In vitro activity of essential oils extracted from condiments against fluconazole-resistant and -sensitive Candida glabrata

Soares, Isaura Helena; Loreto, Érico S.; Rossato, Luana; Mário, Débora Nunes; Venturini, Tarcieli Pozzebon; Baldissera, Fernanda Giesel; Santúrio, Jânio Morais; Alves, Sydney Hartz

doi:10.1016/j.mycmed.2015.06.003

Cited by 25 publications

(29 citation statements)

References 14 publications

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“…The main finding was that the susceptibility of FLU-resistant C. albicans to EOs was higher than those of the FLU-susceptible yeasts. Similarly, Soares et al [35] confirmed the antifungal activity of EOs from OR, TY and cinnamon against FLU-susceptible and FLU-resistant Candida glabrata. In yeast, various mechanisms of cell destruction have been observed in EOs.…”

Section: Discussionmentioning

confidence: 74%

“…An amount of 10 µL of each EO, at a concentration 100%, was applied on a sterile paper disc (6 mm Ø Whatman No.1) aseptically placed on the inoculated plates [11]. The average of inhibition zones was evaluated classifying the EOs as follows: not sensitive for a diameter smaller than 8 mm; sensitive for a diameter of 9-14 mm; very sensitive for a diameter of 15-19 mm; extremely sensitive for a diameter larger than 20 mm [35]. Cefuroxime (30 µg/disc; Sigma-Aldrich., Saint-Louis, MO, USA) and fluconazole (20 µg/disc; Sigma-Aldrich, Saint-Louis, MO, USA) were used as positive controls for bacterial and fungal inhibition.…”

Section: Eos Agar Disc Diffusion Assaymentioning

confidence: 99%

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The Effect of Ten Essential Oils on Several Cutaneous Drug-Resistant Microorganisms and Their Cyto/Genotoxic and Antioxidant Properties

et al. 2019

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In this study, we determined the antimicrobial activity of ten essential oils (EOs)—oregano, thyme, clove, arborvitae, cassia, lemongrass, melaleuca, eucalyptus, lavender, and clary sage—against drug-resistant microorganisms previously isolated from patients with skin infections. The essential oil compositions were determined using gas chromatography coupled to mass spectrometry (GC/MS). The assayed bacteria included Pseudomonas aeruginosa, Proteus vulgaris, Citrobacter koseri, and Klebsiella pneumoniae. Two drug-resistant yeasts (Candida albicans and Candida parapsilosis) were also involved in our survey. Oregano, thyme, cassia, lemongrass and arborvitae showed very strong antibacterial and antifungal activity against all tested strains. These results show that these essential oils may be effective in preventing the growth of the drug-resistant microorganisms responsible for wound infections. In this study, the genotoxic effects of tested essential oils on healthy human keratinocytes HaCaT were evaluated using the comet assay for the first time. These results revealed that none of the essential oils induced significant DNA damage in vitro after 24 h. Moreover, the treatment of HaCaT cells with essential oils increased the total antioxidant status (TAS) level. The obtained results indicate that EOs could be used as a potential source of safe and potent natural antimicrobial and antioxidant agents in the pharmaceutical and food industries.

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Section: Discussionmentioning

confidence: 74%

Section: Eos Agar Disc Diffusion Assaymentioning

confidence: 99%

The Effect of Ten Essential Oils on Several Cutaneous Drug-Resistant Microorganisms and Their Cyto/Genotoxic and Antioxidant Properties

et al. 2019

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“…High antifungal activity of examined EOs, also against antibiotic-resistant isolates is according to recent evidence of some authors [20, 27, 28]. However, it is difficult to compare the data with the literature because the antimicrobial activity of EOs and their components are influenced by the several factors including chemical compositions and experimental conditions.…”

Section: Resultsmentioning

confidence: 88%

“…In literature, there are some available data referring to the anticandidal activity of EOs such as thyme, clove, pine, fennel, sage, and lemon balm [5–7, 19, 20]. …”

Section: Resultsmentioning

confidence: 99%

Liquid and vapour-phase antifungal activities of essential oils against Candida albicans and non-albicans Candida

Mandras

Nostro

Roana

et al. 2016

BMC Complement Altern Med

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BackgroundThe management of Candida infections faces many problems, such as a limited number of antifungal drugs, toxicity, resistance of Candida to commonly antifungal drugs, relapse of Candida infections, and the high cost of antifungal drugs. Though azole antifungal agents and derivatives continue to dominate as drugs of choice against Candida infections, there are many available data referring to the anticandidal activity of essential oils. Since we have previous observed a good antimicrobial activity of some essential oils against filamentous fungi, the aim of this study was to extend the research to evaluate the activity of the same oils on Candida albicans, C.glabrata and C.tropicalis clinical strains, as well as the effects of related components. Essential oils selection was based both on ethnomedicinal use and on proved antibacterial and/or antifungal activity of some of these oils. Fluconazole and voriconazole were used as reference drugs.MethodsThe minimum inhibitory concentration (MIC) and the minimal fungicidal concentration (MFC) of essential oils (thyme red, fennel, clove, pine, sage, lemon balm, and lavender) and their major components were investigated by the broth microdilution method (BM) and the vapour contact assay (VC).ResultsUsing BM, pine oil showed the best activity against all strains tested, though C.albicans was more susceptible than C.glabrata and C.tropicalis (MIC50-MIC90 = 0.06 %, v/v). On the contrary, sage oil displayed a weak activity (MIC50-MIC90 = 1 %, v/v). Thyme red oil (MIC50-MIC90 ≤ 0.0038 %, v/v for C.albicans and C.tropicalis, and 0.0078- < 0.015 %, v/v for C.glabrata), followed by lemon balm, lavender and sage were the most effective by VC. Carvacrol and thymol showed the highest activity, whereas linalyl acetate showed the lowest activity both by two methods. α-pinene displayed a better activity by BM than VC.ConclusionResults show a good activity of essential oils, mainly thymus red and pine oils, and their components carvacrol, thymol and α-pinene against Candida spp., including fluconazole/voriconazole resistant strains. These data encourage adequately controlled and randomized clinical investigations. The use in vapour phase could have additional advantages without requiring direct contact, resulting in easy of environmental application such as in hospital, and/or in school.

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“…One of the most promising natural alternatives to traditional antifungal preparations are the essential oils (EOs) drawn from medicinal plants. Many of the natural products containing EOs and their constituents have antimicrobial properties, while several studies have shown high sensitivity of pathogenic fungi, including yeasts, to EOs [2,[5][6][7][8]. According to Waller et al [9][10][11], 55 botanical species belonging to 27 genus exhibit antifungal effects, especially the aromatic plants, notably Origanum vulgare L., Satureja hortensis L., Thymus serpyllum L. and Thymus vulgaris L. from the Lamiaceae family.…”

Section: Introductionmentioning

confidence: 99%

Chemical composition and in vitro activity of Origanum vulgare L., Satureja hortensis L., Thymus serpyllum L. and Thymus vulgaris L. essential oils towards oral isolates of Candida albicans and Candida glabrata

et al. 2020

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AbstractThe purpose of this research was to investigate the chemical composition of essential oils (EOs) from: Origanum vulgare L., Satureja hortensis L., Thymus serpyllum L. and Thymus vulgaris L. (Lamiaceae) cultivated in Poland, and to study their antifungal activity towards clinical isolates of oral Candida spp. The hydrodistilled essential oils were analyzed using the GC-MS method. The antifungal activity was evaluated in vitro against oral isolates and reference strains of Candida albicans and C. glabrata, using the broth microdilution method according to the European Committee on Antimicrobial Susceptibility Testing (EUCAST) and Clinical and Laboratory Standards Institute (CLSI) guidelines, allowing for estimation of minimal inhibitory concentration (MIC) and minimal fungicidal concentration (MFC). GC-MS analysis revealed that carvacrol was the main EO compound in oregano and summer savory, while thymol and linalool were the major ingredients of thyme and wild thyme oils, respectively. The EOs possessed fungicidal activity against C. albicans and C. glabrata, including oral isolates, with MIC = 125 – 2000 mg/L, MFC = 250 – 4000 mg/L and MFC/MIC = 1 – 4, depending on the yeast and plant species. The most active was thyme oil – with MIC = 125 – 500 mg/L, MFC = 250 – 500 mg/L and MFC/MIC = 1 – 2.

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In vitro activity of essential oils extracted from condiments against fluconazole-resistant and -sensitive Candida glabrata

Cited by 25 publications

References 14 publications

The Effect of Ten Essential Oils on Several Cutaneous Drug-Resistant Microorganisms and Their Cyto/Genotoxic and Antioxidant Properties

The Effect of Ten Essential Oils on Several Cutaneous Drug-Resistant Microorganisms and Their Cyto/Genotoxic and Antioxidant Properties

Liquid and vapour-phase antifungal activities of essential oils against Candida albicans and non-albicans Candida

Chemical composition and in vitro activity of Origanum vulgare L., Satureja hortensis L., Thymus serpyllum L. and Thymus vulgaris L. essential oils towards oral isolates of Candida albicans and Candida glabrata

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