In situ synthesis of biomolecule encapsulated gold-cross-linked poly(ethylene glycol) nanocomposite as biosensing platform: A model study

Abstract

In situ synthesis of poly(ethylene glycol) (PEG) hydrogels containing gold nanoparticles (AuNPs) and glucose oxidase (GOx) enzyme by photo-induced electron transfer process was reported here and applied in electrochemical glucose biosensing as the model system. Newly designed bionanocomposite matrix by simple one-step fabrication offered a good contact between the active site of the enzyme and AuNPs inside the network that caused the promotion in the electron transfer properties that was evidenced by cyclic voltammetry as well as higher amperometric biosensing responses in comparing with response signals obtained from the matrix without AuNPs. As well as some parameters important in the optimization studies such as optimum pH, enzyme loading and AuNP amount, the analytical characteristics of the biosensor (AuNP/GOx) were examined by the monitoring of chronoamperometric response due to the oxygen consumption through the enzymatic reaction at -0.7 V under optimized conditions at sodium acetate buffer (50 mM, pH 4.0) and the linear graph was obtained in the range of 0.1-1.0 mM glucose. The detection limit (LOD) of the biosensor was calculated as 0.06 mM by using the signal to noise ratio of 3. Moreover, the presence of AuNPs was visualized by TEM. Finally, the biosensor was applied for glucose analysis for some beverages and obtained data were compared with HPLC as the reference method to test the possible matrix effect due to the nature of the samples.

Nanocomposite Hydrogels and Their Applications in Drug Delivery and Tissue Engineering

“…By utilizing this method, biosensor based on PEG NC gels containing gold nanoparticles and glucose oxidase enzyme were fabricated in one pot. 29 The reduction of metal salts and the polymerization of precursor can also been carried out in one-pot through -radiation technique. 30 Carbon Nanotubes and Graphene Nanocomposite Hydrogel Carbon nanotubes (CNTs) have attracted great interest since they were first reported in 1991.…”

Enzyme immobilization in biosensor constructions: self-assembled monolayers of calixarenes containing thiols

“…2A, respectively. A reversible electrochemical response for Fe(CN) 6 3À was obtained. Well-dened oxidation and reduction peaks are observed at about 0.258 and 0.165 V for bare electrode (peak-to-peak separation of 93 mV), 0.244 and 0.151 V for SHCalix modied electrode (peak-to-peak separation of 93 mV) and 0.338 and 0.139 V for Calix-SH/GOx-modied Au electrodes (peak-to-peak separation of 199 mV).…”

“…Aer fabrication of Calix-SH/GOx biosensor, initially the obtained biomembrane was characterized. To evaluate of the conductivity of the biomolecule-modied gold electrode, Calix-SH/GOx biosensor was characterized by cyclic voltammetry (CV) using Fe(CN) 6 3À as an electrochemical probe. CV obtained at the bare, SH-Calix and SH-Calix/GOx modied Au electrodes immersed in aqueous 0.1 M KCl containing 5 mM Fe(CN) 6 3À is shown in Fig.…”

Carbon Nanotube Modified Screen Printed Electrodes: Pyranose Oxidase Immobilization Platform for Amperometric Enzyme Sensors

“…It catalyses the oxidation reaction of D-glucose (also several aldopyranoses) at C-2 position using oxygen as a co-substrate and produces 2-keto sugars and H2O2. As PyOx catalyses oxidation of sugars at the C-2 position (glucose oxidase, GOx, at C-1) [20][21][22][23]. PyOx recognizes both anomeric forms of glucose, and β-form, as substrates.…”