In Vitro 15Nitrogen-tracer Technique for Some Kinetic Measures of Ruminal Ammonia

Al-Rabbat, M. F.; Baldwin, R.L.; Weir, W. C.

doi:10.3168/jds.s0022-0302(71)85993-3

Cited by 46 publications

(26 citation statements)

References 30 publications

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“…The supernatant was used for determination of NH 3 ‐N concentration using (MgO) distillation method (Al‐Rabbat et al . ) and tVFA were estimated using steam distillation as described by Warner ().…”

Section: Methodsmentioning

confidence: 99%

“…Fifty milliliters of rumen liquor were deproteinized using 0.03 mol/mL of sulphuric acid (50 mL) and the volume was diluted with water to 500 mL in a volumetric flask and filtered. The supernatant was used for determination of NH3-N concentration using (MgO) distillation method (Al-Rabbat et al 1971) and tVFA were estimated using steam distillation as described by Warner (1964).…”

Section: Ruminal Fermentation Activitiesmentioning

confidence: 99%

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Effect of Mediterranean saltbush (Atriplex halimus) ensilaging with two developed enzyme cocktails on feed intake, nutrient digestibility and ruminal fermentation in sheep

Alsersy

Salem

Borhami

et al. 2014

Animal Science Journal

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The aim of this study was to assess the effects of feeding Atriplex halimus (AH) silage treated with two developed enzyme cocktails to sheep on feed intake, nutrient digestibility and ruminal fermentation. The AH silage was treated without or with 2 L of ZAD1(®) or ZAD2(®) /1000 kg with 5% molasses and ensiled for 30 days. Barley grain (300 g/head/day) was fed as an energy supplement once daily at 10.00 hours and AH silage with or without enzyme treatment was offered ad libitum to animals twice daily at 09.00 and 16.00 hours. Sheep were fed on four experimental forage diets comprised of AH silage and barley (D1), AH silage treated with ZAD1(®) and barley (D2), AH silage treated with ZAD2(®) and barley (D3) and AH silage treated with a combination of ZAD1(®) and ZAD2(®) (1:1) and barley (D4). Ensiling AH with enzymes reduced its contents of neutral detergent fiber and acid detergent fiber. The dry matter intake of AH of D2, D3 and D4 decreased (P < 0.001) as compared to D1. However, enzyme-treated diets had greater total digestible nutrients intake (P < 0.001) as compared to D1. The nutrients digestibility for D2, D3 and D4 were higher than those for D1 (P < 0.001), and were higher for D3 as compared to both D2 and D4. Sheep fed on D3 had highest (P < 0.001) ruminal total volatile fatty acids concentration, ammonia nitrogen concentration and microbial protein yield. It could be concluded that AH silage treated with ZAD1(®) or ZAD2(®) improved digestibility and rumen fermentation in sheep.

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Section: Methodsmentioning

confidence: 99%

Section: Ruminal Fermentation Activitiesmentioning

confidence: 99%

Effect of Mediterranean saltbush (Atriplex halimus) ensilaging with two developed enzyme cocktails on feed intake, nutrient digestibility and ruminal fermentation in sheep

Alsersy

Salem

Borhami

et al. 2014

Animal Science Journal

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“…Ruminal pH value measured using digital pH meter (Orian 680). Ammonia-N was carried out using MgO distillation method [28], while total VFA's were determined by steam distillation as described by Warner [29]. Total bacteria count was carried out according to Difco [30]; microbial nitrogen synthesis in the rumen according to the method of Makkar et al [31] using tungstic acid.…”

Section: Rumen Fermentation and In Situ Trialsmentioning

confidence: 99%

Effect of Magnetic Water on the Performance of Lactating Goats

MH¹

2015

JDVAR

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SummaryThis work was aimed to test the responses of zaraibi goats consumed magnetic water (field of ≈1200 and 3600 gauss) on rumen fermentation, milk and labneh yield and their components. Blood picture and antioxidant status were also studied. Fifteen lactating Zaraibi does (post weaning) were randomly divided into three equal groups (5 does each). First group, were drank tap water and regarded as control group, second and third groups were drank magnetic treated water with 1200 and 3600 gauss, respectively. While nine zaraibi bucks were used in three digestion trials. For rumen fermentation trials three does fitted with permanent rumen fistula were used. All groups of goats were provided free access to the water and fed the same ration [1]. Results indicated that:i. There was an improvement in water quality when exposed to the magnetic field;ii. Magnetic water significantly increased feeding values, nitrogen utilization, VFA's concentration, ruminal bacteria numbers and their activities and microbial protein (MP) syntheses, the highest values were recorded at the used level of 3600 gauss;iii. pH value, ammonia-N concentration and methane production was decreased with magnetic water;iv. DM and NDF with magnetic water were more effective degradability, while no significant different in CP degradability among groups;v. Third group (T2) had significantly increased feed intake and water consumption; it had significantly yielded more milk, 4% FCM, milk fat and protein than other groups;vi. Chemical composition and organoleptic properties of labneh were increased with magnetic groups;vii. some hematological and biochemical (glucose, total protein, albumin and globulin) parameters were significantly increased with magnetized water, while cholesterol and urea-N concentrations, AST and ALT were significantly decreased and viii. Magnetic water resulted in higher antioxidant compared to that drank unmagnetic water.In conclusion, magnetic treatment could improve water quality and consumption, feeding value, ruminal fermentation, blood picture, antioxidant status and hence milk and labneh yields especially with 3600 gauss.

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“…Most ruminal microbes can grow in defined media containing ammonia as sole source of nitrogen (BRYANT and ROBBINSON 1962), but there is increasing evidence that the mixed bacterial population uses preformed amino acids (AA) o r peptides for proliferation (AL-RABBAT et al 1971;NOLAN and LENG 1972;MARSDEN et al 1988) and that AA supplements improve microbial yields of various rumen bacterial species (COTTA and RUSSELL 1982) and of mixed population in a great extent (TAKAHASHI et al 1974a, b;ARGYLE and BALDWIN 1989;FUJIMAKI et al 1989). When 10 to 1000/0 of urea-nitrogen in 1000/0 urea media were replaced by an 18 AA mixture, microbial net protein synthesis of strained sheep rumen fluid was significantly higher in AA substituted media than in 100% urea media: the medium containing 75% urea-nitrogen plus 25% AA-nitrogen was the most effective in these incubations (TAKAHASHI et al 1974a).…”

Section: Introductionmentioning

confidence: 99%

Amino acid supplements: a least combination that increases microbial yields of washed cell suspension from goat rumen

Fujimaki¹,

Kobayashi²,

Wakita³

et al. 1992

Animal Physiology Nutrition

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Zusammenfassung Der Einfluß von Aminosäuren‐Supplementen auf die mikrobielle Synthese von gewaschenen Pansenzellsuspensionen von Ziegen Gewaschene Pansenzellsuspensionen von Ziegen wurden in Medien mit 75% Harnstoff‐N und 25% Aminosäuren‐N im Vergleich zu einem isonitrogenen und isoenergetischen Medium mit 100% Harnstoff‐N inkubiert, um das mikrobielle Wachstum zu ermitteln. Der größte Effekt mit höchster mikrobieller Synthese wurde bei der Anwendung von 18 Aminosäuren (18AA) erzielt, gefolgt von einer Inkubation mit 10 essentiellen Aminosäuren (10EAA) und mit 8 nicht essentiellen (8NEAA). Medien mit 17 Aminosäuren (18AA minus 1AA) und 7 Aminosäuren (8NEAA minus 1AA) zeigten mit Ausnahme beim Fehlen von Cystin die gleichen Effekte wie 18 AA und 8 NEAA auf die mikrobielle Synthese. Die Eliminierung von Valin, Leucin, Methionin oder Histidin aus dem 10 EAA‐Medium führte zu einer verringerten mikrobiellen Synthese. Bei der Eliminierung von jeweils einer der anderen Aminosäuren konnte kein Effekt beobachtet werden. Die Inkubation mit Leucin, Methionin und Histidin zeigte das gleiche mikrobielle Wachstum wie das 10 EAA‐Medium.

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In Vitro 15Nitrogen-tracer Technique for Some Kinetic Measures of Ruminal Ammonia

Cited by 46 publications

References 30 publications

Effect of Mediterranean saltbush (Atriplex halimus) ensilaging with two developed enzyme cocktails on feed intake, nutrient digestibility and ruminal fermentation in sheep

Effect of Mediterranean saltbush (Atriplex halimus) ensilaging with two developed enzyme cocktails on feed intake, nutrient digestibility and ruminal fermentation in sheep

Effect of Magnetic Water on the Performance of Lactating Goats

Amino acid supplements: a least combination that increases microbial yields of washed cell suspension from goat rumen

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