In vitro administration of sodium arsenite in mouse prepubertal testis induces germ cell loss and apoptosis

Anwar, Naureen; Qureshi, Irfan; Spears, Norah; Lopes, Federica

doi:10.1016/j.tiv.2020.104924

Cited by 5 publications

(8 citation statements)

References 53 publications

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“…Vacuolisation, acidophilic cells, and epithelial degeneration were associated with increased inflammatory cytokines in male rats exposed to sodium arsenite through drinking water [125]. Moreover, sodium arsenite exhibits severe damage to the testicular structure and elevated cleaved caspase 3 (CC3) which is an apoptotic marker in the cluster differentiation 1 (CD1) mouse testes cell line [126]. Increased expression of CC3 also indicates increased apoptosis.…”

Section: Effects Of Arsenic On the Male Reproductive System In Animalsmentioning

confidence: 99%

Contemporary Comprehensive Review on Arsenic-Induced Male Reproductive Toxicity and Mechanisms of Phytonutrient Intervention

Rachamalla

Chinthada

Kushwaha

et al. 2022

Toxics

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Arsenic (As) is a poisonous metalloid that is toxic to both humans and animals. Drinking water contamination has been linked to the development of cancer (skin, lung, urinary bladder, and liver), as well as other disorders such as diabetes and cardiovascular, gastrointestinal, neurological, and developmental damage. According to epidemiological studies, As contributes to male infertility, sexual dysfunction, poor sperm quality, and developmental consequences such as low birth weight, spontaneous abortion, and small for gestational age (SGA). Arsenic exposure negatively affected male reproductive systems by lowering testicular and accessory organ weights, and sperm counts, increasing sperm abnormalities and causing apoptotic cell death in Leydig and Sertoli cells, which resulted in decreased testosterone synthesis. Furthermore, during male reproductive toxicity, several molecular signalling pathways, such as apoptosis, inflammation, and autophagy are involved. Phytonutrient intervention in arsenic-induced male reproductive toxicity in various species has received a lot of attention over the years. The current review provides an in-depth summary of the available literature on arsenic-induced male toxicity, as well as therapeutic approaches and future directions.

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Section: Effects Of Arsenic On the Male Reproductive System In Animalsmentioning

confidence: 99%

Contemporary Comprehensive Review on Arsenic-Induced Male Reproductive Toxicity and Mechanisms of Phytonutrient Intervention

Rachamalla

Chinthada

Kushwaha

et al. 2022

Toxics

View full text Add to dashboard Cite

show abstract

“…Arsenic (As), which is found in nature in trivalent arsenite (As 3+ ) and pentavalent (As 5+ ) arsenate forms, is a highly toxic metalloid formed by volcanic activities and the dissolution of minerals in groundwater 1,2 . It has been reported that the trivalent form of As is more toxic than the pentavalent form 3 . Contact of living things with arsenic occurs orally or inhaled 2 .…”

Section: Introductionmentioning

confidence: 99%

“…As contamination occurs as a result of the production of agricultural pesticides, the production of wood preservatives, glass production, and other mining industry applications, as well as medical applications such as the treatment of leukemia 4,5 . In addition, the most important source of As exposure is the use of drinking water containing inorganic As, 2 and it has been reported that approximately 150 million people worldwide are affected by As‐contaminated water 3 . Acute and chronic exposure to As which has various symptoms such as nausea, vomiting, abdominal pain, encephalopathy, and neuropathy causes toxicity in many tissues due to its water solubility and reaction with endogenous thiol groups and impairs the functions of these tissues 4,6 .…”

Section: Introductionmentioning

confidence: 99%

Carvacrol reduces abnormal and dead sperm counts by attenuating sodium arsenite‐induced oxidative stress, inflammation, apoptosis, and autophagy in the testicular tissues of rats

Gür

Akarsu

Akaras

et al. 2023

Environmental Toxicology

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Background: The present study investigated the protective effects of carvacrol (CAR) against testicular toxicity induced by sodium arsenite (SA).Methods and Results: Rats were given SA and/or CAR for 14 days. Semen analyzes showed that CAR increased sperm motility and decreased the percentage of anarmol and dead sperm. It was determined that the oxidative stress induced by SA decreased with the increase of Nrf-2 and HO-1 expressions, SOD, CAT, GPx and GSH levels, and MDA levels decreased after CAR treatment. It was observed that autophagy and in ammation triggered by SA in testicular tissue were alleviated by suppressing the expressions of LC3A, LC3B, MAPK-14, NF-kB, TNF-a, IL-1b, iNOS and COX-2 biomarkers in rats given CAR. CAR treatment protected against apoptosis by suppressing Bax and Caspase-3 expressions and triggering Bcl-2 expression in testicles.Conclusions: As a result, it was seen that CAR could provide protection against SA-induced damage in testicular tissues of rats.

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“…Previously, we successfully conducted toxicity studies on the reproductive system using the neonatal testis organ culture method [ 24 , 25 ]. Moreover, other studies have also evaluated the toxicity of endocrine disruptor chemicals or toxins on the testes in an in vitro organ culture [ 26 , 27 ]. The organ culture method can reduce the number of test animals and effectively test chemicals and pharmaceuticals on reproductive organ development.…”

Section: Introductionmentioning

confidence: 99%

Cisplatin Induces Apoptosis in Mouse Neonatal Testes Organ Culture

Park

Kim

Lee

et al. 2022

IJMS

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Chemotherapy is used for childhood cancer but may lead to infertility in patients. Spermatogonia stem cells are present in the testes of prepubertal boys, although they do not produce sperm at this age. Herein, we evaluated the toxicity of cisplatin, a known medicine for cancer treatment, in neonatal mouse testes using in vitro organ culture. Mouse testicular fragments (MTFs) derived from 5.5-d postpartum mouse testes were exposed to 1–10 μg/mL cisplatin. The results showed that cisplatin significantly downregulated the expression of germ cell marker genes, including differentiated and undifferentiated, in a dose-dependent manner. In particular, a high dose of cisplatin (10 μg/mL) led to germ cell depletion. In addition, the expression levels of the Sertoli cell marker gene, the number of SOX9+ Sertoli cells, and the levels of SOX9 protein were markedly decreased in cisplatin-treated MTFs compared to controls. The mRNA expression of steroidogenic enzyme-related genes significantly increased in cisplatin-treated MTFs, except for estrogen receptor 1 (Esr1). Consistently, 3β-hydroxysteroid dehydrogenase protein was also observed in the interstitial regions of cisplatin-treated MTFs. Altogether, our findings showed a significant impairment in germ cell development, Sertoli cell survival, and steroidogenesis in the MTFs of cisplatin-treated mice.

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In vitro administration of sodium arsenite in mouse prepubertal testis induces germ cell loss and apoptosis

Abstract: In vitro administration of sodium arsenite in mouse prepubertal testis induces germ cell loss and apoptosis

Cited by 5 publications

References 53 publications

Contemporary Comprehensive Review on Arsenic-Induced Male Reproductive Toxicity and Mechanisms of Phytonutrient Intervention

Contemporary Comprehensive Review on Arsenic-Induced Male Reproductive Toxicity and Mechanisms of Phytonutrient Intervention

Carvacrol reduces abnormal and dead sperm counts by attenuating sodium arsenite‐induced oxidative stress, inflammation, apoptosis, and autophagy in the testicular tissues of rats

Cisplatin Induces Apoptosis in Mouse Neonatal Testes Organ Culture

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