In Vitro Activity of Amoxicillin, Clindamycin, Doxycycline, Metronidazole, and Moxifloxacin Against Oral Actinomyces

LeCorn, Demetrick W.; Vertucci, Frank J.; Rojas, Maria Fernanda; Progulske‐Fox, Ann; Bélanger, Myriam

doi:10.1016/j.joen.2007.02.002

Cited by 41 publications

(29 citation statements)

References 30 publications

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“…Nonetheless, the amount of drugs released from the TAP-mimic scaffold throughout the study is well above (Fig. 2A) the minimum inhibitory concentration for A. naeslundii (eg, antibiotic within the tetracycline family such as doxycycline, minimum inhibitory concentration = 0.064 μ g/mL) (5). …”

Section: Discussionmentioning

confidence: 92%

“…Previous studies have investigated A. naeslundii associated with other bacteria, aiming to form multispecies biofilms (5, 28). To the best of our knowledge, no attempt has been made to study the influence of time on the formation of A. naeslundii biofilm on dentin.…”

Section: Discussionmentioning

confidence: 99%

“…However, no study has investigated the antimicrobial efficacy of TAP on A. naeslundii dentin biofilm. In vitro research has demonstrated that Actinomyces species were resistant to MET but susceptible to moxifloxacin (ie, CIP family) and amoxicillin (5). Therefore, one could certainly argue that both CIP and MINO present in the TAP-mimic scaffold were responsible for most of the antimicrobial action against A. naeslundii biofilm.…”

Section: Discussionmentioning

confidence: 99%

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Antimicrobial Effects of Novel Triple Antibiotic Paste–Mimic Scaffolds on Actinomyces naeslundii Biofilm

Albuquerque

Ryan

Münchow

et al. 2015

Journal of Endodontics

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Introduction Actinomyces naeslundii has been recovered from traumatized permanent teeth diagnosed with necrotic pulps. In this work, a triple antibiotic paste (TAP)–mimic scaffold is proposed as a drug-delivery strategy to eliminate A. naeslundii dentin biofilm. Methods Metronidazole, ciprofloxacin, and minocycline were added to a polydioxanone (PDS) polymer solution and spun into fibrous scaffolds. Fiber morphology, mechanical properties, and drug release were investigated by using scanning electron microscopy, microtensile testing, and high-performance liquid chromatography, respectively. Human dentin specimens (4 × 4 × 1 mm3, n = 4/group) were inoculated with A. naeslundii (ATCC 43146) for 7 days for biofilm formation. The infected dentin specimens were exposed to TAP-mimic scaffolds, TAP solution (positive control), and pure PDS (drug-free scaffold). Dentin infected (7-day biofilm) specimens were used for comparison (negative control). Confocal laser scanning microscopy was done to determine bacterial viability. Results Scaffolds displayed a submicron mean fiber diameter (PDS = 689 ± 312 nm and TAP-mimic = 718 ± 125 nm). Overall, TAP-mimic scaffolds showed significantly (P ≤ .040) lower mechanical properties than PDS. Within the first 24 hours, a burst release for all drugs was seen. A sustained maintenance of metronidazole and ciprofloxacin was observed over 4 weeks, but not for minocycline. Confocal laser scanning microscopy demonstrated complete elimination of all viable bacteria exposed to the TAP solution. Meanwhile, TAP-mimic scaffolds led to a significant (P < .05) reduction in the percentage of viable bacteria compared with the negative control and PDS. Conclusions Our findings suggest that TAP-mimic scaffolds hold significant potential in the eradication/elimination of bacterial biofilm, a critical step in regenerative endodontics.

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Section: Discussionmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Antimicrobial Effects of Novel Triple Antibiotic Paste–Mimic Scaffolds on Actinomyces naeslundii Biofilm

Albuquerque

Ryan

Münchow

et al. 2015

Journal of Endodontics

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“…Furthermore, previous studies have shown antimicrobial effect of doxycycline against wide range of oral microbialflora. [19][20][21] It has also been claimed that the acidic pH of citric acid and its chelating property are effective in destroying bacterial isolates although the exact mechanism is not known.…”

Section: Discussionmentioning

confidence: 99%

Comparative antimicrobial efficacy of herbal alternatives ( Emblica officinalis , Psidium guajava ), MTAD, and 2.5% sodium hypochlorite against Enterococcus faecalis : An in vitro study

Dubey¹

2016

Journal of Oral Biology and Craniofacial Research

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“…De acordo com os resultados obtidos, levando-se em consideração os valores de concentração inibitória mínima (CIM) determinados pela NCCLS, todas as cepas de E. faecalis, E. faecium, A. viscosus e S. aureus mostraram-se 100% suscetíveis a Benzilpenicilina (PG), Amoxicilina (AC), Amoxicilina + ácido clavulânico (XL), Vancomicina (VA) e Moxifloxacina (MX), corroborando com os achados de outros estudos 9,17,19,25 . Este resultado confirma a utilização da penicilina e seus derivados como antibióticos de primeira escolha no tratamento de infecções de origem endodôntica, e a utilização na terapêutica profilática contra a endocardite bacteriana 16,24 .…”

Section: Discussionunclassified

Avaliação da suscetibilidade antimicrobiana de bactérias anaeróbias facultativas isoladas de canais radiculares de dentes com insucesso endodôntico frente aos antibióticos de uso sistêmico

Santi

Barbosa-Ribeiro

Endo

et al. 2015

Rev. odontol. UNESP

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ResumoIntrodução: Bactérias associadas ao insucesso do tratamento endodôntico são capazes de adquirir e expressar resistência aos agentes antimicrobianos comumente empregados para tratar infecções, o que torna necessário, em determinadas situações, a realização de testes laboratoriais para detectar a resistência ou a suscetibilidade antimicrobiana desses micro-organismos. Objetivo: avaliar a suscetibilidade antimicrobiana das cepas de Enterococcus faecalis, Enterococcus faecium, Actinomyces viscosus e Staphylococcus aureus isoladas de canais radiculares de dentes com insucesso endodôntico. Material e método: Cepas clínicas de Enterococcus faecalis (n=3), Enterococcus faecium (n=3), Actinomyces viscosus (n=3) e Staphylococcus aureus (n=3), coletadas in vivo de canais radiculares com insucesso endodôntico, foram testadas quanto à suscetibilidade antimicrobiana por meio do método E-test em duplicata, utilizando os antibióticos: Amoxicilina (AC), Rifampicina (RI), Moxifloxacina (MX), Vancomicina (VA), Tetraciclina (TC), Ciprofloxacina (CI), Cloranfenicol (CL), Benzilpenicilina (PG), Amoxicilina + ácido clavulânico (XL), Doxiciclina (DC), Eritromicina (EM) e Azitromicina (AZ). Resultado: Todas as cepas clínicas testadas foram suscetíveis aos antibióticos AC, XL, PG, DC, MX, TC e VA. Todos os isolados das espécies de S. aureus foram suscetíveis aos 12 antibióticos testados. As cepas de E. faecalis, E. faecium e A. viscosus mostraram padrão de suscetibilidade intermediário contra EM. Algumas cepas de E. faecalis e E. faecium foram resistentes a AZ e RI. Conclusão: As cepas clínicas isoladas dos canais radiculares de dentes com insucesso endodôntico mostraram perfis diferentes de suscetibilidade antimicrobiana e nenhum isolado de E. faecalis e E. faecium apresentou-se suscetível a AZ e EM.Descritores: Endodontia; bactéria; antibióticos. AbstractIntroduction: Bacteria associated with failure endodontic treatment are capable of acquiring and expressing resistance against antimicrobial agents usually used to treat infections, which makes necessary, in some cases, laboratory tests in order to detect the resistance or antimicrobial susceptibility of these microorganisms. Objective: to evaluate the antimicrobial susceptibility of Enterococcus faecalis, Enterococcus faecium, Actinomyces viscosus and Staphylococcus aureus strains isolated from root canals of teeth with failure endodontic treatment. Material and method: Enterococcus faecalis (n=3), Enterococcus faecium (n=3), Actinomyces viscosus (n=3) and Staphylococcus aureus (n=3) clinical strains collected in vivo from root-filled canals with failure endodontic treatment thad been their antimicrobial susceptibility tested by the E-test method in duplicate using the antibiotics: Amoxicillin (AC), Rifampicin (RI), Moxifloxacin (MX), Vancomycin (VA), Tetracycline (TC), Ciprofloxacin (CI), Chloranphenicol (CL), Benzilpenicillin (PG), Amoxicillin + clavulanic acid (XL), Doxycycline (DC), Erythromycin (EM) e Azithromycin (AZ). Result: all the clinical strains tested were susceptib...

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In Vitro Activity of Amoxicillin, Clindamycin, Doxycycline, Metronidazole, and Moxifloxacin Against Oral Actinomyces

Cited by 41 publications

References 30 publications

Antimicrobial Effects of Novel Triple Antibiotic Paste–Mimic Scaffolds on Actinomyces naeslundii Biofilm

Antimicrobial Effects of Novel Triple Antibiotic Paste–Mimic Scaffolds on Actinomyces naeslundii Biofilm

Comparative antimicrobial efficacy of herbal alternatives ( Emblica officinalis , Psidium guajava ), MTAD, and 2.5% sodium hypochlorite against Enterococcus faecalis : An in vitro study

Avaliação da suscetibilidade antimicrobiana de bactérias anaeróbias facultativas isoladas de canais radiculares de dentes com insucesso endodôntico frente aos antibióticos de uso sistêmico

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