In situ sensing of metal ion adsorption to a thiolated surface using surface plasmon resonance spectroscopy

Moon, Jei‐Kwon; Kang, Taewook; Oh, Seogil; Hong, Seung‐Tae; Yi, Jongheop

doi:10.1016/j.jcis.2005.12.066

Cited by 35 publications

(17 citation statements)

References 30 publications

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“…This agrees with J. Moon et al whose work showed a right shift in the position of minimum reflectance in a thiolate (hexanedecanethiol) surface as compared to that of bare Au using SPR [10].…”

Section: Shift In Resonant Dip Observed With Temperaturesupporting

confidence: 91%

A thermal and passivation study of self-assembled monolayers on thin gold films

Ang

Wei

et al. 2008

Thin Solid Films

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Section: Shift In Resonant Dip Observed With Temperaturesupporting

confidence: 91%

A thermal and passivation study of self-assembled monolayers on thin gold films

Ang

Wei

et al. 2008

Thin Solid Films

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“…Variations in ionic concentrations have been demonstrated to influence the SPR signal in a wide variety of experiments 9,28. In a living cell system, the intracellular ion concentration in the vicinity of the sensing surface could also potentially contribute to the SPR signal by causing a mass variation within the evanescent field.…”

Section: Discussionmentioning

confidence: 99%

Surface Plasmon Resonance Monitoring of Cell Monolayer Integrity: Implication of Signaling Pathways Involved in Actin-Driven Morphological Remodeling

et al. 2008

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Morphological changes occurring in individual cells largely influence the physiological functions of various cell layers. The control of barrier function of epithelia and endothelia is a prime example of processes highly dependent on cellular morphology and cell layer integrity. Here, we applied the surface plasmon resonance (SPR) technique to the quantification of cellular activity of an epithelial cell monolayer stimulated by angiotensin II. The analysis of the SPR signal shows reproducible concentration-dependent biphasic responses after cell activation with angiotensin II. Phase-contrast and confocal microscopy imaging was performed to link the SPR signal to molecular and global morphological remodeling. The SPR signal was observed to be in relation with the rapid cell contraction and the subsequent cell spreading observed by phase-contrast microscopy. Additionally, the temporal redistribution of actin, observed by confocal microscopy after angiotensin II stimulation, was also found to be consistent with the SPR signal variation. The modulation of signaling pathways involved in actin-myosin driven cell contraction confirms the direct implication of actin structures in the SPR response. Additionally, we show that the intracellular calcium mobilization associated with angiotensin II stimulation did not produce any significant SPR signal variation. Altogether, our results demonstrate that SPR is a rapid label-free method to study cellular activity and molecular mechanisms implicated in the modulation of the integrity of a cell monolayer in relation to cytoskeleton remodeling with associated cell morphological changes.

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“…AgNPs are wellknown for their localized surface plasmon resonance (LSPR) properties, which originate from collective oscillation of their electrons in response to optical excitation. 18 In general, spherical AgNPs with a size of about 10 nm size have an LSPR peak around 400 nm. Therefore, a maximum peak in deviation spectra is induced by this LSPR phenomenon.…”

Section: Resultsmentioning

confidence: 99%

Simple Analysis for Interaction between Nanoparticles and Dye-Containing Vesicles as a Biomimetic Cell-Membrane

Shin

Umh²,

Kim³

2013

Bulletin of the Korean Chemical Society

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Some cytotoxicity studies for the interpretation of the interaction between nanoparticles and cells are nonmechanistic and time-consuming. Therefore, non-biological screening methods, which are faster and simpler than in-vivo and in-vitro methods, are required as alternatives to current cytotoxicity tests. Here, we proposed a simple screening method for the analysis of the interaction between several AgNPs (bare-, citrate-, and polyvinylpyrrolidone-coating) and dye-containing vesicles acting as a biomimetic cell-membrane. The interaction between AgNPs and vesicles could be evaluated readily by UV-vis spectra. Absorbance deviation in UV-vis spectra revealed a large attraction between neighboring particles and vesicles. This was confirmed by (Derjagin, Landau, Verwey, and Overbeek) theory and DMF (dark-field microscopy) analysis. This proposed method might be useful for analyzing the cytotoxicity of nanoparticles with cell-membranes instead of in vitro or in vivo cytotoxicity tests.

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In situ sensing of metal ion adsorption to a thiolated surface using surface plasmon resonance spectroscopy

Cited by 35 publications

References 30 publications

A thermal and passivation study of self-assembled monolayers on thin gold films

A thermal and passivation study of self-assembled monolayers on thin gold films

Surface Plasmon Resonance Monitoring of Cell Monolayer Integrity: Implication of Signaling Pathways Involved in Actin-Driven Morphological Remodeling

Simple Analysis for Interaction between Nanoparticles and Dye-Containing Vesicles as a Biomimetic Cell-Membrane

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