In vitro action of 2.2’-azobis-2 amidinopropan dihydrochloride,red wine polyphenols, resveratrol and catechin on anion permeability for chloride in human red blood cell

Gallo, Gaetano; Martino, G.

doi:10.5530/fra.2014.2.3

Cited by 3 publications

(3 citation statements)

References 14 publications

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“…3 In a recent paper 5 authors demonstrated that hypertriglyceridemia affects Red Blood Cell membrane oxidative status (increase of [MDA] and [HNE] to membrane protein ratio) is increased by high levels of blood cholesterol, but selectively by hypertriglyceridemia. 5,6 It is known that Cr protects the erythrocytes by attenuating the AAPH and H 2 O 2 induced alterations. 6 This protective effect was confirmed by electron microscopic analysis which showed that oxidant-induced cell damage was attenuated by Cre.…”

Section: Introductionmentioning

confidence: 99%

Do Creatinine Blood Levels Act upon Fatty Acid Metabolites in Human Red Blood Cell Membranes?

Gallo

Martino

2020

FRA

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Objectives: The aim of the present research is to correlate creatininemia and uricemia to HNE and MDA levels of Red Blood Cell purified membranes in comparison to those of normal subjects to characterize the effects of oxidative stress. Methods: The studied population consisted of 10 patients with endogenous both hypercreatininemic hyperuricemic and ten normal subjects. The measurement of total proteins in RBC ghosts and HNE and MDA are conducted on blood samples of patients. Results: The obtained data evidence that both creatinine and uric acid levels have influence on the ratio of both MDA/protein and HNE/protein content on RBC ghosts, demonstrating their possible protective role against oxidative stress at low concentrations in blood and oxidizing power at higher concentrations. Conclusion: The displayed results in this study suggest that at low concentrations of creatinine and uric acid there is oxidative stress in human erythrocyte membrane, but if creatinine and uric acid have slowed down to sufficient limits there is also oxidative stress, as demonstrated by MDA and HNE levels; only if the concentration of these two substances overwhelms a threshold. .) Studies on the mitochondrial metabolism by either environmental or endogenous regulating factors, the regulation of interstitial compartment, bone and the bonelike biomaterials structure and properties. From 1992 he refined fluorescence microscopy and image analysis methods to evaluate ion transport, NO metabolism and oxidative stress of cells in culture . From 2006 he studies also oxidative stress and enzyme scavenging activities in experimental animals and human subjects. He has 60 international publications with referees.

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Section: Introductionmentioning

confidence: 99%

Do Creatinine Blood Levels Act upon Fatty Acid Metabolites in Human Red Blood Cell Membranes?

Gallo

Martino

2020

FRA

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“…4-hydroxytyrosol shows a substantial degree of inhibition against ascorbate-Fe 2+ induced LPO in rat liver microsomes. [ 6 ] In a previous study of Gallo and Martino[ 7 ] on RBC membranes damages by oxidant agents, authors demonstrated the conformational alterations and polyphenols repairing in the same structure. Among the known risk factors of atherosclerosis-associated oxidative stress are hyperlipidemia and/or hyperglycemia.…”

Section: Introductionmentioning

confidence: 99%

“…[ 21 ] 4-hydroxytyrosol is shown to significantly protect RBCs from oxidative damage (LPO [4-HNE]). The authors[ 7 ] studied the protective effect of 4-hydroxytyrosol on human RBCs and oxidative damage so, the intent is to examine the role of triglycerides overload on degradation of membrane lipids in the presence of other reducing agents (protective effect of 4-hydroxytyrosol). For instance, in complete eukaryotic cells, the following damages can happen: 4-HAE are endogenous ligands for peroxisome proliferator-activated receptor-δ.…”

Section: Introductionmentioning

confidence: 99%

Are polyunsaturated fatty acid metabolites, the protective effect of 4-hydroxytyrosol on human red blood cell membranes and oxidative damage (4-hydroxyalkenals) compatible in hypertriglyceridemic patients?

Gallo

Bruno

Taranto³

et al. 2017

Phcog Mag

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Background:Increased levels of malondialdehyde (MDA) and 4-hydroxynonenal (HNE) are demonstrated in plasma of uremic patients. A study showed that the comparison of erythrocytes of healthy and diseased patients (obese, hypertensive, and Type 2 diabetics) with age is associated to a disturbed oxidant/antioxidant balance when obesity is associated with hypertension. 4-hydroxytyrosol is shown to significantly protect red blood cells (RBCs) from oxidative damage (4-HNE). In literature, there are partial discussions on the role of lipids and their oxidation products. The products of degradation of membrane proteins are observed as self-consisting products without interrelations with membrane lipids.Objective:The aim of this study is to evaluate the role of polyunsaturated fatty acid (PUFA) metabolites on oxidative damage (4-hydroxy-alkenals) in RBCs of hypertriglyceridemic patients after membrane treatment with 4-hydroxytyrosol.Materials and Methods:The authors optimize the isolation of RBC ghosts and spectrophotometric method to measure free 4-hydroxyalkenals in human RBC membranes and investigated the effect on oxidative damage in human erythrocyte membranes and in vitro 4-hydroxytyrosol treatment to evaluate the membrane lipids reducible by this phenol.Results:Plasma triglyceride levels in patients are clearly higher than in controls. Moreover, total membrane proteins data are similar to previous described. The normalized alkenals levels are significantly enhanced in hyperlipemic patients in comparison to normoglyceridemic controls. After the 4-hydroxytyrosol action, lipid metabolites substantially decrease. The ratio of oxidized lipids (MDA + HNE) and membrane proteins data are similar to previously described ones.Conclusion:According to experimental data, the accumulation of the alkenals in RBC membrane could be produced either by partial PUFA oxidation contained in glycerides and plasma glycerides and by glycerides into plasma membrane recycled RBC.SUMMARY Hypertriglyceridemia induces oxidative stress in human red blood cell (RBC) membranesOxidative stress causes increased plasma membrane total protein concentration and hydroxynonenal and malondialdehyde levelsThe authors optimize the isolation of RBC ghosts and spectrophotometric method to measure free 4-hydroxyalkenals in human RBC membranesAfter the reduction with 4-hydroxytyrosol, oxidized lipid concentration significantly decrease. Abbreviations used: RBC: Red blood cell; MDA: Malondialdehyde; HNE\HAE: 4-hydroxyalkenals; LPO: Lipid peroxidation; ROS: Reactive oxygen species; ORAC: Oxygen Radical Absorbance Capacity.

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Reducing Compounds Roles in Oxidative Stress Relieving of Human Red Blood Cells

Gallo¹

2022

Reactive Oxygen Species

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Oxidative stress is the consequence of an imbalance between pro-oxidant and antioxidant processes. Antioxidants that counteract reactive oxygen species do not all work the same way. Both resveratrol and the more powerful 4-hydroxytyrosol are excellent reducing agents. Polyphenol treatment (red wine polyphenols, resveratrol and catechin) is associated with a significant increase in anion permeability for chloride compared with control and 2.2′-azobis-2 amidinopropan dihydrochloride affected cells. Treatment with polyphenols was associated with a significant reduction in mean ± standard error of the mean membrane lipid peroxidation compared with control and 2.2′-azobis-2 amidinopropan dihydrochloride treatment. Hemolysis data are also obtained in the previously described conditions. 4-hydroxytyrosol is shown to significantly protect red blood cells from oxidative damage by 4-hydroxynonenal. But there are paradoxical effects like uric acid and creatinine. The obtained data evidence that both creatinine and uric acid levels have influence on the ratio of both malondialdehyde/protein and 4-hydroxynonenal/protein content on red blood cell ghosts, demonstrating their possible protective role against oxidative stress at low concentrations in blood and oxidizing power at higher concentrations. Finally, polyunsaturated fatty acids do not have all this reducing power.

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In vitro action of 2.2’-azobis-2 amidinopropan dihydrochloride,red wine polyphenols, resveratrol and catechin on anion permeability for chloride in human red blood cell

Cited by 3 publications

References 14 publications

Do Creatinine Blood Levels Act upon Fatty Acid Metabolites in Human Red Blood Cell Membranes?

Do Creatinine Blood Levels Act upon Fatty Acid Metabolites in Human Red Blood Cell Membranes?

Are polyunsaturated fatty acid metabolites, the protective effect of 4-hydroxytyrosol on human red blood cell membranes and oxidative damage (4-hydroxyalkenals) compatible in hypertriglyceridemic patients?

Reducing Compounds Roles in Oxidative Stress Relieving of Human Red Blood Cells

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