1997
DOI: 10.1006/abio.1997.2097
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In SituNitric Oxide (NO) Measurement by Modified Electrodes: NO Labilized by Photolysis of Metal Nitrosyl Complexes

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Cited by 81 publications
(32 citation statements)
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“…Among these is sodium nitroprusside Na 2 [Fe(CN) 5 NO], which has long been used therapeutically as a vasodilator during hypertensive emergencies [69]. Although photoactive [70,71], nitroprusside can also release NO thermally upon contact with tissue containing reducing species thereby limiting the photochemical control over the release of NO in this case.…”
Section: Iron Complexesmentioning
confidence: 99%
“…Among these is sodium nitroprusside Na 2 [Fe(CN) 5 NO], which has long been used therapeutically as a vasodilator during hypertensive emergencies [69]. Although photoactive [70,71], nitroprusside can also release NO thermally upon contact with tissue containing reducing species thereby limiting the photochemical control over the release of NO in this case.…”
Section: Iron Complexesmentioning
confidence: 99%
“…The sensitivity of this apparatus ranges between 1 nmol/L and 20 μmol/L, with a 2-mm sensor, which directly detects NO concentration by amperometric technique. The setup was calibrated following the procedure previously reported [22][23][24]. The recordings were performed in organ chamber with aortic rings in Krebs solution maintained at pH 7.4 gassed with 95 % O 2 and 5 % CO 2 at 37°C under ambient light to guarantee similar conditions of the functional studies.…”
Section: Methodsmentioning
confidence: 99%
“…The fluorescence intensity of DAF-2-T was converted to concentration of NO on the basis of the calibration curve method: an aliquot of a stock solution of DAF-2 in DMSO (40 mL, 5 mm) and DMSO (320 mL) were diluted by adding PBS buffer (20.44 mL, pH 7.4; PBS-DMSO (containing 1.7 % DMSO)). An aliquot of saturated PBS stock solution (2.5, 5.0, 10, or 20 mL, 1.9 mm) of NO (gas) [15] was added to an aliquot of the above DAF-2 solution (2600 mL, 9.62 mm) in a glass vial equipped with a magnetic stirrer. The final concentrations of NO were 1.9, 3.8, 7.6, and 15.2 mm, respectively.…”
Section: Daf-2 Assaymentioning
confidence: 99%