTFE samples grafted with MOEP in methanol [8] , in the aqueous phase of a two-phase system [9] as well as on a branched PMOEP film [34] . This finding also correlates with the FTIR spectrum obtained for the mineral formed in SBF on an arachidic acid Langmuir monolayer [35] and the precipitate formed in SBF when pH is kept constant during precipitation [36] . The intensity of the carbonate bands relative to the phosphate band is much higher for samples AA(0.3)-R and AA(1.3)-R and the carbonate band at 875 cm −1 is less pronounced in these samples compared to the two high graft extent samples.…”

Nucleation and growth of CuSO4·5H2O crystals on the liquid state stearic acid Langmuir–Blodgett film

“…It is very clear that the performances of crystals such as polymorphism and orientation strongly depend on the structure and property of film templates. On the other hand, Sato and Charych studied the structural changes of Langmuir film at air-water interface and they found that the structures of the organic films vary (dynamic molecular rearrangements) during the nucleation process [15,16]. However, structural change in an LB film template tuned by temperature has received little attention in the crystal growth field.…”