In situ (mesocosm) assessment of immunotoxicity risks to small mammals inhabiting petrochemical waste sites

Propst, Timothy L.; Lochmiller, Robert L.; Qualls, Charles W.; McBee, Karen

doi:10.1016/s0045-6535(98)00349-x

Cited by 57 publications

(28 citation statements)

References 33 publications

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“…Oily sludge contains several toxic hydrocarbon constituents, making the sites contaminated by it a major environmental concern because many of the constituents of oily sludge are carcinogenic and potent immunotoxicants (19,22). It is a complex mixture of total petroleum hydrocarbon (TPH), water, and soil particles.…”

mentioning

confidence: 99%

Evaluation of Genetic Diversity among Pseudomonas citronellolis Strains Isolated from Oily Sludge-Contaminated Sites

Bhattacharya

Sarma

Krishnan

et al. 2003

Appl Environ Microbiol

116

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The diversity among a set of bacterial strains that have the capacity to degrade total petroleum hydrocarbons (TPH) in soil contaminated with oily sludge (hazardous hydrocarbon waste from oil refineries) was determined. TPH is composed of alkane, aromatics, nitrogen-, sulfur-, and oxygen-containing compound, and asphaltene fractions of crude oil. The 150 bacterial isolates which could degrade TPH were isolated from soil samples obtained from diverse geoclimatic regions of India. All the isolates were biochemically characterized and identified with a Biolog microbial identification system and by 16S rDNA sequencing. Pseudomonas citronellolis predominated among the 150 isolates obtained from six different geographically diverse samplings. Of the isolates, 29 strains of P. citronellolis were selected for evaluating their genetic diversity. This was performed by molecular typing with repetitive sequence (Rep)-based PCR with primer sets ERIC (enterobacterial repetitive intergenic consensus), REP (repetitive extragenic palindromes), and BOXAIR and PCR-based ribotyping. Strain-specific and unique genotypic fingerprints were distinguished by these molecular typing strategies. The 29 strains of P. citronellolis were separated into 12 distinguishable genotypic groups by Rep-PCR and into seven genomic patterns by PCR-based ribotyping. The genetic diversity of the strains was related to the different geoclimatic isolation sites, type of oily sludge, and age of contamination of the sites. These results indicate that a combination of Rep-PCR fingerprinting and PCR-based ribotyping can be used as a highresolution genomic fingerprinting method for elucidating intraspecies diversity among strains of P. citronellolis.

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confidence: 99%

Evaluation of Genetic Diversity among Pseudomonas citronellolis Strains Isolated from Oily Sludge-Contaminated Sites

Bhattacharya

Sarma

Krishnan

et al. 2003

Appl Environ Microbiol

116

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“…For example, Trust et al (1990) used penraised mallards (Anas platyrhynchos) to investigate the effects of lead ingestion on lymphocyte transformation responses to two mitogens and on antibody titers to SRBC. Similarly, Rocke and Samuel (1991) studied lymphocytes and antibody production to SRBCs in mallards that lived in enclosures with high lead shot density, and Propst et al (1999) assessed the immunotoxicity risk to laboratory-raised cotton rats (Sigmodon hispidus) exposed in situ on a Superfund waste site in Oklahoma to soils contaminated with complex mixtures of organic hydrocarbons and heavy metals. Despite the fact that they found some immune alterations, they concluded that small mammals exposed as long-term residents may be better for biomonitoring than wild animals introduced as mesocosms.…”

Section: Immunologic Assaysmentioning

confidence: 99%

“…There are numerous immunologic assays to evaluate immunocompetence in laboratory and wild animals (Dean and Murray, 1991;Lochmiller et al, 1994;Propst et al, 1999;Grasman and Fox, 2001;Institoris et al, 2001;Smits and Bortolotti, 2001). Measurements of spleen weight and lymphocyte number allow evaluation of the immunopathologic condition of some animals in response to chemical exposure (Dean and Murray, 1991;McMurry et al, 1994;Institoris et al, 2001;Grasman, 2002).…”

Section: Introductionmentioning

confidence: 99%

“…In addition, they cannot reproduce ecologic conditions that influence exposure and uptake of these pollutants in the wild, such as biomagnification and bioaccumulation (McBee and Bickham, 1990). Laboratory studies, therefore, should be complimented by risk assessment studies in natural populations exposed to typical environmental contaminant mixtures and levels (Linzey, 1987;Propst et al, 1999). Ideally, such ecotoxicologic field studies should not only assay the immune response of wild animals, but also provide estimates of contaminant levels of soil and animal tissue.…”

Section: Introductionmentioning

confidence: 99%

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Effects of Heavy Metals on Immunocompetence of White-Footed Mice (Peromyscus Leucopus)

Biser

Vogel

Berger

et al. 2004

Journal of Wildlife Diseases

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Continued human population growth and industrialization result in increased contamination of wildlife habitats. Effects of such habitat deterioration on the well-being of natural populations are unclear. Exposure to contaminants may impair immunocompetence, thereby increasing disease susceptibility. The mammalian immune system is important in maintaining health and in its sensitivity to toxins. In our study conducted from May 1999 through May 2001, we examined assays of immunocompetence in the white-footed mouse (Peromyscus leucopus) that inhabited reference sites and sites significantly contaminated with mixtures of heavy metals. We estimated potential exposure and uptake of heavy metals by measuring the level of each contaminant in representative soil and tissue samples. Intraindividual variation across mice, but not sex, explained a large portion of the overall variance in immune response, and spleen weight was significantly affected by mouse age. We found no evidence that residence on contaminated sites had any effect on immunopathology and humoral immunity as measured in our study. We suggest that field and laboratory studies in ecotoxicology provide estimates of exposure to contaminants (i.e., tissue analyses) to establish a database suitable to clarify the dose-response relationship between contaminants and target systems.

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“…Particularly soil contamination, which poses severe threat to ground water [1]. Some constituents of crude oil are carcinogenic and potential immunotoxics [2]. The impact of crude oil spillage or discharge into the ecosystem breeds environmental concern [3], especially with associated heavy metals present in the crude oil.…”

Section: Introductionmentioning

confidence: 99%

Crude Oil Contaminant and Bio-remediation using Brewery Mash and Earthworm (Nsukkadrilus mbae.) a Consortium to Cleaning-up and Restoring Soil Fertility Potentials

Okpashi¹,

Wallace²,

Dm³

2015

J Pet Environ Biotechnol

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In situ (mesocosm) assessment of immunotoxicity risks to small mammals inhabiting petrochemical waste sites

Cited by 57 publications

References 33 publications

Evaluation of Genetic Diversity among Pseudomonas citronellolis Strains Isolated from Oily Sludge-Contaminated Sites

Evaluation of Genetic Diversity among Pseudomonas citronellolis Strains Isolated from Oily Sludge-Contaminated Sites

Effects of Heavy Metals on Immunocompetence of White-Footed Mice (Peromyscus Leucopus)

Crude Oil Contaminant and Bio-remediation using Brewery Mash and Earthworm (Nsukkadrilus mbae.) a Consortium to Cleaning-up and Restoring Soil Fertility Potentials

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