In Situ, Label‐Free DNA Detection Using Organic Transistor Sensors

Khan, Hadayat Ullah; Roberts, Mark; Johnson, Olasupo B.; Förch, Renate; Knoll, Wolfgang; Bao, Zhenan

doi:10.1002/adma.201000790

Cited by 174 publications

(185 citation statements)

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“…Organic thin film transistors (OTFTs) have been used as transducers for chemical, biological and pressure-sensing devices [19][20][21][22][23][24][25][26] . Recently, we reported microstructured polydimethylsiloxane (PDMS) as dielectric layer for flexible capacitive pressure sensors with 0.55 kPa À 1 sensitivity and response time in the milliseconds range 3 .…”

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confidence: 99%

Flexible polymer transistors with high pressure sensitivity for application in electronic skin and health monitoring

et al. 2013

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Flexible pressure sensors are essential parts of an electronic skin to allow future biomedical prostheses and robots to naturally interact with humans and the environment. Mobile biomonitoring in long-term medical diagnostics is another attractive application for these sensors. Here we report the fabrication of flexible pressure-sensitive organic thin film transistors with a maximum sensitivity of 8.4 kPa À 1 , a fast response time of o10 ms, high stability over 415,000 cycles and a low power consumption of o1 mW. The combination of a microstructured polydimethylsiloxane dielectric and the high-mobility semiconducting polyisoindigobithiophene-siloxane in a monolithic transistor design enabled us to operate the devices in the subthreshold regime, where the capacitance change upon compression of the dielectric is strongly amplified. We demonstrate that our sensors can be used for non-invasive, high fidelity, continuous radial artery pulse wave monitoring, which may lead to the use of flexible pressure sensors in mobile health monitoring and remote diagnostics in cardiovascular medicine.

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Flexible polymer transistors with high pressure sensitivity for application in electronic skin and health monitoring

et al. 2013

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“…OFET sensors can also work in water (19,20) as well as in the subvolt bias regime (20,21). Specificity relies on device structures that either involve enzymatic reactions [with FET electrochemical transduction (22)] or two-layer architectures, including a bioactive film deposited on top of the channel organic semiconductor (18,23,24). The reference electrode issue remains in electrochemical OFETs, while in the two-layer architecture the recognition event takes place far from the semiconductor/dielectric interface affecting the 2D electronic transport only indirectly (10).…”

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confidence: 99%

Interfacial electronic effects in functional biolayers integrated into organic field-effect transistors

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et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

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Biosystems integration into an organic field-effect transistor (OFET) structure is achieved by spin coating phospholipid or protein layers between the gate dielectric and the organic semiconductor. An architecture directly interfacing supported biological layers to the OFET channel is proposed and, strikingly, both the electronic properties and the biointerlayer functionality are fully retained. The platform bench tests involved OFETs integrating phospholipids and bacteriorhodopsin exposed to 1-5% anesthetic doses that reveal drug-induced changes in the lipid membrane. This result challenges the current anesthetic action model relying on the so far provided evidence that doses much higher than clinically relevant ones (2.4%) do not alter lipid bilayers' structure significantly. Furthermore, a streptavidin embedding OFET shows label-free biotin electronic detection at 10 parts-per-trillion concentration level, reaching state-of-the-art fluorescent assay performances. These examples show how the proposed bioelectronic platform, besides resulting in extremely performing biosensors, can open insights into biologically relevant phenomena involving membrane weak interfacial modifications.organic electronics | analytical bioassay | electronic biodetection

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“…Upon the injection of a 1 µM buffer solution of the target strand the increase in I DS measured during 6 min nearly reached the saturation value and could be fitted by a single exponential increase, cf. the red curve in Figure 8, from which the electronic k on rate constant could be determined [5].…”

Section: Electronic Recording Of Surface Hybridization Reactionsmentioning

confidence: 99%

“…In order to make sure that the results obtained by optical detection-in our case by surface-plasmon field enhanced fluorescence spectroscopy (SPFS) of interfacial binding reactions between surfaceattached oligonucleotide capture probe strands and complementary target strands from solution-can be directly compared to the data derived from an electronic transducer device-the ones presented and discussed here from an organic thin film transistor (OTFT)-we designed an interfacial architecture that could be used for both transducer platforms because it took into account the technical needs of both experiments [5].…”

Section: Anmentioning

confidence: 99%