In vitro adherence properties of Lactobacillus rhamnosus DR20 and Bifidobacterium lactis DR10 strains and their antagonistic activity against an enterotoxigenic Escherichia coli

Gopal, Pramod K.; Prasad, Jaya; Smart, James L.; Gill, H. S.

doi:10.1016/s0168-1605(01)00440-8

Cited by 326 publications

(216 citation statements)

References 26 publications

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“…An antagonistic activity against adhesion and invasion of ETEC on Caco-2 and HT-29 cells by spent culture supernatant of L. rhamnosus and B. lactis has also been reported in other studies [24]. Since the molecules secreted by these probiotics in the medium were affected by lactate dehydrogenase, trypsin and proteinase K, the results indicated a synergistic action of lactic acid and proteinaceous substances.…”

Section: Inhibition Of Pathogen Adhesionsupporting

confidence: 75%

Alternatives to in-feed antibiotics in pigs: Evaluation of probiotics, zinc or organic acids as protective agents for the intestinal mucosa. A comparison of in vitro and in vivo results

et al. 2005

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-Weaned pigs are susceptible to frequent infectious diseases. Antibiotics have been used over decades to reduce pathogen infections, but many microorganisms are becoming resistant to antibiotics. Thus, there is an urgent need to find alternatives to in-feed antibiotics. Up to now, various compounds have been used as alternatives, giving promising but sometimes contrasting results. In recent years, the in vitro models of cell culture have been proposed for the screening of various compounds and understanding their mechanisms of action. The intestine is a complex system with a continuous cross-talk among epithelial cells, the local immune system and microflora. Alternatives to in-feed antibiotics may interact with any of these constituents. This review presents data on the protective effects of probiotics, zinc salts and organic acids, used as alternatives to in-feed antibiotics, against pathogen induced damage and inflammatory processes in the intestinal cells and the underlying mechanisms, with an attempt to compare in vitro and in vivo results. All the alternatives tested resulted in effective protection against pathogens by a series of interactions with intestinal cells and pathogens, including the inhibition of adhesion and invasion of pathogens, interference in signalling pathways induced by pathogenic bacteria, maintenance of the epithelial cell cytoskeleton and junctional structure and modulation of the host immune response. microorganismes sont devenus résistants à ces substances. Aussi, est-il urgent de trouver des alternatives aux antibiotiques additifs alimentaires. Jusqu'à présent, diverses alternatives ont été envisagées, certaines donnant des résultats prometteurs, d'autres des résultats variables. Au cours des dernières années, les modèles in vitro de cultures cellulaires ont été proposés pour cribler ces substances et élucider leurs mécanismes d'action. L'intestin est un système complexe faisant intervenir des communications permanentes entre les cellules épithéliales, le système immunitaire local et la microflore. Les alternatives aux antibiotiques peuvent interagir avec n'importe lequel de ces composants. Le présent article fait la synthèse des effets protecteurs et des mécanismes d'action des probiotiques, des sels de zinc et des acides organiques, utilisés comme alternatives aux antibiotiques dans les processus inflammatoires et les altérations cellulaires épithéliales in vitro, induites par des agents pathogènes. Elle tente de comparer ces résultats avec les observations in vivo. Toutes les alternatives évaluées ont été efficaces contre les agents pathogènes, via des mécanismes d'interaction cellules épithéliales-agents pathogènes. Ces mécanismes incluaient l'inhibition de l'adhésion et de l'invasion cellulaire par ces pathogènes, la modulation des mécanismes de signalisation intracellulaire induits par les bactéries pathogènes, le maintien de la structure du cytosquelette et des jonctions serrées des cellules épithéliales, et enfin la modulation des réponses immunitaires de la cellu...

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Section: Inhibition Of Pathogen Adhesionsupporting

confidence: 75%

Alternatives to in-feed antibiotics in pigs: Evaluation of probiotics, zinc or organic acids as protective agents for the intestinal mucosa. A comparison of in vitro and in vivo results

et al. 2005

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“…This result is in line with the fact that the quantity of the administered probiotic bacteria represents only a tiny fraction of the total microbial cell numbers in the intestine, and therefore, it is perhaps unlikely that such administration will change the balance of the major bacterial groups. However, since the administered probiotic strains adhere strongly to human mucus and epithelial cells (Buck et al 2005;Gopal et al 2001), administration of these strains may result in changes in the mucosa-adherent microbiota of the intestine. The mucosa-adherent microbiota is different from the luminal and fecal microbiota (Eckburg et al 2005), and while mucosal microbiota represents only a minor fraction of the total intestinal microbiota, this fraction is likely to have a critically important role in host-microbe interactions including the interactions Fig.…”

Section: Discussionmentioning

confidence: 99%

Probiotic cheese containing Lactobacillus rhamnosus HN001 and Lactobacillus acidophilus NCFM® modifies subpopulations of fecal lactobacilli and Clostridium difficile in the elderly

Lahtinen

Forssten

Aakko

et al. 2011

AGE

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Aging is associated with alterations in the intestinal microbiota and with immunosenescence. Probiotics have the potential to modify a selected part of the intestinal microbiota as well as improve immune functions and may, therefore, be particularly beneficial to elderly consumers. In this randomized, controlled cross-over clinical trial, we assessed the effects of a probiotic cheese containing Lactobacillus rhamnosus HN001 and Lactobacillus acidophilus NCFM on the intestinal microbiota and fecal immune markers of 31 elderly volunteers and compared these effects with the administration of the same cheese without probiotics. The probiotic cheese was found to increase the number of L. rhamnosus and L. acidophilus NCFM in the feces, suggesting the survival of the strains during the gastrointestinal transit. Importantly, probiotic cheese administration was associated with a trend towards lower counts of Clostridium difficile in the elderly, as compared with the run-in period with the plain cheese. The effect was statistically significant in the subpopulation of the elderly who harbored C. difficile at the start of the study. The probiotic cheese was not found to significantly alter the levels of the major microbial groups, suggesting that the microbial changes conferred by the probiotic cheese were limited to specific bacterial groups. Despite that the administration of the probiotic cheese to the study population has earlier been shown to significantly improve the innate immunity of the elders, we did not observe measurable changes in the fecal immune IgA concentrations. No increase in fecal calprotectin and β-defensin concentrations suggests that the probiotic treatment did not affect intestinal inflammatory markers. In conclusion, the administration of probiotic cheese containing L. rhamnosus HN001 and L. acidophilus NCFM, was associated with specific changes in the intestinal microbiota, mainly affecting specific subpopulations of intestinal lactobacilli and C. difficile, but did not have significant effects on the major microbial groups or the fecal immune markers.

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“…The antagonistic activity of LAB living in the GI tract should defend against the invasion of pathogenic microorganisms. [23][24][25] For example, Mukai et al demonstrated an in vitro competition between L. reuteri and Helicobacter pylori for the same receptors, such as gangliotetraosylceramide and sulfatide. 26) In the case of MapA, this protein shows high relatedness to CnBP of L. reuteri NCIB 11951 19) and BspA of L. fermentum BR11.…”

Section: Discussionmentioning

confidence: 99%

A Mucus Adhesion Promoting Protein, MapA, Mediates the Adhesion ofLactobacillus reuterito Caco-2 Human Intestinal Epithelial Cells

Miyoshi

Okada

Uchimura

et al. 2006

Bioscience, Biotechnology, and Biochemistry

115

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In vitro adherence properties of Lactobacillus rhamnosus DR20 and Bifidobacterium lactis DR10 strains and their antagonistic activity against an enterotoxigenic Escherichia coli

Cited by 326 publications

References 26 publications

Alternatives to in-feed antibiotics in pigs: Evaluation of probiotics, zinc or organic acids as protective agents for the intestinal mucosa. A comparison of in vitro and in vivo results

Alternatives to in-feed antibiotics in pigs: Evaluation of probiotics, zinc or organic acids as protective agents for the intestinal mucosa. A comparison of in vitro and in vivo results

Probiotic cheese containing Lactobacillus rhamnosus HN001 and Lactobacillus acidophilus NCFM® modifies subpopulations of fecal lactobacilli and Clostridium difficile in the elderly

A Mucus Adhesion Promoting Protein, MapA, Mediates the Adhesion ofLactobacillus reuterito Caco-2 Human Intestinal Epithelial Cells

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