In situ localization of nitric oxide synthase and direct evidence of NO production in rat retinal ganglion cells

Tsumamoto, Yuichi; Yamashita, Keisuke; Takumida, Masaya; Okada, Koji; Mukai, Satoshi; Shinya, Makoto; Yamashita, Hidetoshi; Mishima, Hiromu K.

doi:10.1016/s0006-8993(02)02289-8

Cited by 24 publications

(13 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Using a recently developed fluorometric method that enables the direct detection of intracellular NO within individual cells (Kojima et al 1998;Brown et al 1999;Blute et al 2000;Tsumamoto et al 2002;Blute et al 2003;Li et al 2003b;Rathel et al 2003), in combination with immunohistochemistry and electrophysiology, we obtained novel information on the cellular sources, targets and functional relevance of tonic NO production within the MNS. To our knowledge, this is the first report to directly visualize intracellular NO availability in MNCs.…”

Section: Discussionmentioning

confidence: 99%

Cellular sources, targets and actions of constitutive nitric oxide in the magnocellular neurosecretory system of the rat

Stern

Zhang

2005

The Journal of Physiology

View full text Add to dashboard Cite

Nitric oxide (NO) is a key activity-dependent modulator of the magnocellular neurosecretory system (MNS) during conditions of high hormonal demand. In addition, recent studies support the presence of a functional consitutive NO tone. The aim of this study was to identify the cellular sources, targets, signalling mechanisms and functional relevance of constitutive NO production within the supraoptic nucleus (SON). Direct visualization of intracellular NO, along with neuronal nitric oxide synthase (nNOS) and cGMP immunohistochemisty, was used to study the cellular sources and targets of NO within the SON, respectively. Our results support the presence of a strong NO basal tone within the SON, and indicate that vasopressin (VP) neurones constitute the major neuronal source and target of basal NO. NO induced-fluorescence and cGMP immunoreactivity (cGMPir) were also found in the glia and microvasculature of the SON, suggesting that they contribute as sources/targets of NO within the SON. cGMPir was also found in association with glutamic acid decarboxylase 67 (GAD67)-and vesicular glutamate transporter 2 (VGLUT2)-positive terminals. Glutamate, acting on NMDA and possibly AMPA receptors, was found to be an important neurotransmitter driving basal NO production within the SON. Finally, electrophysiological recordings obtained from SON neurones in a slice preparation indicated that constitutive NO efficiently restrains ongoing firing activity of these neurones. Furthermore, phasically active (putative VP) and continuously firing neurones appeared to be influenced by NO originating from different sources. The potential roles for basal NO as an autocrine signalling molecule, and one that bridges neuronal-glial-vascular interactions within the MNS are discussed.

show abstract

Section: Discussionmentioning

confidence: 99%

Cellular sources, targets and actions of constitutive nitric oxide in the magnocellular neurosecretory system of the rat

Stern

Zhang

2005

The Journal of Physiology

View full text Add to dashboard Cite

show abstract

“…eNOS did not appear to play a major role in NO production because the NO production was inhibited by 7-NI, while immunohistochemically, both nNOS and eNOS were expressed in retinal ganglion neurons [75]. It is suggested that the synthesis of NO by eNOS may represent a compensatory mechanism in the absence of nNOS.…”

Section: Role Of No On Atp-induced Ca 2+ Signalling In Ihcs and Ohcsmentioning

confidence: 91%

“…It has been shown that NO production induced by glutamate in cultured retinal ganglion neurons was considered to be mainly associated with nNOS [75]. eNOS did not appear to play a major role in NO production because the NO production was inhibited by 7-NI, while immunohistochemically, both nNOS and eNOS were expressed in retinal ganglion neurons [75].…”

Section: Role Of No On Atp-induced Ca 2+ Signalling In Ihcs and Ohcsmentioning

confidence: 98%

Role of nitric oxide on purinergic signalling in the cochlea

Harada

2010

Purinergic Signalling

View full text Add to dashboard Cite

In the inner ear, there is considerable evidence that extracellular adenosine 5′-triphosphate (ATP) plays an important role in auditory neurotransmission as a neurotransmitter or a neuromodulator, although the potential role of adenosine signalling in the modulation of auditory neurotransmission has also been reported. The activation of ligand-gated ionotropic P2X receptors and G proteincoupled metabotropic P2Y receptors has been reported to induce an increase of intracellular Ca 2+ concentration ([Ca 2+ ] i ) in inner hair cells (IHCs), outer hair cells (OHCs), spiral ganglion neurons (SGNs), and supporting cells in the cochlea. ATP may participate in auditory neurotransmission by modulating [Ca 2+ ] i in the cochlear cells. Recent studies showed that extracellular ATP induced nitric oxide (NO) production in IHCs, OHCs, and SGNs, which affects the ATP-induced Ca 2+ response via the NO-cGMP-PKG pathway in those cells by a feedback mechanism. A cross-talk between NO and ATP may therefore exist in the auditory signal transduction. In the present article, I review the role of NO on the ATP-induced Ca 2+ signalling in IHCs and OHCs. I also consider the possible role of NO in the ATP-induced Ca 2+ signalling in SGNs and supporting cells.

show abstract

“…Two recent reports addressing the localization of neuronal NOS in neonatal rat retina (13,14) have shown that cell bodies of RGCs also exhibit immunoreactivity for this NOS isoform. Conceivably, NO generated from either amacrine cells or RGCs exerts profound influences on the survival of RGCs.…”

mentioning

confidence: 99%

Neuronal Nitric Oxide Synthase Is Crucial for Ganglion Cell Death in Rat Retinal Explant Cultures

et al. 2004

View full text Add to dashboard Cite

Abstract. We examined possible involvement of nitric oxide synthase (NOS) on ganglion cell death in explant cultures of neonatal rat retina. Survival of retinal ganglion cells was significantly prolonged by a broad-spectrum NOS inhibitor N w -nitro-L-arginine methylester. NADPH diaphorase staining revealed a diffused distribution of NOS activity in neuropils of the inner plexiform layer as well as several neurons in the inner nuclear layer. Moreover, 7-nitroindazole but not aminoguanidine promoted the survival of retinal ganglion cells. These results suggest a crucial role of neuronal NOS-derived nitric oxide in retinal ganglion cell death.

show abstract

In situ localization of nitric oxide synthase and direct evidence of NO production in rat retinal ganglion cells

Cited by 24 publications

References 53 publications

Cellular sources, targets and actions of constitutive nitric oxide in the magnocellular neurosecretory system of the rat

Cellular sources, targets and actions of constitutive nitric oxide in the magnocellular neurosecretory system of the rat

Role of nitric oxide on purinergic signalling in the cochlea

Neuronal Nitric Oxide Synthase Is Crucial for Ganglion Cell Death in Rat Retinal Explant Cultures

Contact Info

Product

Resources

About