In situ synthesis of water dispersible bovine serum albumin capped gold and silver nanoparticles and their cytocompatibility studies

Murawala, Prayag; Phadnis, Supriya; Bhonde, Ramesh; Prasad, B. L. V.

doi:10.1016/j.colsurfb.2009.05.029

Cited by 76 publications

(58 citation statements)

References 39 publications

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“…Similarly 20-nm BSA-decorated AuNPs towards HeLa cultures were without any cytotoxic effect [27]. A similar behavior was detected by LDH testing with 15-nm BSA-decorated AuNPs coated with BSA to 3T3/NIH cells by a MTT test [28].…”

Section: On Biodecorated Gold Nanoparticles Distributed Within Tissuesupporting

confidence: 68%

On Biodecorated Gold Nanoparticles Distributed within Tissues and Cells

Capek¹

2015

JNMR

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Vocabulary EndocytosisIt is an energy-using process by which cells absorb molecules (such as proteins) by engulfing them. It is used by all cells of the body because most substances important to them are large polar molecules that cannot pass through the hydrophobic plasma or cell membrane. The opposite process is exocytosis. Lactate dehydrogenase (LDH)It is of medical significance because it is found extensively in body tissues, such as blood cells and heart muscle. Because it is released during tissue damage, it is a marker of common injuries and disease. The MTT assayIt is a colorimetric assay for assessing cell viability. WSTs (water soluble tetrazolium salts)These are a series of other water soluble dyes for MTT assays, developed to give different absorption spectra of the formed formazans. WST-1 is advantageous over MTT in that it is reduced outside cells. WST assays (1) can be read directly (unlike MTT that needs a solubilization step), give a more effective signal than MTT, and decrease toxicity to cells (unlike cell-permeable MTT, and its insoluble formazan that accumulate inside cells). BiotinIt also known as vitamin H or coenzyme R, is a water-soluble B-vitamin (vitamin B7). Biotin has an unusual structure, with two rings fused together via one of their sides. The two rings are ureido and thiophene moieties. Biotin is a heterocyclic, S-containing monocarboxylic acid.

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Section: On Biodecorated Gold Nanoparticles Distributed Within Tissuesupporting

confidence: 68%

On Biodecorated Gold Nanoparticles Distributed within Tissues and Cells

Capek¹

2015

JNMR

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“…8 Biological synthesis of nanoparticles using plant extracts, plant products, bacteria, yeast, and fungi has been suggested as a valuable alternative tool toward traditional chemical synthesis methods. [9][10][11][12] Recently, synthesis of gold nanoparticles (ANPs) has been the focus of intense scientific research owing to their fascinating chemical, electronic, and optical properties and potential applications in biomedicine, sensing, and catalysis. 13,14 The ANPs are a promising candidate for biomedical engineering and drug delivery applications owing to their unique dimensions, nontoxicity, surface functionalities, and capacity for controlled drug release.…”

Section: Introductionmentioning

confidence: 99%

Novel green synthesis of gold nanoparticles using Citrullus lanatus rind and investigation of proteasome inhibitory activity, antibacterial, and antioxidant potential

Patra¹,

Baek²

2015

IJN

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Biological synthesis of nanoparticles using nontoxic, eco-friendly approaches is gaining importance owing to their fascinating biocompatibility and environmentally benign nature. This study describes the green synthesis approach for synthesis of gold nanoparticles (ANPs) using aqueous extract of the rind of watermelon as a fruit waste and evaluate its biopotential in terms of proteasome inhibitory activity, antibacterial, and antioxidant potential. The synthesized ANPs were characterized using UV–vis spectroscopy, scanning electron microscopy, X-ray diffraction, Fourier-transform infrared spectroscopy, and thermogravimetric analysis. The surface plasmon resonance spectra of ANPs were obtained at 560 nm. Scanning electron microscopy image revealed that particles had a spherical shape and have a size distribution of 20–140 nm, followed by the elemental analysis by energy-dispersive X-ray spectroscopy. X-ray diffraction analysis confirmed the crystallite nature of the ANPs and Fourier-transform infrared spectroscopy revealed the involvement of bioactive compounds from watermelon rind in the synthesis, capping, and stabilization of ANPs. ANPs exhibited potential antibacterial activity against five different foodborne pathogenic bacteria with diameter of inhibition zones ranged between 9.23 and 11.58 mm. They also displayed strong synergistic antibacterial activity together with kanamycin (11.93–21.08 mm inhibition zones) and rifampicin (10.32–24.84 mm inhibition zones). ANPs displayed strong antioxidant activity in terms of DPPH radical scavenging (24.69%), nitric oxide scavenging (25.62%), ABTS scavenging (29.42%), and reducing power. Significantly high proteasome inhibitory potential of the ANPs (28.16%) could be highly useful for cancer treatment and targeted cancer drug delivery. Overall, results highlight a potential low-cost green method of synthesizing ANPs from food waste materials. Significant biopotentials of synthesized ANPs could make it a potential candidate for its application in the biomedical, pharmaceutical, cosmetics, and food sectors.

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“…In contrast, metal nanoparticles synthesized using biological methods have shown excellent stability, for example, Ag nanoparticles synthesized using bovine serum albumin as a reducing agent showed excellent stability under extreme pH and salt conditions. 8 Furthermore, biosynthesis methods are considered green approaches and have attracted substantial interest. In this section on the recent developments in metal nanoparticle synthesis, we discuss Cu nanoparticle synthesis, shape-controlled biosynthesis of metal nanoparticles using cell extracts and plasmid DNA, sitespecific metal nanoparticle placement using bacterial membranes as masks and Au nanoparticle synthesis.…”

Section: Metal Nanoparticle Synthesismentioning

confidence: 99%

“…Mechanisms for reducing metal ions usually involve donating electrons from the metabolic products, for example, the thiol protein, thiol amino acid, cytochrome, DNA, RNA, NADPH and other various reductases. For example, the reduction process that forms gold nanoparticles in the biosystems receives electrons from proteins 8,14,15 or NADPH coupled with glutathione. 17,18 The Ag nanoparticle preparations were observed in biosynthesis via electron donation from the enzyme-mediated reducing process, 9 plasmid scaffolded and reduced process, 11 and cytochrome-supporting reduction.…”

Section: Concluding Remarks and Prospectsmentioning

confidence: 99%

Nanoparticle biosynthesis using unicellular and subcellular supports

2015

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Modern life is becoming increasingly sophisticated because of products engineered using designs inspired by nature. Fifty years ago, the burdock plant motivated Swiss scientists to invent Velcro, which was a simple but widely applied design that is still considered the greatest biomimetic invention yet. In nanotechnology, interest in biosynthesis of nanoparticles is increasing, particularly in the use of unicellular and subcellular supports. However, the polydispersity of the resultant structure, limited opportunity for product control and commercial applications of biosynthesized nanoparticles remain as challenges. Using different approaches, studies have attempted to understand nanoparticle biosynthesis and control of particle size and uniformity. In the biotechnological approach, gene sequences that were identified in one organism via gene silencing and were critical for nanoparticle synthesis were introduced and expressed in a different organism or overexpressed by promoters in the same organism to enhance productivity. In contrast, physical and chemical approaches were used for the control of nanoparticle synthesis. To provide a comprehensive understanding of nanoparticle biosynthesis using unicellular and subcellular supports, this review discusses recent studies and experimental evidence in the following categories: synthesis of metal, quantum dot, magnetic and bacterial protein nanoparticles.

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In situ synthesis of water dispersible bovine serum albumin capped gold and silver nanoparticles and their cytocompatibility studies

Cited by 76 publications

References 39 publications

On Biodecorated Gold Nanoparticles Distributed within Tissues and Cells

On Biodecorated Gold Nanoparticles Distributed within Tissues and Cells

Novel green synthesis of gold nanoparticles using Citrullus lanatus rind and investigation of proteasome inhibitory activity, antibacterial, and antioxidant potential

Nanoparticle biosynthesis using unicellular and subcellular supports

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