In vitro activity of a novel compound, Mul-1867, against clinically significant fungi Candida spp. and Aspergillus spp.

Tetz, George; Cynamon, Michael H.; Hendricks, Gregory M.; Vikina, Daria; Tetz, Victor

doi:10.1016/j.ijantimicag.2017.02.011

Cited by 7 publications

(13 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The leakage of cytoplasmic components is an indicator of disintegrity of the cell membrane. Because nucleic acid macromolecules have strong UV absorption at 260 nm, they can be used as a marker of cell leakage. , The measurement of the absorbance at 260 nm has been used as evidence to determine the leakage of nucleic acid molecules, which is an indication of the disruption of fungal cell membranes. , C. albicans were treated with α-PL3-G100 at final concentrations of 4× MIC, 2× MIC, and 1× MIC for 4 h, respectively (Figure c).…”

Section: Resultsmentioning

confidence: 99%

“…60,61 The measurement of the absorbance at 260 nm has been used as evidence to determine the leakage of nucleic acid molecules, which is an indication of the disruption of fungal cell membranes. 62,63 C. albicans were treated with α-PL3-G100 at final concentrations of 4× MIC, 2× MIC, and 1× MIC for 4 h, respectively (Figure 6c). The OD 260 of the resulting supernatant was higher than that of the control and increased with the polymer concentration.…”

Section: Measurement Of Circular Dichroism (Cd)mentioning

confidence: 99%

See 1 more Smart Citation

Guanylation Significantly Enhances the Antifungal Activity of Poly(α-lysine)

Liu

Han

Liu

et al. 2022

ACS Appl. Polym. Mater.

View full text Add to dashboard Cite

AMP-mimicking polymers have the potential to serve as an alternative to small-molecule antifungal drugs. Herein, we synthesized four different molecular weight poly(α-lysine) (α-PLn, n = 1−4) and further modified α-PLn to afford guanylated α-PLn (α-PLn-Gx, where x denotes the percentage of guanylation) with enhanced antifungal activities. α-PLn exhibited good antibacterial activities against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus), with minimal inhibitory concentration (MIC) values of 24−96 μg/mL, but it did not show antifungal activities against Candida albicans, Candida parapsilosis, Candida krusei, and Candida glabrata, except for Candida tropicalis (MIC < 3 μg/mL). After guanylation, the antifungal activities of α-PLn-Gx against C. albicans, C. krusei, and C. parapsilosis were significantly improved when x ≥ 80 and the MIC values of α-PL3-G100 were reduced by >32-fold, 64-fold, and >16fold, respectively, compared to that of the corresponding α-PL3. The mechanistic studies demonstrated that α-PL3-G100 disrupted the integrity of cell membranes of C. albicans.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Measurement Of Circular Dichroism (Cd)mentioning

confidence: 99%

Guanylation Significantly Enhances the Antifungal Activity of Poly(α-lysine)

Liu

Han

Liu

et al. 2022

ACS Appl. Polym. Mater.

View full text Add to dashboard Cite

show abstract

“…Moreover, M451 also inhibited the germination of F. oxysporum spores. Previous studies have revealed that this class of compounds does not have significant cytotoxicity; therefore, the observed sporicidal effect could not be addressed due to corrosive effects (Tetz G et al, 2017). The high efficacy of M451 against strains of difficult-to-treat plant pathogens, along with its lack of cross-resistance and high sporicidal activity, means that this compound may be employed as an agricultural fungicide for the successful control of fungal diseases in plants.…”

Section: Discussionmentioning

confidence: 99%

“…Here we describe the activity of M451, which is a complex of the novel antimicrobial agent TGV-28 poly-N-carboxamido-1,6-diaminohexane) particularly N’-aminated and 0.01% NaH 2 PO 4 . Similar to its predecessor, Mul-1867, the mechanism of action of TGV-28 is a nonspecific attack on the cell walls of fungi (Tetz G., Tetz V., 2015; Tetz, G. et al, 2017). This study aimed to determine the in vitro activity of M451 against various phytopathogenic fungi.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Antifungal activity of the novel compound M451 against phytopathogens

Tetz¹,

Кардава²,

Krasnov³

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

Phytopathogenic fungi are the dominant causal agents of plant diseases. Currently available fungicides have significant disadvantages, being insufficiently effective owing to both intrinsic tolerance and the spread of antibiotic resistance accumulating in plant tissues, posing a global threat to public health. Finding a new broad-spectrum fungicide is a challenge for plant protection. We studied the potency of a novel antimicrobial agent, M451, against different phytopathogenic fungi of the phyla Ascomycota, Oomycota, and Basidiomycota. M451 exhibited significant antifungal activity with EC50 values ranging from 34 to 145 ug/mL. Analysis of the minimal fungicidal concentration and conidial destruction assay revealed that M451 possesses the highest activity compared with different polyene, azole, and phenylpyrrole antifungals against Fusarium oxysporum. Time-kill analysis revealed that M451 was the only antifungal agent tested that exhibited antifungal activity within 5 min of exposure. Spore production and germination were also significantly inhibited by M451 treatment. Based on the broad spectrum of antifungal effects across different plant pathogens, M451 could be a new chemical fungicide for plant disease management.

show abstract