In Utero Exposure to Maternal Electronic Nicotine Delivery System use Demonstrate Alterations to Craniofacial Development

Abstract

Objective Develop a model for the study of Electronic Nicotine Device (ENDS) exposure on craniofacial development. Design Experimental preclinical design followed as pregnant murine dams were randomized and exposed to filtered air exposure, carrier exposure consisting of 50% volume of propylene glycol and vegetable glycine (ENDS Carrier) respectively, or carrier exposure with 20 mg/ml of nicotine added to the liquid vaporizer (ENDS carrier with nicotine). Setting Preclinical murine model exposure using the SciReq exposure system. Participants C57BL6 adult 8 week old female pregnant mice and exposed in utero litters. Interventions Exposure to control filtered air, ENDS carrier or ENDS carrier with nicotine added throughout gestation at 1 puff/minute, 4 h/day, five days a week. Main Outcome Measures Cephalometric measures of post-natal day 15 pups born as exposed litters. Results Data suggests alterations to several facial morphology parameters in the developing offspring, suggesting electronic nicotine device systems may alter facial growth if used during pregnancy. Conclusions Future research should concentrate on varied formulations and exposure regimens of ENDS to determine timing windows of exposures and ENDS formulations that may be harmful to craniofacial development.

Manzamine-A Alters In Vitro Calvarial Osteoclast Function

“…Our group has a keen interest in the mechanism by which established and emerging drug exposures might alter health of the mineralized tissues of the body, specifically bone. − Understanding these interactions is critical as bone loss diseases are on the rise, notably osteoporosis. , The Center for Disease Control (United States) reports the age-adjusted prevalence of osteoporosis has increased in the past decade from 9.5% in 2008 to 12.6% in 2018 . Our most recent work has focused on MA and how the cells responsible for bone development, maintenance, and remodeling, osteoblasts, are affected by its exposure .…”