In vitro activity of 2-phenylethyl glucosinolate, and its hydrolysis derivatives on the root-knot nematode Globodera rostochiensis (Woll.)

Serra, B.; Rosa, Eduardo; Iori, Renato; Barillari, Jessica; Cardoso, Alexandre Moraes; Abreu, Cláudia; Rollin, Patrick

doi:10.1016/s0304-4238(01)00277-1

Cited by 31 publications

(21 citation statements)

References 8 publications

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“…The biological effects of GSLs and GHPs have been known since the early 1990s, when several authors investigated their effects on the growth and development of bacteria (19,23), insects (24)(25)(26), fungi (27,28), and nematodes (29,30), and our knowledge about the deterrent or attractant effects of the main glucosinolates on different pests (generalists and specialists) and parasitoids is well documented. Other authors have tested the effects of GHPs and GSLs on soil pathogens by incorporating Brassica residues into soil or by testing their effect by using in vitro assays.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Activity of Glucosinolates and Their Degradation Products against Brassica-Pathogenic Bacteria and Fungi

Sotelo

Márquez

Soengas

et al. 2015

Appl Environ Microbiol

112

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bGlucosinolates (GSLs) are secondary metabolites found in Brassica vegetables that confer on them resistance against pests and diseases. Both GSLs and glucosinolate hydrolysis products (GHPs) have shown positive effects in reducing soil pathogens. Information about their in vitro biocide effects is scarce, but previous studies have shown sinigrin GSLs and their associated allyl isothiocyanate (AITC) to be soil biocides. The objective of this work was to evaluate the biocide effects of 17 GSLs and GHPs and of leaf methanolic extracts of different GSL-enriched Brassica crops on suppressing in vitro growth of two bacterial (Xanthomonas campestris pv. campestris and Pseudomonas syringae pv. maculicola) and two fungal (Alternaria brassicae and Sclerotinia scletoriorum) Brassica pathogens. GSLs, GHPs, and methanolic leaf extracts inhibited the development of the pathogens tested compared to the control, and the effect was dose dependent. Furthermore, the biocide effects of the different compounds studied were dependent on the species and race of the pathogen. These results indicate that GSLs and their GHPs, as well as extracts of different Brassica species, have potential to inhibit pathogen growth and offer new opportunities to study the use of Brassica crops in biofumigation for the control of multiple diseases.T he genus Brassica belongs to the family Brassicaceae (also known as Cruciferae); economically speaking, it is the most important genus within the tribe Brassicaceae, containing 37 different species. Brassica vegetables are of great economic importance throughout the world. Currently, Brassica crops, together with cereals, represent the basis of world food supplies. In 2007, Brassica vegetables were cultivated in more than 142 countries around the world, and they occupied more than 4.1 million ha (1).The productivity and quality of important Brassica crops (e.g., cabbage, oilseed rape, cauliflower, Brussels sprouts, kale, and broccoli) are seriously affected by several diseases, which result in substantial economic losses (2). Black rot, caused by the bacterium Xanthomonas campestris pv. campestris (Pammel), is considered to be one of the most important pathogens affecting Brassica vegetables worldwide (3). There are nine races of Xanthomonas campestris pv. campestris: races 1 to 6 were described by Vicente et al. (4) and races 7 to 9 by Fargier and Manceau (5). It is recognized that races 1 and 4 are the most virulent and widespread, accounting for most of the black rot recorded around the world (4).Bacterial leaf spot, caused by Pseudomonas syringae pv. maculicola (McCulloch) (6), is very significant on cauliflower but also occurs on broccoli, Brussels sprouts, and other brassicas. P. syringae pv. maculicola may also cause leaf blight on the oilseed species Brassica juncea and Brassica rapa (3).Sclerotinia stem rot, caused by Sclerotinia sclerotiorum (Lib.) de Bary, is a widespread fungal disease in temperate areas of the world and also occurs in warmer and drier areas during the winter months or the rain...

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Section: Discussionmentioning

confidence: 99%

In Vitro Activity of Glucosinolates and Their Degradation Products against Brassica-Pathogenic Bacteria and Fungi

Sotelo

Márquez

Soengas

et al. 2015

Appl Environ Microbiol

112

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“…This is thought to be associated with the conversion of the indolyl GSL to IAA and its role in the gall formation associated with the disease. Lab studies convincingly show that various GSL hydrolysis products are toxic to plant parasitic nematodes (Lazzeri et al 1993;Serra et al 2002). There is some support for these studies from field plants as well.…”

Section: Evidence For Gsl As Mediators For Belowground Organisms In Pmentioning

confidence: 90%

“…Lazzeri et al 1993;Serra et al 2002), there are few clear examples of resistance mechanisms in Brassicacea related to root GSL in planta. Although there are far more studies on the roles of GSL between shoots and aboveground organisms, there are some lines of evidence root GSL may similarly affect both specialist and generalist soil-dwelling pathogens, nematodes and insects.…”

Section: Evidence For Gsl As Mediators For Belowground Organisms In Pmentioning

confidence: 99%

Root and shoot glucosinolates: a comparison of their diversity, function and interactions in natural and managed ecosystems

2008

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The role of glucosinolates in aboveground plant-insect and plant-pathogen interactions has been studied widely in both natural and managed ecosystems. Fewer studies have considered interactions between root glucosinolates and soil organisms. Similarly, data comparing local and systemic changes in glucosinolate levels after root-and shoot-induction are scarce. An analysis of 74 studies on constitutive root and shoot glucosinolates of 29 plant species showed that overall, roots have higher concentrations and a greater diversity of glucosinolates than shoots. Roots have significantly higher levels of the aromatic 2-phenylethyl glucosinolate, possibly related to the greater effectiveness and toxicity of its hydrolysis products in soil. In shoots, the most dominant indole glucosinolate is indol-3-ylglucosinolate, whereas roots are dominated by its methoxyderivatives. Indole glucosinolates were the most responsive after jasmonate or salicylate induction, but increases after jasmonate induction were most pronounced in the shoot. In general, root glucosinolate levels did not change as strongly as shoot levels. We postulate that roots may rely more on high constitutive levels of glucosinolates, due to the higher and constant pathogen pressure in soil communities. The differences in root and shoot glucosinolate patterns are further discussed in relation to the molecular regulation of glucosinolate biosynthesis, the within-tissue distribution of glucosinolates in the roots, and the use of glucosinolate-containing crops for biofumigation. Comparative studies of tissue-specific biosynthesis and regulation in relation to the biological interactions in aboveground and belowground environments are needed to advance investigations of the evolution and further utilization of glucosinolates in natural and managed ecosystems.

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“…However, the myrosinase-generated hydrolysis products specifically ITC, also recognised as major inhibitors of microbial activity, have been shown to effectively control nematodes (Lazerri et al 1993Kirkegaard & Sarwar 1998Serra et al 2002;Buskov et al 2002). The effectiveness of the ITC is depended on its structure, with 2-phenylethyl-isothiocyanate being the least effective with LD50 (0.05 mg.ml −1 ) on nematodes .…”

Section: Introductionmentioning

confidence: 99%

Suppressing Potato Cyst Nematode, Globodera rostochiensis, with Extracts of Brassicacea Plants

et al. 2009

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The golden potato cyst nematode, Globodera rostochiensis (Woll) is responsible for large yield losses in potato crops in northern Portugal. Fumigation with synthetic chemicals controls this soil pest with possible negative impact on the environment. Thus, it is desirable to find environmentally friendly alternative measures to control this nematode species. Amending plant parts of Brassicaceae have been recognized as potential biofumigation practices. Active volatiles compounds released after enzymatic hydrolysis are responsible for the efficacy of biofumigants. The glucosinolate-myrosinase-isothiocyanates system appears to be controlling several soilborne pests and pathogens. The biological role of Brassicacea green manure amendments on potato cyst nematodes populations has not been reported in Portugal. In this study we evaluated the effects of two concentrations (0.2 and 0.05µmoles 100 g −1 dry weight) of total glucosinolates of six plants extracts (broccoli, cauliflower, collards of Brassica rapa, collards of kale, Portuguese cabbage and watercress) on potato cyst nematodes population. The results were expressed as number of new cysts formed on bioassay Desiree potato roots. ANOVA analysis showed a highly significant effect (P < 0.001) of the plant extracts and concentrations tested on the suppression of potato cysts nematodes. The LSD test showed that the number of newly formed cyst on root system of bioassay plant was also related (P<0.05) to type and concentrations of glucosinolates extracts. The lowest numbers of cysts were recovered from potato roots that received extracts of watercress, cauliflower and B. Rapa, respectively. The cyst reduction appeared to be associated with high levels of 2-Phenthyl and 2-propenyl glucosinolates in these plants.Resumen El nematodo dorado del quiste de la papa, Globodera rostochiensis (Woll) es responsable de graves pérdidas de rendimiento en cultivos de papa en el norte de Portugal. La fumigación con químicos sintéticos controla esta plaga del suelo con posible impacto negativo en el medio ambiente. Por lo tanto es deseable encontrar alternativas que sean amigables con el medio ambiente para controlar este nematodo. Enmiendas del suelo a partir de partes de plantas de crucíferas han sido reconocidas como practicas potenciales de bio fumigación. Compuestos volátiles activos liberados después de la hidrólisis enzimática son los responsables de la eficacia de biofumigantes. El sistema glucosinolato-mirosinasa-isotiocianato parece ser el control de varias plagas plagas y patógenos del suelo. El rol biológico de las enmiendas del suelo de abono verde de Brassicacea en las poblaciones de nematodos del quiste de la papa no ha sido reportado en Portugal. En este estudio evaluamos los efectos de 2 concentraciones (0.2 y 0.05 umoles 100g −1 peso seco) de glucosinolatos totales de seis extractos de plantas (brócoli, coliflor, hojas de nabo, hojas de kale, col Portuguesa y berro) en la población del quiste del nematodo de la papa. Los resultados se expresaron como número de quistes...

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In vitro activity of 2-phenylethyl glucosinolate, and its hydrolysis derivatives on the root-knot nematode Globodera rostochiensis (Woll.)

Cited by 31 publications

References 8 publications

In Vitro Activity of Glucosinolates and Their Degradation Products against Brassica-Pathogenic Bacteria and Fungi

In Vitro Activity of Glucosinolates and Their Degradation Products against Brassica-Pathogenic Bacteria and Fungi

Root and shoot glucosinolates: a comparison of their diversity, function and interactions in natural and managed ecosystems

Suppressing Potato Cyst Nematode, Globodera rostochiensis, with Extracts of Brassicacea Plants

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