In situ localization of two haemoglobin gene clusters in the chromosomes of 13 species of Chironomus

Schmidt, Erwin R.; Keyl, H. G.; Hankeln, T

doi:10.1007/bf00330701

Cited by 43 publications

(30 citation statements)

References 15 publications

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“…The nonradioactive digoxigenin-labeled DNA probe was prepared according to the method of random-primed labeling (24), and hybridization and detection of the hybridized fragment were carried out by the procedures of Langer-Safer et al (25) and Schmidt et al (26).…”

Section: Methodsmentioning

confidence: 99%

Selenium Metabolism in Drosophila

Zhou

Park

Moustafa

et al. 1999

Journal of Biological Chemistry

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The selenocysteine (Sec) tRNA population in Drosophila melanogaster is aminoacylated with serine, forms selenocysteyl-tRNA, and decodes UGA. The K m of Sec tRNA and serine tRNA for seryl-tRNA synthetase is 6.67 and 9.45 nM, respectively. Two major bands of Sec tRNA were resolved by gel electrophoresis. Both tRNAs were sequenced, and their primary structures were indistinguishable and colinear with that of the corresponding single copy gene. They are 90 nucleotides in length and contain three modified nucleosides, 5-methylcarboxymethyluridine, N 6 -isopentenyladenosine, and pseudouridine, at positions 34, 37, and 55, respectively. Neither form contains 1-methyladenosine at position 58 or 5-methylcarboxymethyl-2-O-methyluridine, which are characteristically found in Sec tRNA of higher animals. We conclude that the primary structures of the two bands of Sec tRNA resolved by electrophoresis are indistinguishable by the techniques employed and that Sec tRNAs in Drosophila may exist in different conformational forms. The Sec tRNA gene maps to a single locus on chromosome 2 at position 47E or F. To our knowledge, Drosophila is the lowest eukaryote in which the Sec tRNA population has been characterized to date.

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Section: Methodsmentioning

confidence: 99%

Selenium Metabolism in Drosophila

Zhou

Park

Moustafa

et al. 1999

Journal of Biological Chemistry

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“…thummi/C, th. piger subspecies hybrid and procedures for in situ hybridization were as described by Schmidt et al (1988).…”

Section: Methodsmentioning

confidence: 99%

“…chromosomes Schmidt et al 1988), we expected that the ctt-1 and ctt-lA genes would either reside at the ctt-4 locus or possibly at a unique chromosomal band. Surprisingly, DNA of genomic clone MW hybridized in situ to chromosome arm D of a hybrid of C. th.…”

Section: In Situ Hybridization To Polytene Chromosomesmentioning

confidence: 99%

“…The localization of a monomeric Gb gene cluster (containing genes encoding CTT-III and CTT-IV) and a homodimeric Gb gene cluster (containing genes encoding CTT-VIIB variants) by in situ hybridization to the chironomid polytene salivary gland chromosomes was reported earlier Schmidt et al 1988). The CTT-III/IV cluster was localized to position Alb2 on arm E of chromosome III, while the CTT-VIIB probe hybridized to position F2b3 of arm D on chromosome II in C. th.…”

Section: Introductionmentioning

confidence: 99%

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Sequence and evolution of the gene for the monomeric globin I and its linkage to genes coding for dimeric globins in the insect Chironomus thummi

et al. 1995

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We isolated genomic clones containing sequences encoding globins I and IA from a Chironomus thummi thummi genomic library. Three clones contain globin IA (ctt-1A) genes, while one contains a globin I (ctt-1) gene. The coding regions of the four genes are identical except for the single base substitution accounting for the globin I/IA polymorphism. The noncoding DNA flanking the coding region is more than 98% similar, confirming a previous hypothesis that the globin ctt-1 and ctt-1A genes are alleles. Hemoglobins I and IA are monomeric in the insect hemolymph. Earlier in situ hybridization studies suggested that monomeric and dimeric globin genes are clustered at different chromosomal loci. In situ hybridization of ctt-1 DNA to polytene salivary gland chromosomes places the ctt-1 gene on the same band as genes for the dimeric globins II beta and VIIB, forcing revision of the earlier hypothesis that genes for monomeric and dimeric globin genes are at different loci. The evolution of the ctt-1 and ctt-1A alleles and of the two globin gene loci are discussed.

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“…The present work is intended to develop a reliable protocol, comparable with other published methods for in situ hybridization [7][8][9], in order to implement FISH in our further projects concerning mapping genes and chromosomal aberrations in plant, animal and in human chromosomes. Subsequent improving of the technique will allow us to obtain a more refined location of interesting target sequences.…”

mentioning

confidence: 99%

Localizing genes in Drosophila melanogaster polytene chromosomes by fluorescence in situ hybridization

Gavrilă¹,

Ecovoiu²,

Georgescu³

2001

J Cellular Molecular Medi

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This paper describes a method for the identification of single copy genes in Drosophila melanogaster polytene chromosomes, using fluorescence in situ hybridization (FISH). We demonstrate the detection of white (w), a gene previously mapped to 1‐1.5 region of the linkage map, and to 3C2 region of the cytogenetic map of X chromosome. Squash preparations of polytene chromosomes from salivary glands dissected out from third instar larvae of Drosophila melanogaster were denatured and subjected to hybridization with a digoxigenin labeled probe, corresponding to mini‐white gene. The preparations were then washed and incubated with antidigoxigenin‐fluorescein antibodies. After removal of the nonspecifically bound antibodies, the polytene chromosomes were counterstained with propidium iodide. Fluorescence microscopy revealed white locus in the X chromosome in a subterminal location, in agreement with the above mentioned maps. The protocol is efficient and adaptable for simultaneously multiple signal detection.

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In situ localization of two haemoglobin gene clusters in the chromosomes of 13 species of Chironomus

Cited by 43 publications

References 15 publications

Selenium Metabolism in Drosophila

Selenium Metabolism in Drosophila

Sequence and evolution of the gene for the monomeric globin I and its linkage to genes coding for dimeric globins in the insect Chironomus thummi

Localizing genes in Drosophila melanogaster polytene chromosomes by fluorescence in situ hybridization

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