In vitro activity of pentamidine against Tropheryma whipplei

Rolain, Jean‐Marc; Fénollar, Florence; Raoult, Didier

doi:10.1016/j.ijantimicag.2011.07.015

Cited by 6 publications

(2 citation statements)

References 15 publications

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“…This medium consisted of cell culture medium that provided the missing amino acids, supplemented with 10% fetal calf serum, 1% glutamine, and 1% human nonessential amino acids (13). This approach permitted the successful growth of T. whipplei from a great variety of clinical samples as well as antibiotic susceptibility testing (4,373). The development of an axenic medium permitted the culturing of isolates from sterile and nonsterile sources, such as saliva and stool samples, after a decontamination procedure with glutaraldehyde (312) or with filtration (374,375).…”

Section: Axenic Culture Media For Intracellular Bacteriamentioning

confidence: 99%

Current and Past Strategies for Bacterial Culture in Clinical Microbiology

et al. 2015

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SUMMARY A pure bacterial culture remains essential for the study of its virulence, its antibiotic susceptibility, and its genome sequence in order to facilitate the understanding and treatment of caused diseases. The first culture conditions empirically varied incubation time, nutrients, atmosphere, and temperature; culture was then gradually abandoned in favor of molecular methods. The rebirth of culture in clinical microbiology was prompted by microbiologists specializing in intracellular bacteria. The shell vial procedure allowed the culture of new species of Rickettsia . The design of axenic media for growing fastidious bacteria such as Tropheryma whipplei and Coxiella burnetii and the ability of amoebal coculture to discover new bacteria constituted major advances. Strong efforts associating optimized culture media, detection methods, and a microaerophilic atmosphere allowed a dramatic decrease of the time of Mycobacterium tuberculosis culture. The use of a new versatile medium allowed an extension of the repertoire of archaea. Finally, to optimize the culture of anaerobes in routine bacteriology laboratories, the addition of antioxidants in culture media under an aerobic atmosphere allowed the growth of strictly anaerobic species. Nevertheless, among usual bacterial pathogens, the development of axenic media for the culture of Treponema pallidum or Mycobacterium leprae remains an important challenge that the patience and innovations of cultivators will enable them to overcome.

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Section: Axenic Culture Media For Intracellular Bacteriamentioning

confidence: 99%

Current and Past Strategies for Bacterial Culture in Clinical Microbiology

et al. 2015

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“…Serious human health problems caused by these diseases in developing countries as well as after the spread of AIDS have stimulated research to find new drugs against them. There are several indications that pentamidine has a wider range of antimicrobial activity [ 10 , 11 ], however its high activity is associated with high toxicity and low bioavailability [ 12 ]. Recently we have found that some pentamidine analogs had little to no toxicity [ 13 ], and although they are not as quite potent as pentamidine, they hold promise for a decreased side effect within mammalian host.…”

Section: Introductionmentioning

confidence: 99%

Probing the Relationship between Anti-Pneumocystis carinii Activity and DNA Binding of Bisamidines by Molecular Dynamics Simulations

Żołek¹,

Maciejewska²,

Żabiński³

et al. 2015

Molecules

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The anti-Pneumocystis carinii activity of 13 synthetic pentamidine analogs was analyzed. The experimental differences in melting points of DNA dodecamer 5'-(CGCGAATTCGCG)2-3' complexes (ΔTm), and in the biological activity measured using ATP bioluminescence assay (IC50) together with the theoretical free energy of DNA-ligand binding estimated by the proposed computational protocol, showed that the experimental activity of the tested pentamidines appeared to be due to the binding to the DNA minor groove with extended AT sequences. The effect of heteroatoms in the aliphatic linker, and the sulfonamide or methoxy substituents on the compound inducing changes in the interactions with the DNA minor groove was examined and was correlated with biological activity. In computational analysis, the explicit solvent approximation with the discrete water molecules was taken into account, and the role of water molecules in the DNA-ligand complexes was defined.

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Tropheryma whipplei

Liu

Austin

2015

Molecular Medical Microbiology

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In vitro activity of pentamidine against Tropheryma whipplei

Cited by 6 publications

References 15 publications

Current and Past Strategies for Bacterial Culture in Clinical Microbiology

Current and Past Strategies for Bacterial Culture in Clinical Microbiology

Probing the Relationship between Anti-Pneumocystis carinii Activity and DNA Binding of Bisamidines by Molecular Dynamics Simulations

Tropheryma whipplei

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