In situ silver nanoparticle formation embedded into a photopolymerized hydrogel with biocide properties

Abstract

In situ nanoparticle formation embedded into hydrogel matrix, acting as container and stabilizer for nanoparticle reaction was the focus in this research; this method was realized using AgNO 3 (0.75 and 1.0 M) as silver source for nanoparticle formation; also, monomers (HEMA), cross-linker agents (DEGDMA) and a photoinitiator (Irgacure 651) were used for the hydrogel synthesis. For the reduction of Ag ? ? Ag 0 , the reaction mixture was irradiated with an UV lamp at 365 nm for 30 min; parallel to this process, the hydrogel photopolymerization occurs. All these systems were studied by Infrared and Raman Spectroscopy, optical studies: UV/Vis absorption, thermal studies: differential scanning calorimetry and thermogravimetric analysis, X-ray diffraction, fluorescence X-ray spectroscopy and transmission electronic microscopy. Characterization techniques are capable to detect the presence of non-agglomerated silver nanoparticles homogeneously distributed in all the systems. X-Ray photoemission spectroscopy establishes the presence of Ag 0 and Ag ? as mixture in the synthesized composite. Quantitative assays show that the sample Ag_Hg3-89.5 % (1.0 M) presents an important biocide property, by reducing 99.9 % of bacterium Escherichia coli ATCC 25922 as compared with the alone hydrogel used as control.

Novel In-situ Precipitation Process to Engineer Low Permeability Porous Composite

“…For example, gold nanoparticles are precipitated on the surface of a glass substrate for architectural applications 1 . Another example is silver nanoparticles are precipitated in a polymer hydrogel to develop biocidal properties 6 . The precipitation of inorganic particles occurs through nucleation growth induced by the super saturation 7 or mixing 8 , 9 of solvent or reagent.…”

Colloids-at-surfaces: Physicochemical approaches for facilitating cell adhesion on hybrid hydrogels

“…Carbon-based nanoparticles (carbon nanotubes), [86][87][88] polymer nanoparticles (hyper-branched polymers), [19,89] inorganic particles (silicates), [90,91] ceramic nanoparticles (hydroxyappatite and calcium phosphate), [92,93] metal nanoparticles (gold, silver and iron) [94,95] are widely used to modify hydrogels: 1) response to various stimuli such as light, [96] electricity, [97] magnetic field, [98] ultrasound; [99] 2) improvement of mechanical properties; [21] 3) creating a three-dimensional relief on the surface; 4) improved cell adhesion. [100] Spark et al studied the behavior of cells on a nanostructured material with emphasis on the fundamental adhesive properties of cells.…”

Advances in the Fabrication of Antimicrobial Hydrogels for Biomedical Applications

“…In addition, Gonzalez-Henriquez et al [ 53 , 54 ] also generated in situ nanoparticles embedded into a hydrogel matrix acting as a container and stabilizer during the nanoparticle formation. This method was realized using AgNO 3 as the silver source.…”