In Vitro 3D Modeling of Neurodegenerative Diseases

Louit, Aurélie; Galbraith, Todd; Berthod, François

doi:10.3390/bioengineering10010093

Cited by 11 publications

(9 citation statements)

References 168 publications

(272 reference statements)

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“…Neural tissue constructs that are created through bioprinting have the potential to serve as in vitro models for studying the pathophysiology of neurological disorders and for developing novel therapies [72]. Kajtez and colleagues introduced a novel method for engineering neuronal networks by using 3D printing technology to pattern human stem cells within self-healing annealable particle-extracellular matrix (SHAPE) composites (figure 5(a)).…”

Section: Disease Modelingmentioning

confidence: 99%

Recent advances and future directions of 3D to 6D printing in brain cancer treatment and neural tissue engineering

et al. 2023

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The field of neural tissue engineering has undergone a revolution due to advancements in 3D printing technology. This technology now enables the creation of intricate neural tissue constructs with precise geometries, topologies, and mechanical properties. Currently, there are various 3D printing techniques available, such as stereolithography and digital light processing, and a wide range of materials can be utilized, including hydrogels, biopolymers, and synthetic materials. Furthermore, the development of 4D printing has gained traction, allowing for the fabrication of structures that can change shape over time using techniques such as shape-memory polymers. These innovations have the potential to facilitate neural regeneration, drug screening, disease modeling, and hold tremendous promise for personalized diagnostics, precise therapeutic strategies against brain cancers. This review paper provides a comprehensive overview of the current state-of-the-art techniques and materials for 3D printing in neural tissue engineering and brain cancer. It focuses on the exciting possibilities that lie ahead, including the emerging field of 4D printing. Additionally, the paper discusses the potential applications of 5D and 6D printing, which integrate time and biological functions into the printing process, in the fields of neuroscience.

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Section: Disease Modelingmentioning

confidence: 99%

Recent advances and future directions of 3D to 6D printing in brain cancer treatment and neural tissue engineering

et al. 2023

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“…However, they have limited self‐renewal and differentiation capability as they do not include stem or progenitor cells. [ 23 ] An organoid can be considered as an advanced model of spheroid with geometries and features closer to the in vivo organ functional units. They spontaneously develop from stem cells, organ‐specific progenitors, and even tumor cells through a self‐organization process.…”

Section: Introductionmentioning

confidence: 99%

The Evolution of Technology‐Driven In Vitro Models for Neurodegenerative Diseases

De Vitis,

Stanzione,

Romano

et al. 2024

Advanced Science

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The alteration in the neural circuits of both central and peripheral nervous systems is closely related to the onset of neurodegenerative disorders (NDDs). Despite significant research efforts, the knowledge regarding NDD pathological processes, and the development of efficacious drugs are still limited due to the inability to access and reproduce the components of the nervous system and its intricate microenvironment. 2D culture systems are too simplistic to accurately represent the more complex and dynamic situation of cells in vivo and have therefore been surpassed by 3D systems. However, both models suffer from various limitations that can be overcome by employing two innovative technologies: organ‐on‐chip and 3D printing. In this review, an overview of the advantages and shortcomings of both microfluidic platforms and extracellular matrix‐like biomaterials will be given. Then, the combination of microfluidics and hydrogels as a new synergistic approach to study neural disorders by analyzing the latest advances in 3D brain‐on‐chip for neurodegenerative research will be explored.

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“…A cerebellum organoid model has also been developed to recapitulate the complexity of cerebellar tissue [17]. However, organoids sometimes cause some reproducibility issues [18][19][20].…”

Section: Introductionmentioning

confidence: 99%

In Vitro Characterization of Motor Neurons and Purkinje Cells Differentiated from Induced Pluripotent Stem Cells Generated from Patients with Autosomal Recessive Spastic Ataxia of Charlevoix-Saguenay

Louit

Beaudet

Blais

et al. 2023

Stem Cells International

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Autosomal recessive spastic ataxia of Charlevoix-Saguenay (ARSACS) is an early-onset neurodegenerative disease mainly characterized by spasticity in the lower limbs and poor muscle control. The disease is caused by mutations in the SACS gene leading in most cases to a loss of function of the sacsin protein, which is highly expressed in motor neurons and Purkinje cells. To investigate the impact of the mutated sacsin protein in these cells in vitro, induced pluripotent stem cell- (iPSC-) derived motor neurons and iPSC-derived Purkinje cells were generated from three ARSACS patients. Both types of iPSC-derived neurons expressed the characteristic neuronal markers β3-tubulin, neurofilaments M and H, as well as specific markers like Islet-1 for motor neurons, and parvalbumin or calbindin for Purkinje cells. Compared to controls, iPSC-derived mutated SACS neurons expressed lower amounts of sacsin. In addition, characteristic neurofilament aggregates were detected along the neurites of both iPSC-derived neurons. These results indicate that it is possible to recapitulate in vitro, at least in part, the ARSACS pathological signature in vitro using patient-derived motor neurons and Purkinje cells differentiated from iPSCs. Such an in vitro personalized model of the disease could be useful for the screening of new drugs for the treatment of ARSACS.

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In Vitro 3D Modeling of Neurodegenerative Diseases

Cited by 11 publications

References 168 publications

Recent advances and future directions of 3D to 6D printing in brain cancer treatment and neural tissue engineering

Recent advances and future directions of 3D to 6D printing in brain cancer treatment and neural tissue engineering

The Evolution of Technology‐Driven In Vitro Models for Neurodegenerative Diseases

In Vitro Characterization of Motor Neurons and Purkinje Cells Differentiated from Induced Pluripotent Stem Cells Generated from Patients with Autosomal Recessive Spastic Ataxia of Charlevoix-Saguenay

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