In‐situ sorbent formation for the extraction of pesticides from honey

Abstract

An organic polymer was re‐precipitated in solution to use as an adsorbent in dispersive solid‐phase extraction of some pesticides from honey samples prior to their determination by high‐performance liquid chromatography‐tandem mass spectrometry. In this approach, different deep eutectic solvents were prepared using lysine and their ability in elution of the analytes from the adsorbent surface was tested. A diluted honey solution was transferred into a glass test tube and then a solution of polystyrene dissolved in dimethylformamide was injected into the solution. By doing this, polystyrene is re‐precipitated in the solution and dispersed in whole parts of it as many tiny particles. Then the mixture was centrifuged and the adsorbed analytes on the particles were eluted using a proper hydrophilic deep eutectic solvent. The central composite design approach was used for the optimization of effective parameters. The limits of detection and quantification were in the ranges of 0.06–0.20 and 0.22–0.69 ng/g, respectively. The calibration curves obtained by matrix‐matched standard solutions were linear in the range of 0.69–500 ng/g with a coefficient of determinations ≥0.9962. The method provided high extraction recoveries (70–99%) and enrichment factors (140–198), and an acceptable precision (relative standard deviations ≤7.1%).

HPLC-FLD determination of aflatoxins M1 and M2 in raw cow milk samples using in-syringe gas-controlled density tunable solidification of a floating organic droplet-based dispersive liquid–liquid microextraction method

“…19,20 DESs are prepared through a reaction between a hydrogen-bond donor (HBD) and a hydrogen-bond acceptor (HBA) at an elevated temperature. 21 The melting point of the formed solvent is lower than that of its components. 22 The key aim of this research was to validate and apply an insyringe gas-controlled density tunable SFOD-DLLME method for the efficient extraction of AFM 1 and AFM 2 from cow milk samples.…”