1991
DOI: 10.1128/aac.35.6.1232
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In vitro activities of aminoglycosides, lincosamides, and rifamycins against Mycobacterium leprae

Abstract: The in vitro activities of a variety of aminoglycosides, lincosamides, and rifamycins against Mycobacterium leprae were evaluated with the BACTEC 460 system. At 20 ,g/ml, gentamicin, kanamycin, tobramycin, streptomycin, and amikacin were inactive. Lincomycin was active at 20 ,ug/ml, and clindamycin was active at 0.31 ,ug/mi. Rifamycin SV, rifabutin, and rifampin were active at 3.1, 3.1 to 12.5, and 200 ng/ml, respectively. The M. leprae inoculum was prepared from infected nude mouse footpads and partially puri… Show more

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Cited by 7 publications
(4 citation statements)
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“…The present report is the first to use this method to study drug activity against intracellular M. leprae and to study the activity of a single drug both intracellularly and extracellularly. The roughly equal activity of fusidic acid against both intracellu- (6,7,11). Conversely, the Buddemeyer-type detection system is more sensitive (because of the amplification of the signal in the liquid scintillation counting system), and we often find it more useful when working with macrophage-resident (1,19) or biopsy-derived M. leprae.…”
Section: Discussionmentioning
confidence: 99%
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“…The present report is the first to use this method to study drug activity against intracellular M. leprae and to study the activity of a single drug both intracellularly and extracellularly. The roughly equal activity of fusidic acid against both intracellu- (6,7,11). Conversely, the Buddemeyer-type detection system is more sensitive (because of the amplification of the signal in the liquid scintillation counting system), and we often find it more useful when working with macrophage-resident (1,19) or biopsy-derived M. leprae.…”
Section: Discussionmentioning
confidence: 99%
“…We have previously demonstrated the utility of radiorespirometry in evaluating the in vitro activities of a variety of macrolides (9), fluoroquinolones (11), phenazines (10,12), and aminoglycosides, lincosamides, and rifamycins (7) against M. leprae in axenic media with either a Buddemeyertype detection system (5) or the BACTEC 460 instrument (6) as a means of assessing drug activity in the absence of bacterial multiplication. We and our colleagues have also used assays quantitating phenolic glycolipid-1 synthesis and adenosine triphosphate concentration (8) as indices of drug activity against both extracellular (16) and intracellular (20) M. leprae.…”
Section: Discussionmentioning
confidence: 99%
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“…Rifampicin, a potent inhibitor of DNAdependent RNA polymerase of bacteria, is used extensively as a prokaryotic transcriptional inhibitor. Together with its semisynthetic derivatives, rifampitin is used against mycobacterium infections [5,16], staphylococcus infections [S] and a wide variety of other pathogens. The mechanism of its action is based on the binding of rifampicin to the p subunit of bacterial RNA polymerase which interferes with the formation of the first phosphodiester bond in the Xpl2 infection.…”
Section: Introductionmentioning
confidence: 99%