In vitro activity of the β-carboline alkaloids harmane, harmine, and harmaline toward parasites of the species Leishmania infantum

Giorgio, Carole Di; Delmas, F.; Ollivier, Évelyne; Élias, Riad; Balansard, G.; Timon‐David, P.

doi:10.1016/j.exppara.2004.04.002

Cited by 144 publications

(80 citation statements)

References 36 publications

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“…Hairy root cultures of Oxalis tuberose transformed with A. rhizogenes (ATCC-15834) showed better growth and exudation of harmine and harmaline in comparison to untransformed control roots. Harmine and Harmaline showed wide constitutive antimicrobial activity against soil-borne microorganisms (Bais et al 2003), anti-leishmanial activity (Di Giorgio et al 2004), bioinsecticidal activity (Rharrabe et al 2007), and cytotoxic to cancer cells (Rivas et al 1999). HPLC analysis of hairy roots clones of Hyoscyamus and Persian poppy of Papaver bracteatum L. showed that the relative concentration of alkaloids were virtually identical in wild-type and transgenic roots.…”

Section: Discussionmentioning

confidence: 99%

The influence of Agrobacterium rhizogenes on induction of hairy roots and ß-carboline alkaloids production in Tribulus terrestris L.

Sharifi

Sattari

Zebarjadi

et al. 2013

Physiol Mol Biol Plants

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We have developed an efficient transformation system for Tribulus terrestris L., an important medicinal plant, using Agrobacterium rhizogenes strains AR15834 and GMI9534 to generate hairy roots. Hairy roots were formed directly from the cut edges of leaf explants 10-14 days after inoculation with the Agrobacterium with highest frequency transformation being 49 %, which was achieved using Agrobacterium rhizogenes AR15834 on hormone-free MS medium after 28 days inoculation. PCR analysis showed that rolB genes of Ri plasmid of A. rhizogenes were integrated and expressed into the genome of transformed hairy roots. Isolated transgenic hairy roots grew rapidly on MS medium supplemented with indole-3-butyric acid. They showed characteristics of transformed roots such as fast growth and high lateral branching in comparison with untransformed roots. Isolated control and transgenic hairy roots grown in liquid medium containing IBA were analyzed to detect ß-carboline alkaloids by High Performance Thin Layer Chromatograghy (HPTLC).Harmine content was estimated to be 1.7 μg g −1 of the dried weight of transgenic hairy root cultures at the end of 50 days of culturing. The transformed roots induced by AR15834 strain, spontaneously, dedifferentiated as callus on MS medium without hormone. Optimum callus induction and shoot regeneration of transformed roots in vitro was achieved on MS medium containing 0.4 mg L −1 naphthaleneacetic acid and 2 mg L −1 6-benzylaminopurine (BAP) after 50 days. The main objective of this investigation was to establish hairy roots in this plant by using A. rhizogenes to synthesize secondary products at levels comparable to the wild-type roots.

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Section: Discussionmentioning

confidence: 99%

The influence of Agrobacterium rhizogenes on induction of hairy roots and ß-carboline alkaloids production in Tribulus terrestris L.

Sharifi

Sattari

Zebarjadi

et al. 2013

Physiol Mol Biol Plants

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“…Harmaline can induce spasmolytic effects with muscarinic, histaminic and β-adreno-receptors (Aitadafoun et al, 1996); while the vasorelaxant activity of harmane and harmaline is due to its ability to induce endothelial cells to release NO, and the vascular smooth muscles to inhibit the contractions induced by Ca2 + channels (Shi et al, 2001). Harmane and harmine had moderate antiproliferative activity toward human monocytes and is non-toxic to human cells, exerted strong activity against the intracellular amastigote of Leishmania infantum partly due to its capacity to inhibit leishmanial PKC activity (Di Giorgio et al, 2004). This beta-carboline alkaloid also has scavenging action on reactive oxygen species and inhibits thiol oxidation (Kim et al, 2001).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of analgesic and antiinflammatory activity of Ophiorrhiza nicobarica, an ethnomedicine from Nicobar Islands, India

Chattopadhyay¹,

Das²,

Mandal³

et al. 2007

Oriental Pharmacy and Experimental Medicine

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SUMMARYThis study reports the analgesic, anti-inflammatory and membrane-stabilizing property of alcoholic extract of Ophiorrhiza nicobarica (ON), a wild herb, used as an anti-infective ethnomedicine of Nicobarese and Shompen tribes of Great Nicobar Island, India. We for the first time investigated the analgesic and antiinflammatory potential of this herb in acute, subacute and chronic model of inflammation in Swiss albino mice and Wistar albino rats, along with sheep RBC-induced sensitivity and membrane stabilization. The acetic acid induced writhing, tail flick and tail immersion tests are used as a model for evaluating analgesic activity; while the carrageenin-induced paw oedema was used as the model for acute inflammation, dextran-induced oedema as sub-acute and cotton-pellateinduced granuloma as chronic inflammatory model. The probable mode by which ON mediate its effect on inflammatory conditions was studied on sheep RBC-induced sensitivity and membrane stabilization. The in vitro results revealed that the ON extract possesses significant (P < 0.05) dose dependent analgesic and antiinflammatory activity at 200 and 300 mg/kg and its fractions at 50 mg/ kg, p.o. respectively, compared to the control groups. However, the extract failed to exhibit membrane-stabilizing property as it unable to reduce the level of haemolysis of RBC exposed to hypotonic solution. The acute toxicity studies of ON extract in rats and mice revealed that the extract was nontoxic even up to 3.0 g/kg body weight of the animals, with a high safety profile. We have isolated ursolic acid, β-sitosterol and harmaline respectively, from the bioactive part of the extract. The results indicated that the O. nicobarica is indeed beneficial in primary health care, and suggest that its anti-inflammatory activity may not be related to membrane-stabilization.

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“…They also have hallucinogenic and antioxidative properties 3,4 . Although harmane has not yet been used as the active principle of any commercial medicine, studies have indicated that this substance has antileishmanial and anti-HIV activity [5][6][7] .…”

Section: Introductionmentioning

confidence: 99%

Heavy atom enhanced room-temperature phosphorimetry for ultratrace determination of harmane

2008

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Harmane has been proposed for the treatment of epilepsy, AIDS and leshmaniosis. Its room-temperature phosphorescence was induced using either AgNO 3 or TlNO 3 , enabling absolute limits of detection of 0.12 and 2.4 ng respectively, with linear dynamic ranges extending up to 456 ng (AgNO 3 ) and 911 ng (TlNO 3 ). Relative standard deviations around 3% were observed for substrates containing 46 ng of harmane. Such sensitivity and precision are needed because harmane intake must be strictly controlled to achieve proper therapeutic response. Interference studies were performed using thalidomide, reserpine and yohimbine. Recovery of 104±6% was achieved using solid surface room-temperature phosphorimetry. The result was comparable to the one obtained by micellar electrokinetic chromatography.

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In vitro activity of the β-carboline alkaloids harmane, harmine, and harmaline toward parasites of the species Leishmania infantum

Cited by 144 publications

References 36 publications

The influence of Agrobacterium rhizogenes on induction of hairy roots and ß-carboline alkaloids production in Tribulus terrestris L.

The influence of Agrobacterium rhizogenes on induction of hairy roots and ß-carboline alkaloids production in Tribulus terrestris L.

Evaluation of analgesic and antiinflammatory activity of Ophiorrhiza nicobarica, an ethnomedicine from Nicobar Islands, India

Heavy atom enhanced room-temperature phosphorimetry for ultratrace determination of harmane

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