In vitro activity of Artemisia annua L (Asteraceae) extracts against Rhipicephalus (Boophilus) microplus

Chagas, Ana Carolina de Souza; Georgetti, C.; Carvalho, Carlos Alberto de; Oliveira, M. C. de S.; Rodrigues, Rodney Alexandre Ferreira; Foglio, Mary Ann; Magalhães, Pedro Melillo de

doi:10.1590/s1984-29612011000100007

Cited by 19 publications

(10 citation statements)

References 17 publications

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“…Hexane extract of A. oleracea was highly effective against larvae of the tick R. microplus, with high mortality at concentrations above 1.6 mg mL −1 and a low LC 50 (0.8 mg mL −1 , CL 95 % 0.7-0.8). These results demonstrate greater efficacy of this extract compared to other plant extracts of the family Asteraceae, such as Calea serrata and Artemisia annua (Ribeiro et al 2008;Chagas et al 2011).…”

Section: Discussionmentioning

confidence: 70%

Acaricide activity in vitro of Acmella oleracea against Rhipicephalus microplus

Castro

Lima²,

Vasconcelos³

et al. 2014

Parasitol Res

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Cattle tick control has been limited by the resistance of these parasites to synthetic acaricides. Natural products are a possible alternative as they have different mechanisms of action. Acmella oleracea is a native plant with a large cultivated area in the Amazon region and could be easily used for large-scale preparation of a commercial product. This study evaluated the in vitro action of the hexane extract of the aerial parts of A. oleracea on larvae and engorged females of the cattle tick Rhipicephalus microplus. Spilanthol was the major constituent with a content of 14.8% in the extract. The hexane extract of A. oleracea was highly effective against larvae of R. microplus with an LC50 of 0.8 mg mL(-1). Against engorged females, hexane extract of A. oleracea reduced oviposition and hatchability of eggs with an LC50 of 79.7 mg mL(-1). Larvae and engorged females were killed by the hexane extract with high efficiency (>95%) at concentrations of 3.1 and 150.0 mg mL(-1), respectively. These results demonstrate that the hexane extract of A. oleracea has significant activity against R. microplus and has potential to be developed into formulations for tick control.

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Section: Discussionmentioning

confidence: 70%

Acaricide activity in vitro of Acmella oleracea against Rhipicephalus microplus

Castro

Lima²,

Vasconcelos³

et al. 2014

Parasitol Res

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“…Studies have previously tested extracts of A. annua in vitro, with results reporting a variety of bioactive properties, including protection against oxidative stress, 24 and antioxidant, 25 anthelminthic, 26 and anti-pest 27 properties. However, to our knowledge, this is the first report of in vitro anti-inflammatory properties in this interesting plant.…”

Section: Discussionmentioning

confidence: 99%

An extract of the medicinal plant Artemisia annua modulates production of inflammatory markers in activated neutrophils

Hunt¹,

Yoshida²,

Davis³

et al. 2015

JIR

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PurposeTo investigate the ability of a commercial extract from the medicinal plant Artemisia annua to modulate production of the cytokine, tumor necrosis factor-alpha (TNF-α), and the cyclooxygenase (COX) inflammatory marker, prostaglandin E2 (PGE2) in activated neutrophils.MethodsNeutrophils were harvested from rat whole blood and cultured in the presence of plant extract or control samples. Neutrophils, except unactivated control cells, were activated with 10 μg/mL lipopolysaccharide (LPS). The cells were cultured with a range of different concentrations of the A. annua extracts (400–1 μg/mL) and artemisinin (200 and 100 μg/mL) and the supernatants were then tested by enzyme-linked immunosorbent assay (ELISA) for the concentrations of TNF-α and PGE2. Each sample was assayed in triplicate. Positive controls with an inhibitor were assayed in triplicate: chloroquine 2.58 and 5.16 μg/mL for TNF-α, and ibuprofen 400 μg/mL for PGE2. An unsupplemented group was also assessed in triplicate as a baseline control.ResultsNeutrophils were stimulated to an inflammatory state by the addition of LPS. A. annua extract significantly inhibited TNF-α production by activated neutrophils in a dose-dependent manner. There was complete inhibition by the A. annua extract at 200, 100, and 50 μg/mL (all P≤0.0003). At A. annua extract concentrations of 25, 10, and 5 μg/mL, TNF-α production was inhibited by 89% (P<0.0001), 54% (P=0.0002), and 38% (P=0.0014), respectively. A. annua 1 μg/mL did not significantly inhibit TNF-α production (8.8%; P>0.05). Concentrations of 400, 200, and 100 μg/mL A. annua extract significantly inhibited PGE2 production by 87% (P=0.0128), 91% (P=0.0017), and 93% (P=0.0114), respectively.ConclusionAn extract of A. annua was shown to be a potent inhibitor of TNF-α and a strong inhibitor of PGE2 production in activated neutrophils at the concentrations tested. Further studies are warranted with this promising plant extract.

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“…Besides a significant reduction ( P < 0.05) in the egg mass (85.1%) at 20% concentration and complete blockage of egg hatchability, 100% larval mortality at 5% concentration of the extract has also been observed. Chagas et al [ 31 ] reported greater efficacy of the extracts of A. annua against R. ( B. ) microplus with EC 50 of 130.6 mg/mL and EC 90 of 302.9 mg/mL.…”

Section: Discussionmentioning

confidence: 99%

“…( B .) microplus by various workers [ 31 – 33 ]. The artemisinin is thought to exert its effect by reacting with the heme groups of the haemoglobin molecules digested by parasites, altering the cell structure and its functions through the free radicles derived from artemisinin, thus affecting the growth and reproduction [ 34 ].…”

Section: Discussionmentioning

confidence: 99%

In Vitro Evaluation of Ethanolic Extracts ofAgeratum conyzoidesandArtemisia absinthiumagainst Cattle Tick,Rhipicephalus microplus

Parveen

Godara

Katoch

et al. 2014

The Scientific World Journal

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In vitro efficacy of ethanolic extracts obtained from the aerial parts of Ageratum conyzoides and Artemisia absinthium was assessed on Rhipicephalus microplus using adult immersion test (AIT). Five concentrations of the extract (1.25%, 2.5%, 5%, 10%, and 20%) with three replications for each concentration were used in the bioassay. In AIT, the maximum mortality was recorded as 40% and 66.7% at 20% concentration for A. conyzoides and A. absinthium, respectively. Acaricidal activity was found to be higher in the extract of A. absinthium with LC50 and LC95 values of 11.2% and 61.7%, respectively. Egg mass weight of the live ticks treated with different concentrations of the extracts was significantly (P < 0.05) lower than that of control ticks; consequently, the reproductive index and oviposition values of the treated ticks were reduced significantly (P < 0.05). The A. conyzoides inhibited 90% hatching of eggs at the 20% concentration, whereas A. absinthium showed 100% inhibition at 5%, 10%, and 20% concentrations. The results show that A. absinthium has better acaricidal properties than A. conyzoides and could be useful in controlling R. microplus.

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In vitro activity of Artemisia annua L (Asteraceae) extracts against Rhipicephalus (Boophilus) microplus

Cited by 19 publications

References 17 publications

Acaricide activity in vitro of Acmella oleracea against Rhipicephalus microplus

Acaricide activity in vitro of Acmella oleracea against Rhipicephalus microplus

An extract of the medicinal plant Artemisia annua modulates production of inflammatory markers in activated neutrophils

In Vitro Evaluation of Ethanolic Extracts ofAgeratum conyzoidesandArtemisia absinthiumagainst Cattle Tick,Rhipicephalus microplus

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