In situ magnetoelectric generation of nitric oxide and electric stimulus for nerve therapy by wireless chargeable molybdenum carbide octahedrons

Chan, Yi-Chieh; Lin, Yow-Jian; Liu, Hsiu‐Ching; Hsu, Ru‐Siou; Chiang, Min‐Ren; Wang, Liwen; Chou, Tsu‐Chin; Lu, Tsai‐Te; Lee, I‐Chi; Chu, Li‐An; Hu, Shang‐Hsiu

doi:10.1016/j.nantod.2023.101935

Cited by 18 publications

(5 citation statements)

References 47 publications

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“…For the AMF-treated group, a magnetic field at a power of 3.2 kW and a frequency of 1 MHz was applied for 5 min per day until the mice were sacrificed. Additional experimental details are as described in Chan et al, 2023 Mice were sacrificed 45 d after TBI induction.…”

Section: Methodsmentioning

confidence: 99%

Revealing intact neuronal circuitry in centimeter-sized formalin-fixed paraffin-embedded brain

Lin,

Wang,

Chen

et al. 2024

eLife

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Tissue clearing and labeling techniques have revolutionized brain-wide imaging and analysis, yet their application to clinical formalin-fixed paraffin-embedded (FFPE) blocks remains challenging. We introduce MOCAT, a novel method for efficiently clearing and labeling centimeter-thick FFPE specimens using elevated temperature and concentrated detergents. MOCAT with multi-round immunolabeling reveals neuron circuitry regulating multiple neurotransmitter systems in a whole FFPE mouse brain, and is able to be used as the evaluation of disease treatment efficiency. MOCAT also supports expansion microscopy and can be performed on a non-sectioned 15-year-old FFPE specimen. Thus, MOCAT represents a feasible approach for researching archived FFPE specimens for future neuroscientific and 3D neuropathological analyses.

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Section: Methodsmentioning

confidence: 99%

Revealing intact neuronal circuitry in centimeter-sized formalin-fixed paraffin-embedded brain

Lin,

Wang,

Chen

et al. 2024

eLife

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“…Additional experimental details are as described in Chan et al. [68] Mice were sacrificed 45 days after TBI induction.…”

Section: Methodsmentioning

confidence: 99%

Revealing intact neuronal circuitry in centimeter-sized formalin-fixed paraffin-embedded brain

Lin

Wang

Chen

et al. 2022

Preprint

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Current tissue clearing and labeling approaches require freshly prepared samples to avoid problems related to over-fixation, such as loss of antigenicity or difficulty of tissue clearing. Thus, the predicament of specimens with long-term preservation limits the application of state-of-art technology to archived human brain tissues. Here, we present the development and validation of multiplex labeling of centimeter-sized archived tissue (MOCAT), a formalin-fixed paraffin-embedding (FFPE) based sample preservation and delipidation technique for centimeter-level tissue clearing and immunolabeling. We demonstrate that MOCAT sufficiently delipidates whole mouse brain and human brain blocks and is superior to 10% formalin for long-term antigenicity preservation; moreover, the spatial information of biomarkers offered by MOCAT-processed intact mouse brains is identical to that offered by freshly prepared SDS-delipidated intact mouse brains. MOCAT not only satisfies the need for long-term tissue storage before performing volumetric multiplex imaging for research purposes but also has the potential to be used in human clinical examination.

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“…Therefore, a 3D-printed implantable microneedle composed of a magnetoelectric MoCx–Cu MOFs and a NO donor, S -nitrosoglutathione (GSNO), was developed by Chan et al for the magnetoelectric responsive repair of traumatic brain injury. 58 Under the action of alternating magnetic fields (AMFs), GSNO can release NO on demand and promote the differentiation and growth of neural stem cells by synergistic stimulation with eddy currents induced by conductive MOFs (Fig. 5a).…”

Section: Mofs Materials In Neural Tissue Engineeringmentioning

confidence: 99%

Metal–organic framework-based platforms for implantation applications: recent advances and challenges

Liu,

Wang,

Quan

et al. 2024

J. Mater. Chem. B

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In situ magnetoelectric generation of nitric oxide and electric stimulus for nerve therapy by wireless chargeable molybdenum carbide octahedrons

Cited by 18 publications

References 47 publications

Revealing intact neuronal circuitry in centimeter-sized formalin-fixed paraffin-embedded brain

Revealing intact neuronal circuitry in centimeter-sized formalin-fixed paraffin-embedded brain

Revealing intact neuronal circuitry in centimeter-sized formalin-fixed paraffin-embedded brain

Metal–organic framework-based platforms for implantation applications: recent advances and challenges

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