In situ localization and changes in the expression level of transcripts related to intercellular transport and phloem loading in leaves of maize (Zea mays L.) treated with low temperature

Bilska-Kos, Anna; Grzybowski, Marcin; Jończyk, Maciej; Sowiński, Paweł

doi:10.1007/s11738-016-2151-5

Cited by 14 publications

(13 citation statements)

References 44 publications

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“…After cutting, the loss of pressure is thought to cause the accumulation of parietal proteins and potentially ER against the sieve plate (van Bel, 2003). Immuno‐gold labeling of aquaporins using electron microscopy has shown the presence of aquaporins near ER filaments (Bilska‐Kos et al, 2016), providing support to our observations of high aquaporin signal intensity within these so‐called “slime bodies”. However, this still begs the question of why there are so many PIP1s within internal compartments of sieve elements in the first place?…”

Section: Discussionsupporting

confidence: 78%

Are phloem sieve tubes leaky conduits supported by numerous aquaporins?

Stanfield

Hacke

Laur

2017

American J of Botany

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PREMISE OF THE STUDY: Aquaporin membrane water channels have been previously identified in the phloem of angiosperms, but currently their cellular characterization is lacking, especially in tree species. Pinpointing the cellular location will help generate new hypotheses of how membrane water exchange facilitates sugar transport in plants. METHODS: We studied histological sections of balsam poplar (Populus balsamifera L.) in leaf, petiole, and stem organs. Immuno‐labeling techniques were used to characterize the distribution of PIP1 and PIP2 subfamilies of aquaporins along the phloem pathway. Confocal and super resolution microscopy (3D‐SIM) was used to identify the localization of aquaporins at the cellular level. KEY RESULTS: Sieve tubes of the leaf lamina, petiole, and stem were labeled with antibodies directed at PIP1s and PIP2s. While PIP2s were mostly observed in the plasma membrane, PIP1s showed both an internal membrane and plasma membrane labeling pattern. CONCLUSIONS: The specificity and consistency of PIP2 labeling in sieve element plasma membranes points to high water exchange rates between sieve tubes and adjacent cells. The PIP1s may relocate between internal membranes and the plasma membrane to facilitate dynamic changes in membrane permeability of sieve elements in response to changing internal or environmental conditions. Aquaporin‐mediated changes in membrane permeability of sieve tubes would also allow for some control of radial exchange of water between xylem and phloem.

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Section: Discussionsupporting

confidence: 78%

Are phloem sieve tubes leaky conduits supported by numerous aquaporins?

Stanfield

Hacke

Laur

2017

American J of Botany

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“…6,7,8). Thus, it may be supposed that the changes in the structure of chloroplasts leading to disorders in the photosynthetic apparatus can affect photosynthesis and the transport of photosynthetic products, as has been previously observed for other chilling-sensitive maize line (Bilska and Sowiński 2010;Bilska 2013;Bilska-Kos et al 2016a). The effect of cold on the photosynthetic apparatus was also visible in the 5th maize leaf-the starch grains were noted in bundle sheath chloroplasts in the leaves of the chilling-sensitive line (Sowiński et al 2005), what has been confirmed by gas-liquid chromatography in another work (Sowiński et al 1999).…”

Section: Discussionmentioning

confidence: 80%

“…SPS catalyzes the conversion of fructose-6-phosphate and uridine diphosphate-glucose (UDP-glucose) into sucrose-6-phosphate which is a substrate in the synthesis of sucrose (Winter and Huber 2000 ; Chen et al 2005 ). SUS catalyzes the conversion of sucrose and nucleoside diphosphate into the corresponding nucleoside diphosphate-glucose and fructose, and provides substrates for cellulose and starch biosynthesis: UDP-glucose and adenosine diphosphate-glucose (ADP-glucose) (Amor et al 1995 ; Haigler et al 2001 ; Baroja-Fernández et al 2009 ).…”

Section: Introductionmentioning

confidence: 99%

Sucrose phosphate synthase (SPS), sucrose synthase (SUS) and their products in the leaves of Miscanthus × giganteus and Zea mays at low temperature

Bilska-Kos

Mytych

Suski

et al. 2020

Planta

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“…NaCl stress protein1 (GRMZM2G015605, up-regulated in maize) has an A. thaliana ortholog AT3G05880.1 described in the TAIR database as induced by low temperature, dehydration, salt stress, and ABA ( ). Remorin (GRMZM2G099239, up-regulated) has a maize paralog GRMZM2G081949 which was down-regulated after prolonged cold-treatment both at moderate and severe cold ( Bilska-Kos et al, 2016 ), i.e., it responded in the opposite direction to GRMZM2G099239. Notably, it showed up-regulation in a cold-tolerant inbred line after short cold-treatment (ibid.).…”

Section: Discussionmentioning

confidence: 99%

Maize Response to Low Temperatures at the Gene Expression Level: A Critical Survey of Transcriptomic Studies

et al. 2020

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Maize is a cold-sensitive plant whose physiological reactions to sub-optimal temperatures are well understood, but their molecular foundations are only beginning to be deciphered. In an attempt to identify key genes involved in these reactions, we surveyed several independent transcriptomic studies addressing the response of juvenile maize to moderate or severe cold. Among the tens of thousands of genes found to change expression upon cold treatment less than 500 were reported in more than one study, indicating an astonishing variability of the expression changes, likely depending on the experimental design and plant material used. Nearly all these “common” genes were specific to either moderate or to severe cold and formed distinct interaction networks, indicating fundamentally different responses. Moreover, down-regulation of gene expression dominated strongly in moderate cold and up-regulation prevailed in severe cold. Very few of these genes have ever been mentioned in the literature as cold-stress–related, indicating that most response pathways remain poorly known at the molecular level. We posit that the genes identified by the present analysis are attractive candidates for further functional studies and their arrangement in complex interaction networks indicates that a re-interpretation of the present state of knowledge on the maize cold-response is justified.

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In situ localization and changes in the expression level of transcripts related to intercellular transport and phloem loading in leaves of maize (Zea mays L.) treated with low temperature

Cited by 14 publications

References 44 publications

Are phloem sieve tubes leaky conduits supported by numerous aquaporins?

Are phloem sieve tubes leaky conduits supported by numerous aquaporins?

Sucrose phosphate synthase (SPS), sucrose synthase (SUS) and their products in the leaves of Miscanthus × giganteus and Zea mays at low temperature

Maize Response to Low Temperatures at the Gene Expression Level: A Critical Survey of Transcriptomic Studies

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