In-straw warming protocol improves survival of vitrified embryos and allows direct transfer in cattle

Oliveira, C. S.; Feuchard, Viviane Luzia da Silva; Freitas, Célio de; Rosa, Paola Maria da Silva; Camargo, Agostinho Jorge dos Reis; Saraiva, Naiara Zoccal

doi:10.1016/j.cryobiol.2020.02.007

Cited by 17 publications

(9 citation statements)

References 6 publications

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“…As we also observed, the one-step in-straw warming/dilution of expanded blastocysts vitrified in fiber plugs returned similar [9] or higher survival rates [21] for D7 blastocysts than D8 blastocysts. However, either lower [9] or higher [15,21] hatching rates were observed at 24 h post-warming when D7 or D8 expanded blastocysts were vitrified compared to our results. Further, the one-step warming of vitrified bovine D7 expanded blastocysts led to higher hatching rates assessed at 48 [15] or 72 h post-warming rather than 24 h post-warming [12,20].…”

Section: Discussioncontrasting

confidence: 87%

“…However, either lower [9] or higher [15,21] hatching rates were observed at 24 h post-warming when D7 or D8 expanded blastocysts were vitrified compared to our results. Further, the one-step warming of vitrified bovine D7 expanded blastocysts led to higher hatching rates assessed at 48 [15] or 72 h post-warming rather than 24 h post-warming [12,20].…”

Section: Discussioncontrasting

confidence: 87%

“…When using vitrification technology in veterinary practice, a practical approach is needed for the warming of vitrified embryos so that embryos can be directly and easily transferred to the uterus. Thus far, there have been several attempts to replace successive dilution steps with one-step in-straw cryoprotectant dilution [3][4][5][6][7][8][9][10][11][12][13][14][15] However, in some of these procedures, in-straw embryo warming requires more than one dilution step and the proper handling of the carrier system, thus demanding more accuracy when these techniques are to be used in the field by embryo-transfer practitioners [7,9,10,12]. Using the VitTrans device designed by our group, IVP embryos are easily warmed/diluted in-straw for their transfer to recipient females in field conditions [16].…”

Section: Introductionmentioning

confidence: 99%

“…The first step is usually an equilibration stage in a solution containing a relatively low CPA concentration, followed by ultra-short (30-90 s) exposure to a vitrification medium with a higher concentration of cryoprotectant (usually double the initial concentration) and dehydrating agent (usually a disaccharide, such as sucrose). Exposure to the equilibration medium may be short (e.g., 1 min followed by 25 s during vitrification) [12,19], last for 3min followed by vitrification for 25-30 s [9,15,20,21], or be even longer (e.g., 10-15 min followed by vitrification for 60 s) [22]. These durations have provided adequate blastocyst survival, blastocyst hatching, and pregnancy rates.…”

Section: Introductionmentioning

confidence: 99%

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A Shorter Equilibration Period Improves Post-Warming Outcomes after Vitrification and in Straw Dilution of In Vitro-Produced Bovine Embryos

Martínez-Rodero

García-Martínez

Ordóñez-León

et al. 2021

Biology

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This study was designed to the optimize vitrification and in-straw warming protocol of in vitro-produced bovine embryos by comparing two different equilibration periods, short equilibrium (SE: 3 min) and long equilibrium (LE: 12 min). Outcomes recorded in vitrified day seven (D7) and day eight (D8) expanded blastocysts were survival and hatching rates, cell counts, apoptosis rate, and gene expression. While survival rates at 3 and 24 h post-warming were reduced (p < 0.05) after vitrification, the hatching rates of D7 embryos vitrified after SE were similar to the rates recorded in fresh non-vitrified blastocysts. The hatching rates of vitrified D8 blastocysts were lower (p < 0.05) than of fresh controls regardless of treatment. Total cell count, and inner cell mass and trophectoderm cell counts were similar in hatched D7 blastocysts vitrified after SE and fresh blastocysts, while vitrified D8 blastocysts yielded lower values regardless of treatment. The apoptosis rate was significantly higher in both treatment groups compared to fresh controls, although rates were lower for SE than LE. No differences emerged in BAX, AQP3, CX43, and IFNτ gene expression between the treatments, whereas a significantly greater abundance of BCL2L1 and SOD1 transcripts was observed in blastocysts vitrified after SE. A shorter equilibration vitrification protocol was found to improve post-warming outcomes and time efficiency after in-straw warming/dilution.

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Section: Discussioncontrasting

confidence: 87%

Section: Discussioncontrasting

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

A Shorter Equilibration Period Improves Post-Warming Outcomes after Vitrification and in Straw Dilution of In Vitro-Produced Bovine Embryos

Martínez-Rodero

García-Martínez

Ordóñez-León

et al. 2021

Biology

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“…The formation of intracellular ice does not necessarily lead to fatal cell damage. However, if the rewarming rate is too slow during the rewarming process, recrystallization of intracellular ice can occur, which is fatal to cells (Liebermann & Tucker, 2004;Oliveira et al, 2020).…”

Section: Rewarmingmentioning

confidence: 99%

Clinical guideline for vascularized composite tissue cryopreservation

Yuan

et al. 2021

J Tissue Eng Regen Med

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At the Summit on Organ Banking through Converging Technologies held recently in Boston, tissue and organ cryopreservation technology was a topic of considerable interest. Although cryopreservation has been widely used in clinical practice, it currently remains limited to bloodless tissues with simple structures and functions that are small or thin, for example, ultra-thin skin, ovarian tissue slices, and other similar tissues. For whole organs, except for successful cryopreservation of rat ovaries ( 2002) and hind limbs (August 2002), successful cryopreservation of vascularized animal tissues or organs and their replantation have not yet been reported. We conducted histological and electron microscopic examinations on muscle after blood supply restoration to explain this problem and describe our experience with the goal of informing our colleagues to further develop the technology. To achieve broad application of vascularized tissue and organ cryopreservation, we have summarized our experience and established a clinical application scope for vascularized composite tissue cryopreservation.

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