In situ reconstruction with bovine pericardial tubular graft for aortic graft infection

Dulbecco, Eduardo; Camporrotondo, Mariano; Blanco, Gustavo; Haberman, Diego

doi:10.1590/s0102-76382010000200020

Cited by 13 publications

(5 citation statements)

References 17 publications

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“…However, extraanatomical reconstruction is not feasible in all cases (eg, in case of ascending aortic graft infection or aortic arch graft infection) and shows variable outcome with regard to long-term durability in more distal segments [7]. In fact, patency rates of axillofemoral bypasses have been reported to be lower than 50% after 5 years, and late rupture is not uncommon [8]. Moreover, even in cases of extraanatomical reconstruction, the artificial surface of the prosthetic graft is in high risk of reinfection in the presence of bacteremia.…”

Section: Commentmentioning

confidence: 99%

Surgical Treatment of Native and Prosthetic Aortic Infection With Xenopericardial Tube Grafts

Kreibich

Siepe

Morlock

et al. 2018

The Annals of Thoracic Surgery

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Patients with infectious aortic disease present in a frail state and the initial and the late mortality is substantial. Yet, surgical treatment of native and prosthetic aortic infection with xenopericardial tube grafts shows promising short-term results with regard to durability and freedom from reinfection in any aortic segment. Further studies are needed to learn of the long-term behavior of these grafts.

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Section: Commentmentioning

confidence: 99%

Surgical Treatment of Native and Prosthetic Aortic Infection With Xenopericardial Tube Grafts

Kreibich

Siepe

Morlock

et al. 2018

The Annals of Thoracic Surgery

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“…Nowadays, the utilization of biological xenografts (graft originates from another species) shows an increasing number, as a result of the growing demands in vascular surgery, decisively in the treatment of septic vascular prostheses [11][12][13][14]. However, frequent, late aneurysm formation due to their degeneration is the most unfavorable consequence of their use.…”

Section: Resultsmentioning

confidence: 99%

DSC and histological analysis of decellularized porcine biograft

Fazekas

Jancsó

Lőrinczy

2020

J Therm Anal Calorim

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Different types of biological grafts are useful for the replacement of infected prostheses in vascular surgery. In cases, when autologous grafts or homografts are not available, xenografts can be an alternative choice. To reduce immunogenicity, decellularization of the xenogeneic graft is necessary. Our goal was to determine the long-lasting stability of our decellularized porcine vascular grafts, in the mirror of differential scanning calorimetry (DSC) and routine histological examination. In the first experiment, light and electron microscopic confirmation of adequate decellularization of our used protocol was assessed. In the second series, DSC measurements were performed both on native and on treated samples; thereafter, histological evaluation of the analyzed samples was performed. Light and electron microscopy evaluation showed complete removal of cells and preserved connective tissue fiber structure after decellularization in the first series. In the second part of our investigation, DSC measurements demonstrated reversible change in the structure of elastic fibers and irreversible change in the structure of collagen fiber structure supported by histological findings. Our examination strengthens the earlier observations that decellularized biological grafts can tend to late aneurysmatic degeneration, due to their weakened fiber structure, besides the known immunological circumstances.

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“…Freedom from reinfection proved 100% within a 24-month follow-up period. Dulbecco et al [22] made a successful reconstruction with a bovine pericardial tubular aortobifemoral graft in case of an aortic graft infection. Lutz et al [19] used a hand-sewn bovine pericardial xenoprosthesis for in situ graft replacement of an infected descending or infrarenal aortic graft.…”

Section: Discussionmentioning

confidence: 99%

Histological and Mechanical Assessment of Decellularized Porcine Biografts, and Its Biological Evaluation following Aortic Implantation during Mid-Term Follow-Up

et al. 2018

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Aims: Prosthetic graft infection frequently requires graft replacement. Among other options, a biological graft could serve as an alternative choice. Decellularization reduces tissue immunogenicity. Our aim was to determine an efficient decellularization method and to evaluate the decellularized porcine biografts’ adaptability. Methods: Four different protocols were implemented to decellularize porcine aortic segments (n = 4). Cell removal effectiveness and matrix structure preservation were histologically examined. Mechanical tests were performed. Decellularized porcine grafts were interpositioned in a porcine aorta. After a 6-month period, implanted samples were removed and evaluated using light and electron microscopy. Results: Histological results showed complete removal of cells and preserved connective tissue fiber structure following decellularization, using sodium dodecyl sulfate and sodium azide. Pressure tests demonstrated similar compliance to fresh vessels. In 9 out of 10 cases, pigs survived the follow-up period. Graft rejection, intimal hyperplasia, reocclusion and/or aneurysm formation were not observed. Presence of host cells and neoendothelialization were microscopically confirmed. Conclusions: This decellularization protocol enables a cost-effective preparation of biological grafts featuring reduced immunogenicity. The implanted grafts did not degenerate during the 6-month follow-up period, the lack of graft rejection suggests acceptable immunological tolerance, while recipient cells migrate into, proliferate and differentiate, thus creating the possibility for further use as an optional vascular graft.

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In situ reconstruction with bovine pericardial tubular graft for aortic graft infection

Cited by 13 publications

References 17 publications

Surgical Treatment of Native and Prosthetic Aortic Infection With Xenopericardial Tube Grafts

Surgical Treatment of Native and Prosthetic Aortic Infection With Xenopericardial Tube Grafts

DSC and histological analysis of decellularized porcine biograft

Histological and Mechanical Assessment of Decellularized Porcine Biografts, and Its Biological Evaluation following Aortic Implantation during Mid-Term Follow-Up

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