In-situ mineralized homogeneous collagen-based scaffolds for potential guided bone regeneration

Abstract

For guided bone regeneration (GBR) in clinical orthopedics, the importance of a suitable scaffold which can provide the space needed for bone regeneration and simultaneously promotes the new bone formation cannot be overemphasized. Due to its excellent biocompatibility, mechanical strength, and similarity in structure and composition to natural bone, the mineralized collagen-based scaffolds have been increasingly considered as promising GBR scaffolds. Herein, we propose a novel method to fabricate an in-situ mineralized homogeneous collagen-based scaffold (IMHCS) with excellent osteogenic capability for GBR by electrospinning the collagen solution in combination with essential mineral ions. The IMHCS exhibited homogeneous distribution of apatite crystals in electrospun fibers, which helped to achieve a significantly higher tensile strength than the pure collagen scaffold (CS) and the scaffold with directly added nano-hydroxyapatite particles (HAS). Furthermore, the IMHCS had significantly better cell compatibility, cell migration ratio, and osteogenic differentiation property than the HAS and CS. Therefore, the IMHCS not only retains traditional function of inhibiting fibroblast invasion, but also possesses excellent osteogenic differentiation property, indicating a robust alternative for GBR applications.

3D-printed near-infrared-light-responsive on-demand drug-delivery scaffold for bone regeneration

“…Most of the bone substitutes used for guided bone regeneration in clinical practice are pure collagen, while their mechanical strength is insu cient to withstand forces during regeneration [9,27]. Thus, the collagen/hydroxyapatite-based scaffold composed of inorganic particles has provided a suitable alternative for bone regeneration [28,29].…”

Geometric Angles and Gene Expression in Cells for Structural Bone Regeneration

“…Electrospinning can boost vascularized bone regeneration based on self-features and drug loading. [31] Various multifunctional materials exhibit different capabilities and their properties play decisive roles in bone regeneration. [32] Therefore, we constructed 3D-printed microfibers with different geometric angles using the NFEP technique to investigate the regulatory mechanisms of the geometric angles on stem cell function and bone regeneration.…”

Highly Ordered Nanotube-Like Microstructure on Titanium Dental Implant Surface Fabricated via Anodization Enhanced Cell Adhesion and Migration of Human Gingival Fibroblasts

“…9,10 Also, abutment surface could be physically coated by various biological macromolecules such as chitosan, collagen, and gelatin. [11][12][13] These kinds of natural coating were integrated on the surface to increase protein adhesion and thereafter enhance the attachment of gingival epithelial cells and fibroblasts. However, the uncontrolled degradation rate of these natural components set a challenge to the clinical transformation in the long term.…”