In situ preparation of nanohydroxyapatite/alginate composites as additives to PVA electrospun fibers as new bone graft materials

Correia, Gregório; Falcão, Juliana S. A.; Neto, Antônio Gomes de Castro; Silva, Y.J.A.; Mendonça, Leonardo Tadeu Boaes; Barros, Alessandro; Santos-Oliveira, Ralph; Azevêdo, W.M. de; Alves, Sara Kely Learsi da Silva Santos; Santos, Beate S.

doi:10.1016/j.matchemphys.2022.125879

Cited by 8 publications

(2 citation statements)

References 54 publications

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“…(a) (b) Figure 4 shows the SEM micrograph and the diameter histogram of the fibers of the composite membrane. In Figure 4a, the SEM micrograph depicts a membrane with fibrous structures exhibiting a random arrangement with irregular diameters and spacings of different sizes between fibers without showing coalescence [35]. The surface of fibers is completely smooth and reveals the presence of nodules that can be attributed to the presence of PVA in the membrane and could be related to the highest concentration of the starting solution compared to the PLA solution.…”

Section: Polymeric Electrospinning Membranesmentioning

confidence: 99%

Fabrication of a PLA/PVA-BIO-HA Polymeric Membrane by the Electrospinning Technique

Arroyo-Reyes,

Gómez-Muñoz,

Zaca-Morán

et al. 2024

Fibers

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In the present work, the fabrication of a membrane composed of polylactic acid (PLA), polyvinyl alcohol (PVA), and Biological Hydroxyapatite (BIO-HA) is reported using the coaxial electrospinning technique. The membrane fabrication process involved mixing a solution of PLA and trichloromethane (TCM) with a second solution of PVA, isopropyl alcohol (IPA), distilled water, and BIO-HA at 110 °C. Subsequently, the electrospinning process was carried out using a voltage of 25 kV for 30 min on a rotating drum collector at 1000 rpm. The membrane was characterized through Scanning Electron Microscopy (SEM), Energy-Dispersive X-ray spectroscopy (EDS), Fourier-Transform Infrared spectroscopy (FTIR), Thermogravimetric Analysis (TGA), and Differential Scanning Calorimetry (DSC). The morphological results revealed the presence of randomly arranged fibers with an average diameter of 290 ± 9 nm and interfiber spacing ranging from 200 to 700 nm, which are characteristics conducive to cell proliferation. Additionally, FTIR studies confirmed the presence of BIO-HA and the constituent elements of the polymers in the composite membrane. The polymeric membrane in contact with human mesenchymal stem cells was characterized as showing significant differences in its behavior at 6, 24, and 72 h post-contact. These studies indicate that the membrane provides physical support as a scaffold due to its suitable morphology for cell adhesion and proliferation, attributable to the electrospinning conditions as well as the polymers contained in BIO-HA. Membrane toxicity was confirmed through a cytotoxicity study using fluorescence microscopy, which showed that the membrane provided a favorable environment for cell proliferation. These results suggest that exposure to BIO-HA enhances its potential application in bone and joint tissue regeneration.

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Section: Polymeric Electrospinning Membranesmentioning

confidence: 99%

Fabrication of a PLA/PVA-BIO-HA Polymeric Membrane by the Electrospinning Technique

Arroyo-Reyes,

Gómez-Muñoz,

Zaca-Morán

et al. 2024

Fibers

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“…One of the main challenges for preparing polymer and nHAp composites as bone scaffolds is physicochemical homogeneity. Correia et al [54] developed a new procedure to fabricate a nanocomposite composed of in situ growth of nHAp coated with alginate (ALG), overcoming the problem. Then, they mixed nHAp/ALG nanocomposites with polyvinyl alcohol (PVA) to obtain nanofiber matrix scaffolds with electrospinning.…”

Section: Naturally Derived Materialsmentioning

confidence: 99%

The Influence of Scaffold Interfaces Containing Natural Bone Elements on Bone Tissue Engineering Applications

Vadivelmurugan

Tsai

2022

Coatings

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Bone has the capacity to repair damage and reproduce itself, but if the defect is too large, a scaffold is needed to promote regeneration. Recently, researchers have developed numerous approaches to promote bone reformation, such as the direct delivery of bioactive molecules, guided tissue regeneration membranes, and creating osteoinduction/osteoconduction surfaces. The surface of a medical device is the first contact area for a biological system; therefore, the interactions between biological fluids and the surface of the implant determine the performance of the implant. Well-designed surface physical and chemical properties, such as topography, net charge, components, and hydrophilicity, enhance cell attachment and proliferation. Various surface modification technologies and methods have been studied to enhance cellular expression. This review selects scaffold materials that are FDA-approved and have been widely used in the clinic and focuses on recent studies of surface modification with hydroxyapatites and collagen, which are the main components of the bone matrix, for the enhancement of bone regrowth.

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Enhancing the Strength of Microporous Nanofibrous PVA Mats by Incorporating Nanostructured Hydroxyapatite Synthesized by a Combustion Route for Bone Tissue Regeneration

Swapna

Mathew

Thomas

2023

J Inorg Organomet Polym

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In situ preparation of nanohydroxyapatite/alginate composites as additives to PVA electrospun fibers as new bone graft materials

Cited by 8 publications

References 54 publications

Fabrication of a PLA/PVA-BIO-HA Polymeric Membrane by the Electrospinning Technique

Fabrication of a PLA/PVA-BIO-HA Polymeric Membrane by the Electrospinning Technique

The Influence of Scaffold Interfaces Containing Natural Bone Elements on Bone Tissue Engineering Applications

Enhancing the Strength of Microporous Nanofibrous PVA Mats by Incorporating Nanostructured Hydroxyapatite Synthesized by a Combustion Route for Bone Tissue Regeneration

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