In-vitro activity of solithromycin against anaerobic bacteria from the normal intestinal microbiota

Weintraub, Andrej; Rashid, Mamun Ur; Nord, Carl Erik

doi:10.1016/j.anaerobe.2016.10.002

Cited by 7 publications

(5 citation statements)

References 15 publications

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“…In an infant with E. coli and B. fragilis mixed infectious meningitis (Ganeshalingham et al 2014 ), B. fragilis was confirmed resistant to ceftriaxone. In an in-vitro susceptibility test (Weintraub et al 2016 ), Prevotella isolates were susceptible to metronidazole but resistant to ceftriaxone. It is well-known that metronidazole had a very good activity against most strict anaerobes, including Prevotella (Bancescu et al 2015 ).…”

Section: Discussionmentioning

confidence: 99%

A chinese case of prevotella intermedia and streptococcus constellatus intracranial mixed infection

Liuhua

Chen

et al. 2017

Metab Brain Dis

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Streptococcal Species is increasingly recognized as a potentially preventable emerging infection in human’s brain with high prevalence around the world. Streptococcus constellatus is one of the most common pathogens. Meanwhile, anaerobic bacteria are the rare causes for intracranial infection. To date, intracranial mixed infection caused by Prevotella intermedia and Streptococcus constellatus has not been reported. We reported a Chinese case to raise the global awareness of severity of the intracranial mixed infection. Here, we illustrated the epidemiological risk factors, clinical manifestations and outcomes of the patient. For patients who suffer from exacerbated brain infection with fetid cerebrospinal fluid, early repeated imaging is urgently needed and empiric antibiotic therapy should consider anaerobic and aerobic bacteria in these situations.

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Section: Discussionmentioning

confidence: 99%

A chinese case of prevotella intermedia and streptococcus constellatus intracranial mixed infection

Liuhua

Chen

et al. 2017

Metab Brain Dis

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“…Azithromycin was the most active antibiotic tested against the gram-negative anaerobes (Williams et al, 1992). Compared with a MIC 90 against azithromycin ranging from 2.0 to 256 mg/L to different species of LAB (Weintraub et al, 2016), the 3 strains all showed moderate resistance, with MIC of 20 mg/L. The MIC of the investigated strains against cephalexin was 640 mg/L, showing much higher resistance than either the DSM 20021T strain or Lacticaseibacillus rhamnosus GG (Coppola et al, 2005).…”

Section: Antibiotic Susceptibilitymentioning

confidence: 97%

Isolation and characterization of lactic acid bacteria from human milk

Kang

Pan²,

Peng³

et al. 2020

Journal of Dairy Science

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Human milk is the main source of nutrition for infants and the transmission of various microorganisms. The lactic acid bacteria (LAB) in breast milk allow for the establishment of the gut microflora of infants. In this study, we aimed to assess the probiotic potential of LAB strains isolated from breast milk of healthy Chinese women. Two strains, Lacticaseibacillus rhamnosus (formerly Lactobacillus rhamnosus) LHL6 and LHL7, were selected and identified through morphology observation, Gram staining, and 16S rDNA phylogenetic analysis. Using Limosilactobacillus fermentum (formerly Lactobacillus fermentum) CECT5716 as the standard reference strain, the screened strains were characterized for aspects of growth, production of lactic acid and H 2 O 2 , antibiotic susceptibility, survival under simulated gastrointestinal conditions, and tolerance to cadmium (Cd). In de Man, Rogosa, and Sharpe (MRS) broth, LHL6 and LHL7 showed longer lag phases than CECT5716 but higher specific growth rates. For the production of lactic acid and H 2 O 2 , LHL7 performed better than LHL6 and CECT5716, indicating better antimicrobial ability. Strain LHL7 generated 9.99 mg/L H 2 O 2 , considerably higher than 1.25 mg/L for LHL6 and 2.33 mg/L for CECT5716. According to European Food Safety Authority minimum inhibitory concentrations, all of the investigated strains were resistant to chloramphenicol, streptomycin, and kanamycin. However, unlike LHL6 and CECT5716, LHL7 was susceptible to ampicillin and resistant to tetracycline. Resistance to azithromycin, cephalexin, and penicillin G were similar for all 3 strains, whereas CECT5716 was resistant to a higher concentration of roxithromycin. All 3 strains were able to survive in a simulated gastric-like solu-tion, but a low percentage survived in the presence of 0.4% bile salt and 7% pancreatin. Encapsulation with protectants may enhance the survival rate. All 3 strains were tolerant to 500 mg/L Cd in MRS broth and to 1,000 mg/L Cd on MRS agar medium. In summary, 2 novel strains of LAB were obtained that have similar characteristics to the reference strain CECT5716. This work identified potential probiotic candidates for application in the food and pharmaceutical industries and facilitated identification of further probiotics.

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“…The in vitro activity of AZM on the genus Prevotella has been proven for a long time with modifications of microbiota [142] and low MICs [143][144][145][146][147][148][149][150][151], providing bactericidal activity [152] and including β-lactamaseproducing strains of Prevotella spp [149,153]. But it was reported that the MICs varied mostly according to strains [154] with ranges of MIC between ≤0.02 and >256, whether by dilution in agar medium [155][156][157] or by the E test method [158,159] or by disc diffusion method [160], and to differences in the susceptibility profiles in separate countries [161]. Resistome analysis of the gut microbiome of preschool children from 30 Nigerian communities randomized to either biannual AZM or placebo showed an increase in macrolide resistance gene expression in communities treated with AZM (P=0.004).…”

Section: Immunological Argumentsmentioning

confidence: 99%

SARS-CoV-2 and Prevotella spp.: friend or foe? A systematic literature review

Tamanai-Shacoori

Gall‐David

Moussouni

et al. 2022

Journal of Medical Microbiology

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During this global pandemic of the COVID-19 disease, a lot of information has arisen in the media and online without scientific validation, and among these is the possibility that this disease could be aggravated by a secondary bacterial infection such as Prevotella, as well as the interest or not in using azithromycin, a potentially active antimicrobial agent. The aim of this study was to carry out a systematic literature review, to prove or disprove these allegations by scientific arguments. The search included Medline, PubMed, and Pubtator Central databases for English-language articles published 1999–2021. After removing duplicates, a total of final eligible studies (n=149) were selected. There were more articles showing an increase of Prevotella abundance in the presence of viral infection like that related to Human Immunodeficiency Virus (HIV), Papillomavirus (HPV), Herpesviridae and respiratory virus, highlighting differences according to methodologies and patient groups. The arguments for or against the use of azithromycin are stated in light of the results of the literature, showing the role of intercurrent factors, such as age, drug consumption, the presence of cancer or periodontal diseases. However, clinical trials are lacking to prove the direct link between the presence of Prevotella spp. and a worsening of COVID-19, mainly those using azithromycin alone in this indication.

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In-vitro activity of solithromycin against anaerobic bacteria from the normal intestinal microbiota

Cited by 7 publications

References 15 publications

A chinese case of prevotella intermedia and streptococcus constellatus intracranial mixed infection

A chinese case of prevotella intermedia and streptococcus constellatus intracranial mixed infection

Isolation and characterization of lactic acid bacteria from human milk

SARS-CoV-2 and Prevotella spp.: friend or foe? A systematic literature review

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