In vitro adventitious organogenesis in Ajania pacifica (Nakai) Bremer et Humphries

Abstract

This study tested the effects of explant type, medium composition, and cultivar on in vitro regeneration of callus, shoots, and roots in Ajania pacifica (Nakai) Bremer et Humphries. In Experiment 1, "Bea", "Bess" and "Bengo" ex vitro internodes and ex vitro leaf explants were inoculated on a modified MS (Murashige and Skoog, 1962) medium with 3 mg @ dm !3 benzylaminopurine (BAP) and 0.5 mg @ dm !3 indole-3-acetic acid (IAA). The share of internodes and leaf explants that proliferated callus were 92-96% and 56-100%, respectively. Adventitious shoot regeneration was observed on "Bengo" and "Bea" internodes (1.12 and 0.32 shoots, respectively, per explant inoculated) and on "Bea" leaf explants (0.2 shoots per explant inoculated). In Experiment 2, "Silver and Gold" ex vitro leaf explants were placed on a modified MS medium with the addition of auxin and cytokinin: 0, 0.5, and 1 mg @ dm !3 1-naphthaleneacetic acid (NAA) or thidiazuron (TDZ) or BAP. Callogenesis was not reported only in the medium without plant growth regulators. Adventitious roots were seen to regenerate on media without plant growth regulators and with 0.5 or 1 mg @ dm !3 NAA (0.47, 1.2, and 2.93 roots per explant inoculated, respectively). In Experiment 3, "Bea", "Bess" and "Bengo" ex vivo internodes were inoculated on a modified MS medium with 0.6 mg @ dm !3 BAP and 2 mg @ dm !3 IAA. All explants produced calli. Shoots regenerated only on "Bengo" internodes (1.3 shoots per explant inoculated). These results confirmed the possibility of in vitro callogenesis and adventitious organogenesis in Ajania pacifica.

Influence of growth regulators on the development, quality, and physiological state of in vitro-propagated Lamprocapnos spectabilis (L.) Fukuhara

“…Their effect depends on the biological material, their concentration, conjugation with other compounds, and the interaction with endogenous and artificial For example, in the study by Pahnekolayi et al (2015), the cytokinin BA alone was the most effective for stimulating shoot proliferation in Rosa canina L. nodal segments, compared with several other combinations of BA, gibberellic acid (GA 3 ), and NAA. NAA auxin (1.0 mg L −1 ) was preferable for stimulation of adventitious shoot regeneration from leaf explants of Ajania pacifica "Silver and Gold," compared with BA or TDZ (Tymoszuk and Antkowiak 2018). For the encapsulated nodal segments of chrysanthemum, the effect of various cytokinins (CKs) was cultivar-specific (Kulus and Zalewska 2014).…”

Induction of Callogenesis, Organogenesis, and Embryogenesis in Non-Meristematic Explants of Bleeding Heart and Evaluation of Chemical Diversity of Key Metabolites from Callus

“…Similarly, cells and tissues of other systematically related species representing Papaveraceae family, e.g., Chelidonium majus L., Eschscholtzia californica Cham., and Papaver somniferum L., have proven to be difficult to culture [25]. Problems with establishing efficient culture systems were reported with several woody species [26], but they are not so often reported with herbaceous plants [27]. The scarce number of adventitious shoots produced in a gross number of PGR combinations tested (including the separate application of BA, KIN, IAA, NAA, and PIC, unpublished data) could result from the small size of bleeding heart explants and indicate the need for evaluating other explant types, e.g., flower petals, roots, or a thin cell layer culture, which worked well with other ornamentals, such as chrysanthemum [28].…”