2011
DOI: 10.1016/j.jmb.2011.10.008
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In Vitro Affinity Maturation of an Anti-PSA Antibody for Prostate Cancer Diagnostic Assay

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Cited by 13 publications
(13 citation statements)
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“…The nonadditive effect of the structure-guided mutations and those achieved by the hot-spot mutagenesis strategy suggests that mAb 5D3D11 may have been optimized in two cases against slightly different PSA subspecies. 10 …”
Section: Discussionmentioning
confidence: 99%
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“…The nonadditive effect of the structure-guided mutations and those achieved by the hot-spot mutagenesis strategy suggests that mAb 5D3D11 may have been optimized in two cases against slightly different PSA subspecies. 10 …”
Section: Discussionmentioning
confidence: 99%
“…Since this complementarity-determining region (CDR) makes no interaction with the antigen, it has been targeted in engineering efforts to improve the antibody's affinity. 10 Another PSA epitopic stretch is 153-159, which runs perpendicular to the 16-26 stretch to which it is linked by a disulfide bond between Cys22 and Cys157. The carbonyl of the cysteine hydrogen bonds to Arg H98 from CDR-H3, which, together with Tyr H97 , interacts with the side chain of Glu159.…”
Section: Klk3/psa-mab 5d3d11: Epitope and Paratopementioning
confidence: 99%
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“…In vitro display and screening over natural or, more often, artificial antibody libraries with phages or other systems have led to the discovery of many valuable antibodies (Bradbury et al, 2011). For antibody affinity maturation, one popular strategy used to generate a synthetic library is targeted mutagenesis of the complementarity determining regions (CDRs) at random or hotspot positions (Barderas et al, 2008;Chowdhury and Pastan, 1999;Laffly et al, 2008;Muller et al, 2011;Rajpal et al, 2005;Wark and Hudson, 2006). In many cases, libraries have been constructed by incorporating degenerate nucleotides at selected positions through solid-phase chemical synthesis of DNA oligonucleotides.…”
Section: Introductionmentioning
confidence: 99%