In-situ monitoring of defects in extrusion-based bioprinting processes using visible light imaging

Gugliandolo, Simone Giovanni; Margarita, Alessandro; Santoni, Silvia; Moscatelli, Davide; Colosimo, Bianca Maria

doi:10.1016/j.procir.2022.06.040

Cited by 3 publications

(5 citation statements)

References 22 publications

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“…Other geometry inspection strategies were explored using optical coherence tomography with the possibility of receiving feedback during the fabrication (Yang et al, 2021), (Yang et al, 2023). In (Gerdes et al, 2020), the authors focused on guaranteeing structure integrity using an image-based approach, while in (Gugliandolo et al, 2022), images obtained from a coaxial camera were used to evaluate geometrical defects. Machine learning algorithms were also explored to build a monitoring system capable of detecting printing issue through the classification of images collected using a webcam (Bonatti et al, 2022).…”

Section: On-machine Sensing and Control Strategiesmentioning

confidence: 99%

Biomaterials for extrusion-based bioprinting and biomedical applications

Rossi,

Pescara,

Gambelli

et al. 2024

Front. Bioeng. Biotechnol.

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Amongst the range of bioprinting technologies currently available, bioprinting by material extrusion is gaining increasing popularity due to accessibility, low cost, and the absence of energy sources, such as lasers, which may significantly damage the cells. New applications of extrusion-based bioprinting are systematically emerging in the biomedical field in relation to tissue and organ fabrication. Extrusion-based bioprinting presents a series of specific challenges in relation to achievable resolutions, accuracy and speed. Resolution and accuracy in particular are of paramount importance for the realization of microstructures (for example, vascularization) within tissues and organs. Another major theme of research is cell survival and functional preservation, as extruded bioinks have cells subjected to considerable shear stresses as they travel through the extrusion apparatus. Here, an overview of the main available extrusion-based printing technologies and related families of bioprinting materials (bioinks) is provided. The main challenges related to achieving resolution and accuracy whilst assuring cell viability and function are discussed in relation to specific application contexts in the field of tissue and organ fabrication.

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Section: On-machine Sensing and Control Strategiesmentioning

confidence: 99%

Biomaterials for extrusion-based bioprinting and biomedical applications

Rossi,

Pescara,

Gambelli

et al. 2024

Front. Bioeng. Biotechnol.

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“…Recently, many approaches for in situ monitoring and control of bioprinting processes have been proposed in the literature, exploiting different sensing solutions including cameras in the visible range, [141,142] laser scanners, [143][144][145] and optical coherence tomography (OCT) for a better reconstruction of internal features. [146][147][148] An additional opportunity is the utilization of thermal cameras for monitoring the temperature to support extrusion of sensitive materials [149] or inkjet bioprinting.…”

Section: Online Monitoring Bioreactors and Remote Controlmentioning

confidence: 99%

3D Bioprinting in Microgravity: Opportunities, Challenges, and Possible Applications in Space

Ombergen

Chalupa-Gantner

Chansoria

et al. 2023

Adv Healthcare Materials

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Abstract3D bioprinting has developed tremendously in the last couple of years and enables the fabrication of simple, as well as complex, tissue models. The international space agencies have recognized the unique opportunities of these technologies for manufacturing cell and tissue models for basic research in space, in particular for investigating the effects of microgravity and cosmic radiation on different types of human tissues. In addition, bioprinting is capable of producing clinically applicable tissue grafts, and its implementation in space therefore can support the autonomous medical treatment options for astronauts in future long term and far‐distant space missions. The article discusses opportunities but also challenges of operating different types of bioprinters under space conditions, mainly in microgravity. While some process steps, most of which involving the handling of liquids, are challenging under microgravity, this environment can help overcome problems such as cell sedimentation in low viscous bioinks. Hopefully, this publication will motivate more researchers to engage in the topic, with publicly available bioprinting opportunities becoming available at the International Space Station (ISS) in the imminent future.

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“…Camera systems have been previously employed to monitor bioprinting outcomes, but typically at oblique angles and/or larger distances from the extrusion nozzle 10,11,[14][15][16] . Our unique axis cloning approach positions the camera directly beneath the extrusion nozzle during the bioprinting process, enabling highresolution imaging and tracking of bioink flow.…”

Section: Main Development Of Bioprinting Hardware With In-situ Qualit...mentioning

confidence: 99%

“…Early work in the field has shown the potential of using camera systems to monitor extrusion bioprinting outcomes [10][11][12] . Such approaches can be used for in-situ assessment of print quality on the bioprinting platform.…”

Section: Introductionmentioning

confidence: 99%

“…The control strategy was capable of intelligently updating the extrusion inputs in the g-code to improve accuracy for future layers 12 . While promising, cameras have typically been located at relatively large distances or oblique angles from the extrusion nozzle [10][11][12][14][15][16] . This limits image resolution and prevents monitoring of bioink flow, filament formation, and small features within the bioprinted construct.…”

Section: Introductionmentioning

confidence: 99%

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In-situ quality monitoring during embedded bioprinting using integrated microscopy and classical computer vision

Britchfield

Daly

2022

Preprint

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Embedded bioprinting into support hydrogels enables controlled extrusion of low viscosity bioinks into complex 3D structures with high-resolution features. Granular hydrogels, formed through packing of hydrogel microparticles, are increasingly employed as support materials due to their shear-thinning and self-healing properties that are essential for the embedded bioprinting process. Despite the widespread use of granular support hydrogels for embedded bioprinting, their general design criteria such as the optimal particle morphology, size, and packing density have not been established. Here, we developed granular support hydrogels with varied particle morphologies and packing densities, to explore how these parameters can influence rheological behaviour and the embedded bioprinting process. Interestingly, lower viscosity support hydrogels with a higher yield point, formulated from particles with irregular morphologies that increased particle-particle interactions, were found to improve print quality outcomes. In particular, we observed these formulations improved extrusion consistency during complex print paths and reduced disruption of adjacent filaments during successive print paths. These results improve our understanding of particle-particle interactions in granular support hydrogels during embedded bioprinting, and can be used to inform the design of future support hydrogel compositions.

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In-situ monitoring of defects in extrusion-based bioprinting processes using visible light imaging

Cited by 3 publications

References 22 publications

Biomaterials for extrusion-based bioprinting and biomedical applications

Biomaterials for extrusion-based bioprinting and biomedical applications

3D Bioprinting in Microgravity: Opportunities, Challenges, and Possible Applications in Space

In-situ quality monitoring during embedded bioprinting using integrated microscopy and classical computer vision

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